CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients
Abstract. Patients with nonalcoholic fatty liver disease (NAFLD) have symptoms of a gut microbiota disorder with abnormal amino acid and glycolipid metabolism. This study was designed to analyze the characteristics of gut microbiota in patients with NAFLD, predict the gut microbiota phenotype, explo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2022-06-01
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| Series: | Medicine |
| Online Access: | http://journals.lww.com/10.1097/MD.0000000000029347 |
| _version_ | 1811236917303312384 |
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| author | Haize Ge, PhD Wei Wei, MD Liang Tang, PhD Yaqiong Tian, BD Yu Zhu, PhD Yan Luo, MD Shuye Liu, BD |
| author_facet | Haize Ge, PhD Wei Wei, MD Liang Tang, PhD Yaqiong Tian, BD Yu Zhu, PhD Yan Luo, MD Shuye Liu, BD |
| author_sort | Haize Ge, PhD |
| collection | DOAJ |
| description | Abstract. Patients with nonalcoholic fatty liver disease (NAFLD) have symptoms of a gut microbiota disorder with abnormal amino acid and glycolipid metabolism. This study was designed to analyze the characteristics of gut microbiota in patients with NAFLD, predict the gut microbiota phenotype, explore its role in the diagnosis of NAFLD, and establish its significance in disease progression.
The characteristics of the gut microbiota in NAFLD patients (n = 28, 45.8 ± 14.2 years, male/female = 18/10) and healthy subjects (n = 20, 49.6 ± 4.8 years, male/female = 14/6) during March–May 2020 were analyzed using 16S rRNA sequencing technology and the phenotypes with large differences were predicted using the Tax4Fun method. The metabolites in the fecal samples of the patients were analyzed using mass spectrometry, and their correlation with different microorganisms was examined. The accuracy of the gut microbiota in diagnosing NAFLD was investigated by receiver operating characteristic curve analysis.
We found that the microbial diversity and Bacteroides/Firmicutes (BF) ratio changed significantly (P < .05) in the feces of NAFLD patients. Phenotypic prediction showed that there were significant differences in the phenotypes of amino acid, glucose, and lipid metabolism of gut microbiota in the NAFLD group (P < .05). receiver operating characteristic curve analysis revealed that combination of Bacteroides and the BF ratio resulted in 88% and 100% sensitivity and specificity, respectively, when used for NAFLD diagnosis. Metabolomics and bioinformatics analysis revealed changes in the metabolism of nicotinate, nicotinamide, and pyrimidine; signaling pathways of calcium and oxytocin; pancreatic secretion with metabolites such as uracil, xanthine, and biliverdin; and enzymes such as xanthine dehydrogenase and xanthine oxidase (P < .05).
Therefore, the phenotypic changes may be a potential marker for NAFLD and we considered that a combined analysis of Bacteroides and BF ratio had good diagnostic accuracy for NAFLD. |
| first_indexed | 2024-04-12T12:16:22Z |
| format | Article |
| id | doaj.art-0ea50f6bf9a5438580bf10b129eb38a4 |
| institution | Directory Open Access Journal |
| issn | 0025-7974 1536-5964 |
| language | English |
| last_indexed | 2024-04-12T12:16:22Z |
| publishDate | 2022-06-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Medicine |
| spelling | doaj.art-0ea50f6bf9a5438580bf10b129eb38a42022-12-22T03:33:24ZengWolters KluwerMedicine0025-79741536-59642022-06-0110125e2934710.1097/MD.0000000000029347202206240-00035CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patientsHaize Ge, PhDWei Wei, MDLiang Tang, PhDYaqiong Tian, BDYu Zhu, PhDYan Luo, MDShuye Liu, BDAbstract. Patients with nonalcoholic fatty liver disease (NAFLD) have symptoms of a gut microbiota disorder with abnormal amino acid and glycolipid metabolism. This study was designed to analyze the characteristics of gut microbiota in patients with NAFLD, predict the gut microbiota phenotype, explore its role in the diagnosis of NAFLD, and establish its significance in disease progression. The characteristics of the gut microbiota in NAFLD patients (n = 28, 45.8 ± 14.2 years, male/female = 18/10) and healthy subjects (n = 20, 49.6 ± 4.8 years, male/female = 14/6) during March–May 2020 were analyzed using 16S rRNA sequencing technology and the phenotypes with large differences were predicted using the Tax4Fun method. The metabolites in the fecal samples of the patients were analyzed using mass spectrometry, and their correlation with different microorganisms was examined. The accuracy of the gut microbiota in diagnosing NAFLD was investigated by receiver operating characteristic curve analysis. We found that the microbial diversity and Bacteroides/Firmicutes (BF) ratio changed significantly (P < .05) in the feces of NAFLD patients. Phenotypic prediction showed that there were significant differences in the phenotypes of amino acid, glucose, and lipid metabolism of gut microbiota in the NAFLD group (P < .05). receiver operating characteristic curve analysis revealed that combination of Bacteroides and the BF ratio resulted in 88% and 100% sensitivity and specificity, respectively, when used for NAFLD diagnosis. Metabolomics and bioinformatics analysis revealed changes in the metabolism of nicotinate, nicotinamide, and pyrimidine; signaling pathways of calcium and oxytocin; pancreatic secretion with metabolites such as uracil, xanthine, and biliverdin; and enzymes such as xanthine dehydrogenase and xanthine oxidase (P < .05). Therefore, the phenotypic changes may be a potential marker for NAFLD and we considered that a combined analysis of Bacteroides and BF ratio had good diagnostic accuracy for NAFLD.http://journals.lww.com/10.1097/MD.0000000000029347 |
| spellingShingle | Haize Ge, PhD Wei Wei, MD Liang Tang, PhD Yaqiong Tian, BD Yu Zhu, PhD Yan Luo, MD Shuye Liu, BD CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients Medicine |
| title | CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients |
| title_full | CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients |
| title_fullStr | CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients |
| title_full_unstemmed | CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients |
| title_short | CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients |
| title_sort | consort characteristics and metabolic phenotype of gut microbiota in nafld patients |
| url | http://journals.lww.com/10.1097/MD.0000000000029347 |
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