Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa
<p>Abstract</p> <p>Background</p> <p>Malaria parasite population genetic structure varies among areas of differing endemicity, but this has not been systematically studied across <it>Plasmodium falciparum</it> populations in Africa where most infections occu...
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| Format: | Article |
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BMC
2012-07-01
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| Series: | Malaria Journal |
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| Online Access: | http://www.malariajournal.com/content/11/1/223 |
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| author | Mobegi Victor A Loua Kovana M Ahouidi Ambroise D Satoguina Judith Nwakanma Davis C Amambua-Ngwa Alfred Conway David J |
| author_facet | Mobegi Victor A Loua Kovana M Ahouidi Ambroise D Satoguina Judith Nwakanma Davis C Amambua-Ngwa Alfred Conway David J |
| author_sort | Mobegi Victor A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Malaria parasite population genetic structure varies among areas of differing endemicity, but this has not been systematically studied across <it>Plasmodium falciparum</it> populations in Africa where most infections occur.</p> <p>Methods</p> <p>Ten polymorphic <it>P. falciparum</it> microsatellite loci were genotyped in 268 infections from eight locations in four West African countries (Republic of Guinea, Guinea Bissau, The Gambia and Senegal), spanning a highly endemic forested region in the south to a low endemic Sahelian region in the north. Analysis was performed on proportions of mixed genotype infections, genotypic diversity among isolates, multilocus standardized index of association, and inter-population differentiation.</p> <p>Results</p> <p>Each location had similar levels of pairwise genotypic diversity among isolates, although there were many more mixed parasite genotype infections in the south. Apart from a few isolates that were virtually identical, the multilocus index of association was not significant in any population. Genetic differentiation between populations was low (most pairwise <it>F</it><sub>ST</sub> values < 0.03), and an overall test for isolation by distance was not significant.</p> <p>Conclusions</p> <p>Although proportions of mixed genotype infections varied with endemicity as expected, population genetic structure was similar across the diverse sites. Very substantial reduction in transmission would be needed to cause fragmented or epidemic sub-structure in this region.</p> |
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| id | doaj.art-0ea3f7ebc6b749fa835fa27f8bd33464 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-19T02:29:30Z |
| publishDate | 2012-07-01 |
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| series | Malaria Journal |
| spelling | doaj.art-0ea3f7ebc6b749fa835fa27f8bd334642022-12-21T20:39:41ZengBMCMalaria Journal1475-28752012-07-0111122310.1186/1475-2875-11-223Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West AfricaMobegi Victor ALoua Kovana MAhouidi Ambroise DSatoguina JudithNwakanma Davis CAmambua-Ngwa AlfredConway David J<p>Abstract</p> <p>Background</p> <p>Malaria parasite population genetic structure varies among areas of differing endemicity, but this has not been systematically studied across <it>Plasmodium falciparum</it> populations in Africa where most infections occur.</p> <p>Methods</p> <p>Ten polymorphic <it>P. falciparum</it> microsatellite loci were genotyped in 268 infections from eight locations in four West African countries (Republic of Guinea, Guinea Bissau, The Gambia and Senegal), spanning a highly endemic forested region in the south to a low endemic Sahelian region in the north. Analysis was performed on proportions of mixed genotype infections, genotypic diversity among isolates, multilocus standardized index of association, and inter-population differentiation.</p> <p>Results</p> <p>Each location had similar levels of pairwise genotypic diversity among isolates, although there were many more mixed parasite genotype infections in the south. Apart from a few isolates that were virtually identical, the multilocus index of association was not significant in any population. Genetic differentiation between populations was low (most pairwise <it>F</it><sub>ST</sub> values < 0.03), and an overall test for isolation by distance was not significant.</p> <p>Conclusions</p> <p>Although proportions of mixed genotype infections varied with endemicity as expected, population genetic structure was similar across the diverse sites. Very substantial reduction in transmission would be needed to cause fragmented or epidemic sub-structure in this region.</p>http://www.malariajournal.com/content/11/1/223<it>Plasmodium falciparum</it>MicrosatellitePopulation structureTransmission intensity |
| spellingShingle | Mobegi Victor A Loua Kovana M Ahouidi Ambroise D Satoguina Judith Nwakanma Davis C Amambua-Ngwa Alfred Conway David J Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa Malaria Journal <it>Plasmodium falciparum</it> Microsatellite Population structure Transmission intensity |
| title | Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa |
| title_full | Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa |
| title_fullStr | Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa |
| title_full_unstemmed | Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa |
| title_short | Population genetic structure of <it>Plasmodium falciparum</it> across a region of diverse endemicity in West Africa |
| title_sort | population genetic structure of it plasmodium falciparum it across a region of diverse endemicity in west africa |
| topic | <it>Plasmodium falciparum</it> Microsatellite Population structure Transmission intensity |
| url | http://www.malariajournal.com/content/11/1/223 |
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