Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation
The effects of ankaflavin (AK) on adipokines level and insulin sensitivity were investigated. Mice were fed with high-fat diet (HFD) and AK to evaluate hyperglycemia and hyperlipidemia. Additionally, mature differentiated 3T3-L1 adipocytes were treated with AK or monascin (MS) to determine insulin s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464612001417 |
| _version_ | 1818412069511233536 |
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| author | Wei-Hsuan Hsu Te-Han Liao Bao-Hong Lee Ya-Wen Hsu Tzu-Ming Pan |
| author_facet | Wei-Hsuan Hsu Te-Han Liao Bao-Hong Lee Ya-Wen Hsu Tzu-Ming Pan |
| author_sort | Wei-Hsuan Hsu |
| collection | DOAJ |
| description | The effects of ankaflavin (AK) on adipokines level and insulin sensitivity were investigated. Mice were fed with high-fat diet (HFD) and AK to evaluate hyperglycemia and hyperlipidemia. Additionally, mature differentiated 3T3-L1 adipocytes were treated with AK or monascin (MS) to determine insulin signaling. Results showed that AK down-regulated serum and hepatic triacylglyceride (TG) and total cholesterol (TC) levels in mice, ameliorating hyperglycemia and hyperinsulinemia symptoms elicited by the HFD. AK elevated serum adiponectin levels in HFD-induced mice and mature 3T3-L1 adipocytes. The effect of AK on insulin sensitivity and adipokines secretion were abolished by peroxisome proliferator activated receptor-γ PPAR-γ antagonist GW9662. Moreover, we made the novel discovery that AK markedly promoted insulin receptor and Akt phosphorylation, and increased glucose uptake in mature 3T3-L1 adipocytes to a greater extent than did MS. Taken together, AK attenuated insulin resistance in HFD-induced mice and promoted insulin sensitivity in 3T3-L1 adipocytes through PPAR-γ activation. |
| first_indexed | 2024-12-14T10:41:27Z |
| format | Article |
| id | doaj.art-0ea4a91479574fe5a095152ebd705bf4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:41:27Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-0ea4a91479574fe5a095152ebd705bf42022-12-21T23:05:40ZengElsevierJournal of Functional Foods1756-46462013-01-0151124132Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activationWei-Hsuan Hsu0Te-Han Liao1Bao-Hong Lee2Ya-Wen Hsu3Tzu-Ming Pan4Department of Biochemical Science & Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, TaiwanDepartment of Biochemical Science & Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, TaiwanDepartment of Biochemical Science & Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, TaiwanDepartment of Biochemical Science & Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, TaiwanCorresponding author. Tel.: +886 2 33664519x10; fax: +886 2 33663838.; Department of Biochemical Science & Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, TaiwanThe effects of ankaflavin (AK) on adipokines level and insulin sensitivity were investigated. Mice were fed with high-fat diet (HFD) and AK to evaluate hyperglycemia and hyperlipidemia. Additionally, mature differentiated 3T3-L1 adipocytes were treated with AK or monascin (MS) to determine insulin signaling. Results showed that AK down-regulated serum and hepatic triacylglyceride (TG) and total cholesterol (TC) levels in mice, ameliorating hyperglycemia and hyperinsulinemia symptoms elicited by the HFD. AK elevated serum adiponectin levels in HFD-induced mice and mature 3T3-L1 adipocytes. The effect of AK on insulin sensitivity and adipokines secretion were abolished by peroxisome proliferator activated receptor-γ PPAR-γ antagonist GW9662. Moreover, we made the novel discovery that AK markedly promoted insulin receptor and Akt phosphorylation, and increased glucose uptake in mature 3T3-L1 adipocytes to a greater extent than did MS. Taken together, AK attenuated insulin resistance in HFD-induced mice and promoted insulin sensitivity in 3T3-L1 adipocytes through PPAR-γ activation.http://www.sciencedirect.com/science/article/pii/S1756464612001417AnkaflavinPeroxisome proliferator activated receptor-γ (PPAR-γ)High-fat dietGW9662Adipokines |
| spellingShingle | Wei-Hsuan Hsu Te-Han Liao Bao-Hong Lee Ya-Wen Hsu Tzu-Ming Pan Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation Journal of Functional Foods Ankaflavin Peroxisome proliferator activated receptor-γ (PPAR-γ) High-fat diet GW9662 Adipokines |
| title | Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation |
| title_full | Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation |
| title_fullStr | Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation |
| title_full_unstemmed | Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation |
| title_short | Ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high-fat diet via PPAR-γ activation |
| title_sort | ankaflavin regulates adipocyte function and attenuates hyperglycemia caused by high fat diet via ppar γ activation |
| topic | Ankaflavin Peroxisome proliferator activated receptor-γ (PPAR-γ) High-fat diet GW9662 Adipokines |
| url | http://www.sciencedirect.com/science/article/pii/S1756464612001417 |
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