Effect of pesticides on erythrocytes of indigenous fish Labeo rohita
Single cell gel electrophoresis was used to observe the effect of bifenthrin and chlorpyrifos on Labeo rohita. The present work was planned to study the genotoxic effect of pesticide mixture on the major indian carp, L. rohita at sub-lethal concentration 33% LC50. First of all, 96-hr LC50 was determ...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721002482 |
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| author | Naheed Bano Amir Nadeem Sadia Maalik Sajida Mushtaq Naeem Iqbal Ahmad Kamran Khan Asghar Shabbir Muhammad Imran Safia Niaz Muhammad Yasin Mirza Abdul Qayyum Bashir Ahmad Milan Skalicky Sulaiman Ali Alharbi Saleh Alfarraj |
| author_facet | Naheed Bano Amir Nadeem Sadia Maalik Sajida Mushtaq Naeem Iqbal Ahmad Kamran Khan Asghar Shabbir Muhammad Imran Safia Niaz Muhammad Yasin Mirza Abdul Qayyum Bashir Ahmad Milan Skalicky Sulaiman Ali Alharbi Saleh Alfarraj |
| author_sort | Naheed Bano |
| collection | DOAJ |
| description | Single cell gel electrophoresis was used to observe the effect of bifenthrin and chlorpyrifos on Labeo rohita. The present work was planned to study the genotoxic effect of pesticide mixture on the major indian carp, L. rohita at sub-lethal concentration 33% LC50. First of all, 96-hr LC50 was determined for L. rohita, then fish was given sub-lethal environment for 70 days. Erythrocytes from blood were taken after each fortnight for the assessment of time dependent DNA damage (% damage), aggregate tail length of comets and genomic destruction. There was increase in damage up to 56 days and after that a slight decrease was observed in next fourteen days. After statistical analysis, it was concluded that the Single Cell Gel Electrophoresis may be implemented in ecofriendly observer courses in evaluation of toxicity. |
| first_indexed | 2024-12-17T23:57:16Z |
| format | Article |
| id | doaj.art-0e9b29426b6b409a824be1174034aa31 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-17T23:57:16Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-0e9b29426b6b409a824be1174034aa312022-12-21T21:28:02ZengElsevierJournal of King Saud University: Science1018-36472021-10-01337101586Effect of pesticides on erythrocytes of indigenous fish Labeo rohitaNaheed Bano0Amir Nadeem1Sadia Maalik2Sajida Mushtaq3Naeem Iqbal4Ahmad Kamran Khan5Asghar Shabbir6Muhammad Imran7Safia Niaz8Muhammad Yasin9Mirza Abdul Qayyum10Bashir Ahmad11Milan Skalicky12Sulaiman Ali Alharbi13Saleh Alfarraj14Department of Veterinary and Animal Sciences, MNS University of Agriculture, Multan, Pakistan; Corresponding author at: Department of Veterinary and Animal Sciences, MNS University of Agriculture, Multan, Pakistan.Department of Zoology, Govt. College University, LahoreDepartment of Zoology, G.C Women University, Sialkot, PakistanDepartment of Zoology, G.C Women University, Sialkot, PakistanInstitute of Plant Protection, MNS University of Agriculture, Multan, PakistanDepartment of Plant Protection, Ghazi University, Dera Ghazi Khan, PakistanDepartment of Biosciences, COMSATS University Islamabad, Park Road, Tarlai Kalan 45550, Islamabad, PakistanDepartment of Entomology, Faculty of Agriculture, University of Poonch Rawalakot Azad Jammu and Kashmir, PakistanDepartment of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, PakistanDepartment of Entomology, The Islamia University of Bahawalpur, PakistanInstitute of Plant Protection, MNS University of Agriculture, Multan, PakistanDepartment of Zoology, The University of Haripur, Khyber Pakhtunkhwa, PakistanDepartment of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Prague, CzechiaDept. of Botany & Microbiology, College of Science, King Saud University, P.O Box 2455, Riyadh 1145, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaSingle cell gel electrophoresis was used to observe the effect of bifenthrin and chlorpyrifos on Labeo rohita. The present work was planned to study the genotoxic effect of pesticide mixture on the major indian carp, L. rohita at sub-lethal concentration 33% LC50. First of all, 96-hr LC50 was determined for L. rohita, then fish was given sub-lethal environment for 70 days. Erythrocytes from blood were taken after each fortnight for the assessment of time dependent DNA damage (% damage), aggregate tail length of comets and genomic destruction. There was increase in damage up to 56 days and after that a slight decrease was observed in next fourteen days. After statistical analysis, it was concluded that the Single Cell Gel Electrophoresis may be implemented in ecofriendly observer courses in evaluation of toxicity.http://www.sciencedirect.com/science/article/pii/S1018364721002482L. rohitaDNA destructionPesticidal mixtureSingle cell gel electrophoresis |
| spellingShingle | Naheed Bano Amir Nadeem Sadia Maalik Sajida Mushtaq Naeem Iqbal Ahmad Kamran Khan Asghar Shabbir Muhammad Imran Safia Niaz Muhammad Yasin Mirza Abdul Qayyum Bashir Ahmad Milan Skalicky Sulaiman Ali Alharbi Saleh Alfarraj Effect of pesticides on erythrocytes of indigenous fish Labeo rohita Journal of King Saud University: Science L. rohita DNA destruction Pesticidal mixture Single cell gel electrophoresis |
| title | Effect of pesticides on erythrocytes of indigenous fish Labeo rohita |
| title_full | Effect of pesticides on erythrocytes of indigenous fish Labeo rohita |
| title_fullStr | Effect of pesticides on erythrocytes of indigenous fish Labeo rohita |
| title_full_unstemmed | Effect of pesticides on erythrocytes of indigenous fish Labeo rohita |
| title_short | Effect of pesticides on erythrocytes of indigenous fish Labeo rohita |
| title_sort | effect of pesticides on erythrocytes of indigenous fish labeo rohita |
| topic | L. rohita DNA destruction Pesticidal mixture Single cell gel electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721002482 |
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