High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe
Endotoxin (lipopolysaccharide: LPS), a bacterial toxin, is currently quantified by batch analysis using a reagent made from Limulus amebocyte lysate (LAL). Although the LAL method is sensitive, a long measurement time and gelation have hindered its application to online water quality monitoring. In...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Talanta Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831923000255 |
| _version_ | 1797811037633773568 |
|---|---|
| author | Hiroshi Kimoto Yuya Hirahara Kai Sato Masamitsu Iiyama Takeshi Hashimoto Takashi Hayashita |
| author_facet | Hiroshi Kimoto Yuya Hirahara Kai Sato Masamitsu Iiyama Takeshi Hashimoto Takashi Hayashita |
| author_sort | Hiroshi Kimoto |
| collection | DOAJ |
| description | Endotoxin (lipopolysaccharide: LPS), a bacterial toxin, is currently quantified by batch analysis using a reagent made from Limulus amebocyte lysate (LAL). Although the LAL method is sensitive, a long measurement time and gelation have hindered its application to online water quality monitoring. In this paper, we report a novel high-throughput analytical technique that combines flow injection analysis (FIA) and a dipicolylamine-type fluorescent probe, which was previously reported to exhibit strong fluorescence emission in response to LPS. A comparison of normal and reverse FIA systems revealed that the normal FIA offers more rapid detection with throughput of 30 samples per hour, which is the highest among known LAL methods and alternative LPS detection methods that have been recently reported. On the other hand, the reverse FIA requires much less reagent (480 μL per sample). Continuous online monitoring of water quality and analysis of real samples spiked with LPS were realized by this technique. |
| first_indexed | 2024-03-13T07:16:41Z |
| format | Article |
| id | doaj.art-0e96badef03b4a9791ee00371f2c0980 |
| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-03-13T07:16:41Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj.art-0e96badef03b4a9791ee00371f2c09802023-06-05T04:13:17ZengElsevierTalanta Open2666-83192023-08-017100204High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probeHiroshi Kimoto0Yuya Hirahara1Kai Sato2Masamitsu Iiyama3Takeshi Hashimoto4Takashi Hayashita5Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan; Technical Development Division, Nomura Micro Science Co., Ltd., Atsugi, Kanagawa 243-0021, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan; Technical Development Division, Nomura Micro Science Co., Ltd., Atsugi, Kanagawa 243-0021, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, JapanTechnical Development Division, Nomura Micro Science Co., Ltd., Atsugi, Kanagawa 243-0021, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan; Corresponding author.Endotoxin (lipopolysaccharide: LPS), a bacterial toxin, is currently quantified by batch analysis using a reagent made from Limulus amebocyte lysate (LAL). Although the LAL method is sensitive, a long measurement time and gelation have hindered its application to online water quality monitoring. In this paper, we report a novel high-throughput analytical technique that combines flow injection analysis (FIA) and a dipicolylamine-type fluorescent probe, which was previously reported to exhibit strong fluorescence emission in response to LPS. A comparison of normal and reverse FIA systems revealed that the normal FIA offers more rapid detection with throughput of 30 samples per hour, which is the highest among known LAL methods and alternative LPS detection methods that have been recently reported. On the other hand, the reverse FIA requires much less reagent (480 μL per sample). Continuous online monitoring of water quality and analysis of real samples spiked with LPS were realized by this technique.http://www.sciencedirect.com/science/article/pii/S2666831923000255LipopolysaccharideFlow injection analysisDipicolylaminePyreneOnline monitoring |
| spellingShingle | Hiroshi Kimoto Yuya Hirahara Kai Sato Masamitsu Iiyama Takeshi Hashimoto Takashi Hayashita High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe Talanta Open Lipopolysaccharide Flow injection analysis Dipicolylamine Pyrene Online monitoring |
| title | High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe |
| title_full | High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe |
| title_fullStr | High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe |
| title_full_unstemmed | High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe |
| title_short | High-throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine-type fluorescent probe |
| title_sort | high throughput determination of lipopolysaccharide by flow injection analysis using dipicolylamine type fluorescent probe |
| topic | Lipopolysaccharide Flow injection analysis Dipicolylamine Pyrene Online monitoring |
| url | http://www.sciencedirect.com/science/article/pii/S2666831923000255 |
| work_keys_str_mv | AT hiroshikimoto highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe AT yuyahirahara highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe AT kaisato highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe AT masamitsuiiyama highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe AT takeshihashimoto highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe AT takashihayashita highthroughputdeterminationoflipopolysaccharidebyflowinjectionanalysisusingdipicolylaminetypefluorescentprobe |