Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries
Aim of the study. An actual problem of the modern approach to the management of patients in the post-traumatic period with the aim of predicting, preventing and treating complications of post-traumatic pneumonia is the assessment of the dynamics of the cytokine profile in this period.Material and me...
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| Format: | Article |
| Language: | Russian |
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Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department
2021-08-01
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| Series: | Неотложная медицинская помощь |
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| Online Access: | https://www.jnmp.ru/jour/article/view/1161 |
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| author | A. B. Khalyapina M. V. Parshikov D. D. Bolotov G. M. Zhuravlyova N. V. Yarygin |
| author_facet | A. B. Khalyapina M. V. Parshikov D. D. Bolotov G. M. Zhuravlyova N. V. Yarygin |
| author_sort | A. B. Khalyapina |
| collection | DOAJ |
| description | Aim of the study. An actual problem of the modern approach to the management of patients in the post-traumatic period with the aim of predicting, preventing and treating complications of post-traumatic pneumonia is the assessment of the dynamics of the cytokine profile in this period.Material and methods. Investigations were carried out at the time of hospitalization prior medical interventions. During the research the following methods were used: clinical, physical, instrumental, follow-up, radiation (MRI, CT, X-Ray), endoscopic, laboratory. Blood was taken from the peripheral vein on the first, third, fifth, seventh and ninth day of hospitalization to determine the interlikin profile. The definition of Proinflammatory cytokines: IL-1, IL-6 and IL-10 in the blood serum performed by ELISA on the immunofermental analyzer Abbott AXSYM using standard kits ProCon (“Protein contour”, St. Petersburg, Russia). Data were processed statistically using Student’s t-test.Results. Analysis of the results of determination of IL-1 in the serum of patients DK showed that in all stages of the disease, in addition to IV, the contents of this cytokine exceeded the control values in varying degrees of severity. While the levels of IL-1 in the serum of patients with the 1ststage of the disease was 4.0 times; stage 2–3.4 times; the third stage is 1.5 times higher than normal. And only in patients with very severe stage of pneumonia contents IL-1 did not differ from the norm. A similar picture was observed when determining the serum content of patients with a very severe stage of pneumonia, and only in contrast to the level of IL-1 in patients with a very severe stage of pneumonia, the IL-6 content remained significantly higher than normal. So, the content of IL-10 in patients with a very severe stage of pneumonia of stage I, II, III and IV of the disease averaged 330.7±24.5, respectively; 210.5±17.3; 123.4±15.3 and 98.5±12.7 pg/ml (in all cases p<0.05). CONCLUSION The results obtained in a comparative study of contents of proinflammatory cytokines (IL-1, IL-6 and TNF-a) in the blood serum showed that the determination of the spectrum of cytokines in peripheral blood, in our opinion, is the most perspective and can be a key marker for early detection of inflammation. Key words: post-traumatic pneumonii, interlikin profile, peripheral blood serum>˂0.05).Conclusion. The results obtained in a comparative study of contents of proinflammatory cytokines (IL-1, IL-6 and TNF-a) in the blood serum showed that the determination of the spectrum of cytokines in peripheral blood, in our opinion, is the most perspective and can be a key marker for early detection of inflammation. |
| first_indexed | 2024-03-12T05:34:06Z |
| format | Article |
| id | doaj.art-0e9a1877cc3740488c35714d1f9d1258 |
| institution | Directory Open Access Journal |
| issn | 2223-9022 2541-8017 |
| language | Russian |
| last_indexed | 2024-03-12T05:34:06Z |
| publishDate | 2021-08-01 |
| publisher | Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department |
| record_format | Article |
| series | Неотложная медицинская помощь |
| spelling | doaj.art-0e9a1877cc3740488c35714d1f9d12582023-09-03T06:32:51ZrusSklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare DepartmentНеотложная медицинская помощь2223-90222541-80172021-08-0110231832710.23934/2223-9022-2021-10-2-318-327686Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest InjuriesA. B. Khalyapina0M. V. Parshikov1D. D. Bolotov2G. M. Zhuravlyova3N. V. Yarygin4A.I. Evdokimov Moscow State University of Medicine and DentistryA.I. Evdokimov Moscow State University of Medicine and DentistryA.I. Evdokimov Moscow State University of Medicine and DentistryA.I. Evdokimov Moscow State University of Medicine and DentistryA.I. Evdokimov Moscow State University of Medicine and DentistryAim of the study. An actual problem of the modern approach to the management of patients in the post-traumatic period with the aim of predicting, preventing and treating complications of post-traumatic pneumonia is the assessment of the dynamics of the cytokine profile in this period.Material and methods. Investigations were carried out at the time of hospitalization prior medical interventions. During the research the following methods were used: clinical, physical, instrumental, follow-up, radiation (MRI, CT, X-Ray), endoscopic, laboratory. Blood was taken from the peripheral vein on the first, third, fifth, seventh and ninth day of hospitalization to determine the interlikin profile. The definition of Proinflammatory cytokines: IL-1, IL-6 and IL-10 in the blood serum performed by ELISA on the immunofermental analyzer Abbott AXSYM using standard kits ProCon (“Protein contour”, St. Petersburg, Russia). Data were processed statistically using Student’s t-test.Results. Analysis of the results of determination of IL-1 in the serum of patients DK showed that in all stages of the disease, in addition to IV, the contents of this cytokine exceeded the control values in varying degrees of severity. While the levels of IL-1 in the serum of patients with the 1ststage of the disease was 4.0 times; stage 2–3.4 times; the third stage is 1.5 times higher than normal. And only in patients with very severe stage of pneumonia contents IL-1 did not differ from the norm. A similar picture was observed when determining the serum content of patients with a very severe stage of pneumonia, and only in contrast to the level of IL-1 in patients with a very severe stage of pneumonia, the IL-6 content remained significantly higher than normal. So, the content of IL-10 in patients with a very severe stage of pneumonia of stage I, II, III and IV of the disease averaged 330.7±24.5, respectively; 210.5±17.3; 123.4±15.3 and 98.5±12.7 pg/ml (in all cases p<0.05). CONCLUSION The results obtained in a comparative study of contents of proinflammatory cytokines (IL-1, IL-6 and TNF-a) in the blood serum showed that the determination of the spectrum of cytokines in peripheral blood, in our opinion, is the most perspective and can be a key marker for early detection of inflammation. Key words: post-traumatic pneumonii, interlikin profile, peripheral blood serum>˂0.05).Conclusion. The results obtained in a comparative study of contents of proinflammatory cytokines (IL-1, IL-6 and TNF-a) in the blood serum showed that the determination of the spectrum of cytokines in peripheral blood, in our opinion, is the most perspective and can be a key marker for early detection of inflammation.https://www.jnmp.ru/jour/article/view/1161post-traumatic pneumoniiinterlikin profileperipheral blood serum |
| spellingShingle | A. B. Khalyapina M. V. Parshikov D. D. Bolotov G. M. Zhuravlyova N. V. Yarygin Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries Неотложная медицинская помощь post-traumatic pneumonii interlikin profile peripheral blood serum |
| title | Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries |
| title_full | Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries |
| title_fullStr | Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries |
| title_full_unstemmed | Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries |
| title_short | Evaluation of Cytokine Dynamics in Patients With Multiple and Concomitant Closed Chest Injuries |
| title_sort | evaluation of cytokine dynamics in patients with multiple and concomitant closed chest injuries |
| topic | post-traumatic pneumonii interlikin profile peripheral blood serum |
| url | https://www.jnmp.ru/jour/article/view/1161 |
| work_keys_str_mv | AT abkhalyapina evaluationofcytokinedynamicsinpatientswithmultipleandconcomitantclosedchestinjuries AT mvparshikov evaluationofcytokinedynamicsinpatientswithmultipleandconcomitantclosedchestinjuries AT ddbolotov evaluationofcytokinedynamicsinpatientswithmultipleandconcomitantclosedchestinjuries AT gmzhuravlyova evaluationofcytokinedynamicsinpatientswithmultipleandconcomitantclosedchestinjuries AT nvyarygin evaluationofcytokinedynamicsinpatientswithmultipleandconcomitantclosedchestinjuries |