The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins
As one of the main functions of vascular endothelial cells, Vascular permeability is determined by four tight junction proteins (TJPs): Zonula Occludens-1 (ZO-1), Claudin-5, Occludin and Tricellulin. The barrier function of blood vessels will be reconstructed after they are damaged by endothelial me...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422002083 |
| _version_ | 1811235247766896640 |
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| author | Junyang Huang Shuang Ge Desha Luo Ruolin Du Yang Wang Wanling Liu Guixue Wang Tieying Yin |
| author_facet | Junyang Huang Shuang Ge Desha Luo Ruolin Du Yang Wang Wanling Liu Guixue Wang Tieying Yin |
| author_sort | Junyang Huang |
| collection | DOAJ |
| description | As one of the main functions of vascular endothelial cells, Vascular permeability is determined by four tight junction proteins (TJPs): Zonula Occludens-1 (ZO-1), Claudin-5, Occludin and Tricellulin. The barrier function of blood vessels will be reconstructed after they are damaged by endothelial mechanical injuries caused by vascular interventions. In this study, the effects of balloon expansion (transient mechanical injury) on four TJPs and vascular permeability were compared with those of poly-l-lactic acid bioresorbable scaffolds (BRSs) implantation (continuous mechanical stimulation). We found that BRSs do not affect vascular permeability, while the recovery of vascular barrier function was found to be only related to the mechanical injuries and repair of endothelium. Mechanical stimulation affects and accelerates the recovery process of vascular permeability with the heterogeneous expression levels of TJPs induced after BRSs implantation. Different TJPs have different sensitivity to different loyal mechanical stimuli. ZO-1 is more sensitive to shear stress and tension than to static pressure. Occludin is sensitive to static pressure and shear stress. Tricellulin is more sensitive to tension stretching. Compared with the other three TJPs, Claudin-5 can respond to mechanical stimulation, with relatively low sensitivity, though. This difference in sensitivity determines the heterogeneous expression of TJPs. Mechanical stimulation of different kinds and strengths can also cause different cell morphological changes and inflammatory reactions. As an important element affecting endothelial function, the mechanical factors emerging after BRSs implantation are worthy of more attention. |
| first_indexed | 2024-04-12T11:47:56Z |
| format | Article |
| id | doaj.art-0e9f05b519604692ac732eb5754eff72 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-12T11:47:56Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-0e9f05b519604692ac732eb5754eff722022-12-22T03:34:16ZengElsevierMaterials Today Bio2590-00642022-12-0116100410The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteinsJunyang Huang0Shuang Ge1Desha Luo2Ruolin Du3Yang Wang4Wanling Liu5Guixue Wang6Tieying Yin7Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaCorresponding author.; Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaCorresponding author.; Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, ChinaAs one of the main functions of vascular endothelial cells, Vascular permeability is determined by four tight junction proteins (TJPs): Zonula Occludens-1 (ZO-1), Claudin-5, Occludin and Tricellulin. The barrier function of blood vessels will be reconstructed after they are damaged by endothelial mechanical injuries caused by vascular interventions. In this study, the effects of balloon expansion (transient mechanical injury) on four TJPs and vascular permeability were compared with those of poly-l-lactic acid bioresorbable scaffolds (BRSs) implantation (continuous mechanical stimulation). We found that BRSs do not affect vascular permeability, while the recovery of vascular barrier function was found to be only related to the mechanical injuries and repair of endothelium. Mechanical stimulation affects and accelerates the recovery process of vascular permeability with the heterogeneous expression levels of TJPs induced after BRSs implantation. Different TJPs have different sensitivity to different loyal mechanical stimuli. ZO-1 is more sensitive to shear stress and tension than to static pressure. Occludin is sensitive to static pressure and shear stress. Tricellulin is more sensitive to tension stretching. Compared with the other three TJPs, Claudin-5 can respond to mechanical stimulation, with relatively low sensitivity, though. This difference in sensitivity determines the heterogeneous expression of TJPs. Mechanical stimulation of different kinds and strengths can also cause different cell morphological changes and inflammatory reactions. As an important element affecting endothelial function, the mechanical factors emerging after BRSs implantation are worthy of more attention.http://www.sciencedirect.com/science/article/pii/S2590006422002083Bioresorbable scaffolds (BRSs)Poly-l-lactic acid (PLLA)Tight junction proteins (TJPs)Endothelium permeabilityMechanical stimulation |
| spellingShingle | Junyang Huang Shuang Ge Desha Luo Ruolin Du Yang Wang Wanling Liu Guixue Wang Tieying Yin The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins Materials Today Bio Bioresorbable scaffolds (BRSs) Poly-l-lactic acid (PLLA) Tight junction proteins (TJPs) Endothelium permeability Mechanical stimulation |
| title | The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| title_full | The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| title_fullStr | The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| title_full_unstemmed | The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| title_short | The endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| title_sort | endothelium permeability after bioresorbable scaffolds implantation caused by the heterogeneous expression of tight junction proteins |
| topic | Bioresorbable scaffolds (BRSs) Poly-l-lactic acid (PLLA) Tight junction proteins (TJPs) Endothelium permeability Mechanical stimulation |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422002083 |
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