Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature

Bambara groundnut and lima bean are lesser-known legumes with high protein content. These legumes were incorporated into tempeh; a soybean-based snack to advance their uses as food. This research studied effects of the legumes on microbiological, physicochemical and sensory properties of tempeh duri...

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Main Authors: Adanlawo Oladipo Olawale, Malomo Adekunbi Adetola, Adeniran Hezekiah Adekanmi
Format: Article
Language:English
Published: Sciendo 2023-06-01
Series:Acta Universitatis Cibiniensis. Series E: Food Technology
Subjects:
Online Access:https://doi.org/10.2478/aucft-2023-0003
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author Adanlawo Oladipo Olawale
Malomo Adekunbi Adetola
Adeniran Hezekiah Adekanmi
author_facet Adanlawo Oladipo Olawale
Malomo Adekunbi Adetola
Adeniran Hezekiah Adekanmi
author_sort Adanlawo Oladipo Olawale
collection DOAJ
description Bambara groundnut and lima bean are lesser-known legumes with high protein content. These legumes were incorporated into tempeh; a soybean-based snack to advance their uses as food. This research studied effects of the legumes on microbiological, physicochemical and sensory properties of tempeh during storage at ambient and refrigeration temperatures. The results showed that the total viable count of bacteria and fungi count of tempeh mash ranged between 6.173 - 7.903 logcfu/g and 6.602-6.799 log cfu/g respectively. The microbial load of the refrigerated fried tempeh snacks was lower than samples at room temperature. Blends of the three legumes (4.079 – 7.297 logcfu/g) had the highest total viable count while lima bean tempeh (3.903-5.602 logcfu/g) had the lowest. Bacillus cereus, Staphylococcus epidermidis, Bacillus coagulan, Bacillus subtilis, Bacillus alvei, Bacillus Licheniformis, Micrococcus cryophilus, Propionibacterium species, Rhizopus oryzae, Rhizopus stolonifer and Geotricum sp were isolated from tempeh snacks during storage. The results of the sensory assessment showed that lima bean tempeh snack was the most preferred. This research therefore suggests the use of lima bean and blends of lima bean, Bambara groundnut and soybean in production of tempeh to advance the use of these underutilized legumes and increase food security.
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spelling doaj.art-0e9974754ea64c32a0bbdb069fb878682023-09-11T07:01:12ZengSciendoActa Universitatis Cibiniensis. Series E: Food Technology2344-150X2023-06-01271233410.2478/aucft-2023-0003Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage TemperatureAdanlawo Oladipo Olawale0Malomo Adekunbi Adetola1Adeniran Hezekiah Adekanmi2Department of Food Science and Technology, Faculty of Technology, Obafemi Awolowo University, Ile – Ife, NigeriaDepartment of Food Science and Technology, Faculty of Technology, Obafemi Awolowo University, Ile – Ife, NigeriaDepartment of Food Science and Technology, Faculty of Technology, Obafemi Awolowo University, Ile – Ife, NigeriaBambara groundnut and lima bean are lesser-known legumes with high protein content. These legumes were incorporated into tempeh; a soybean-based snack to advance their uses as food. This research studied effects of the legumes on microbiological, physicochemical and sensory properties of tempeh during storage at ambient and refrigeration temperatures. The results showed that the total viable count of bacteria and fungi count of tempeh mash ranged between 6.173 - 7.903 logcfu/g and 6.602-6.799 log cfu/g respectively. The microbial load of the refrigerated fried tempeh snacks was lower than samples at room temperature. Blends of the three legumes (4.079 – 7.297 logcfu/g) had the highest total viable count while lima bean tempeh (3.903-5.602 logcfu/g) had the lowest. Bacillus cereus, Staphylococcus epidermidis, Bacillus coagulan, Bacillus subtilis, Bacillus alvei, Bacillus Licheniformis, Micrococcus cryophilus, Propionibacterium species, Rhizopus oryzae, Rhizopus stolonifer and Geotricum sp were isolated from tempeh snacks during storage. The results of the sensory assessment showed that lima bean tempeh snack was the most preferred. This research therefore suggests the use of lima bean and blends of lima bean, Bambara groundnut and soybean in production of tempeh to advance the use of these underutilized legumes and increase food security.https://doi.org/10.2478/aucft-2023-0003tempehlima beansbambara groundnutrhizopus oryzaelima bean
spellingShingle Adanlawo Oladipo Olawale
Malomo Adekunbi Adetola
Adeniran Hezekiah Adekanmi
Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
Acta Universitatis Cibiniensis. Series E: Food Technology
tempeh
lima beans
bambara groundnut
rhizopus oryzae
lima bean
title Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
title_full Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
title_fullStr Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
title_full_unstemmed Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
title_short Microbiological and Sensory Properties of Tempeh as Influenced by Type of Legumes and Storage Temperature
title_sort microbiological and sensory properties of tempeh as influenced by type of legumes and storage temperature
topic tempeh
lima beans
bambara groundnut
rhizopus oryzae
lima bean
url https://doi.org/10.2478/aucft-2023-0003
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AT adeniranhezekiahadekanmi microbiologicalandsensorypropertiesoftempehasinfluencedbytypeoflegumesandstoragetemperature