Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases
Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition...
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| Format: | Article |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00295/full |
| _version_ | 1819137246077386752 |
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| author | Yuan Sui Michael Wisniewski Samir Droby Edoardo Piombo Xuehong Wu Junyang Yue |
| author_facet | Yuan Sui Michael Wisniewski Samir Droby Edoardo Piombo Xuehong Wu Junyang Yue |
| author_sort | Yuan Sui |
| collection | DOAJ |
| description | Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and space, induction of pathogenesis-related genes in host tissues, and production of extracellular lytic enzymes, have been demonstrated for the biological control activity exhibited by C. oleophila through which it inhibits post-harvest pathogens. In the present study, the whole genome of C. oleophila I-182 was sequenced using PacBio and Illumina shotgun sequencing technologies, yielding an estimated genome size of 14.73 Mb. The genome size is similar in length to that of the model yeast strain Saccharomyces cerevisiae S288c. Based on the assembled genome, protein-coding sequences were identified and annotated. The predicted genes were further assigned with gene ontology terms and clustered in special functional groups. A comparative analysis of C. oleophila proteome with the proteomes of 11 representative yeasts revealed 2 unique and 124 expanded families of proteins in C. oleophila. Availability of the genome sequence will facilitate a better understanding the properties of biocontrol yeasts at the molecular level. |
| first_indexed | 2024-12-22T10:47:49Z |
| format | Article |
| id | doaj.art-0ea50b4880114ad890f59ff23c6cad62 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T10:47:49Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0ea50b4880114ad890f59ff23c6cad622022-12-21T18:28:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-02-011110.3389/fmicb.2020.00295518395Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest DiseasesYuan Sui0Michael Wisniewski1Samir Droby2Edoardo Piombo3Xuehong Wu4Junyang Yue5Chongqing Key Laboratory of Economic Plant Biotechnology, Collaborative Innovation Center of Special Plant Industry in Chongqing, College of Forestry and Life Science, Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, ChinaU.S. Department of Agriculture-Agricultural Research Service, Kearneysville, WV, United StatesDepartment of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, IsraelDepartment of Agricultural, Forestry and Food Sciences, University of Turin, Turin, ItalyDepartment of Plant Pathology, China Agricultural University, Beijing, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaCandida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and space, induction of pathogenesis-related genes in host tissues, and production of extracellular lytic enzymes, have been demonstrated for the biological control activity exhibited by C. oleophila through which it inhibits post-harvest pathogens. In the present study, the whole genome of C. oleophila I-182 was sequenced using PacBio and Illumina shotgun sequencing technologies, yielding an estimated genome size of 14.73 Mb. The genome size is similar in length to that of the model yeast strain Saccharomyces cerevisiae S288c. Based on the assembled genome, protein-coding sequences were identified and annotated. The predicted genes were further assigned with gene ontology terms and clustered in special functional groups. A comparative analysis of C. oleophila proteome with the proteomes of 11 representative yeasts revealed 2 unique and 124 expanded families of proteins in C. oleophila. Availability of the genome sequence will facilitate a better understanding the properties of biocontrol yeasts at the molecular level.https://www.frontiersin.org/article/10.3389/fmicb.2020.00295/fullbiocontrol agentCandida oleophilagenome assemblygenome annotationpost-harvest disease management |
| spellingShingle | Yuan Sui Michael Wisniewski Samir Droby Edoardo Piombo Xuehong Wu Junyang Yue Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases Frontiers in Microbiology biocontrol agent Candida oleophila genome assembly genome annotation post-harvest disease management |
| title | Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases |
| title_full | Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases |
| title_fullStr | Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases |
| title_full_unstemmed | Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases |
| title_short | Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases |
| title_sort | genome sequence assembly and characterization of the antagonistic yeast candida oleophila used as a biocontrol agent against post harvest diseases |
| topic | biocontrol agent Candida oleophila genome assembly genome annotation post-harvest disease management |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00295/full |
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