Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings
The anti-fungal, steroidal, glycoalkaloid saponin, α-tomatine, is present in uninfected tomato plants in substantial concentrations, and may contribute to the protection of tomato plants against attack by phytopathogenic fungi. In general, successful fungal pathogens of tomato are more resistant to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.228 |
| _version_ | 1818915105815920640 |
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| author | Rachel E. Melton Lynda M. Flegg James K. M. Brown Richard P. Oliver Michael J. Daniels Anne E. Osbourn |
| author_facet | Rachel E. Melton Lynda M. Flegg James K. M. Brown Richard P. Oliver Michael J. Daniels Anne E. Osbourn |
| author_sort | Rachel E. Melton |
| collection | DOAJ |
| description | The anti-fungal, steroidal, glycoalkaloid saponin, α-tomatine, is present in uninfected tomato plants in substantial concentrations, and may contribute to the protection of tomato plants against attack by phytopathogenic fungi. In general, successful fungal pathogens of tomato are more resistant to α-tomatine in vitro than fungi that do not infect this plant. For a number of tomato pathogens, this resistance has been associated with the ability to detoxify α-tomatine through the action of enzymes known as tomatinases. In contrast, the biotrophic tomato pathogen Cladosporium fulvum is sensitive to α-tomatine and is unable to detoxify this saponin. This paper describes the effects of heterologous expression of the cDNA encoding tomatinase from the necrotroph Septoria lycopersici in two different physiological races of C. fulvum. Tomatinase-producing C. fulvum transformants showed increased sporulation on cotyledons of susceptible tomato lines. They also caused more extensive infection of seedlings of resistant tomato lines. Thus, α-tomatine may contribute to the ability of tomato to restrict the growth of C. fulvum in both compatible and incompatible interactions. |
| first_indexed | 2024-12-19T23:57:00Z |
| format | Article |
| id | doaj.art-0e974e4c63c24292b5c6071404dfcaec |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:57:00Z |
| publishDate | 1998-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-0e974e4c63c24292b5c6071404dfcaec2022-12-21T20:00:59ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-03-0111322823610.1094/MPMI.1998.11.3.228Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato SeedlingsRachel E. MeltonLynda M. FleggJames K. M. BrownRichard P. OliverMichael J. DanielsAnne E. OsbournThe anti-fungal, steroidal, glycoalkaloid saponin, α-tomatine, is present in uninfected tomato plants in substantial concentrations, and may contribute to the protection of tomato plants against attack by phytopathogenic fungi. In general, successful fungal pathogens of tomato are more resistant to α-tomatine in vitro than fungi that do not infect this plant. For a number of tomato pathogens, this resistance has been associated with the ability to detoxify α-tomatine through the action of enzymes known as tomatinases. In contrast, the biotrophic tomato pathogen Cladosporium fulvum is sensitive to α-tomatine and is unable to detoxify this saponin. This paper describes the effects of heterologous expression of the cDNA encoding tomatinase from the necrotroph Septoria lycopersici in two different physiological races of C. fulvum. Tomatinase-producing C. fulvum transformants showed increased sporulation on cotyledons of susceptible tomato lines. They also caused more extensive infection of seedlings of resistant tomato lines. Thus, α-tomatine may contribute to the ability of tomato to restrict the growth of C. fulvum in both compatible and incompatible interactions.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.228 |
| spellingShingle | Rachel E. Melton Lynda M. Flegg James K. M. Brown Richard P. Oliver Michael J. Daniels Anne E. Osbourn Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings Molecular Plant-Microbe Interactions |
| title | Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings |
| title_full | Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings |
| title_fullStr | Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings |
| title_full_unstemmed | Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings |
| title_short | Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatible and Incompatible Interactions with Tomato Seedlings |
| title_sort | heterologous expression of septoria lycopersici tomatinase in cladosporium fulvum effects on compatible and incompatible interactions with tomato seedlings |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.3.228 |
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