Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i>
Metabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from ar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/5/588 |
| _version_ | 1797599102345674752 |
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| author | Diego Armando Badillo-Sanchez Donald J. L. Jones Sarah A. Inskip Christiana L. Scheib |
| author_facet | Diego Armando Badillo-Sanchez Donald J. L. Jones Sarah A. Inskip Christiana L. Scheib |
| author_sort | Diego Armando Badillo-Sanchez |
| collection | DOAJ |
| description | Metabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from archaeological human dentin. Dentin obtained from micro sampling the dental pulp of teeth of victims and non-victims of <i>Yersinia pestis</i> (plague) from a 6th century Cambridgeshire site are used to evaluate the potential use of such unique material for untargeted metabolomic studies on disease state through liquid chromatography hyphenated to high-resolution mass spectrometry (LC-HRMS). Results show that small molecules of both likely endogenous and exogenous sources are preserved for a range of polar and less polar/apolar metabolites in archaeological dentin; however, untargeted metabolomic profiles show no clear differentiation between healthy and infected individuals in the small sample analysed (<i>n</i> = 20). This study discusses the potential of dentin as a source of small molecules for metabolomic assays and highlights: (1) the need for follow up research to optimise sampling protocols, (2) the requirements of studies with larger sample numbers and (3) the necessity of more databases to amplify the positive results achievable with this Omic technique in the archaeological sciences. |
| first_indexed | 2024-03-11T03:29:59Z |
| format | Article |
| id | doaj.art-0ea9dd8d40c849278b4da56c866b7afa |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T03:29:59Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0ea9dd8d40c849278b4da56c866b7afa2023-11-18T02:24:59ZengMDPI AGMetabolites2218-19892023-04-0113558810.3390/metabo13050588Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i>Diego Armando Badillo-Sanchez0Donald J. L. Jones1Sarah A. Inskip2Christiana L. Scheib3School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UKLeicester Cancer Research Centre, RKCSB, University of Leicester, Leicester LE1 7RH, UKSchool of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UKInstitute of Genomics, University of Tartu, 51010 Tartu, EstoniaMetabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from archaeological human dentin. Dentin obtained from micro sampling the dental pulp of teeth of victims and non-victims of <i>Yersinia pestis</i> (plague) from a 6th century Cambridgeshire site are used to evaluate the potential use of such unique material for untargeted metabolomic studies on disease state through liquid chromatography hyphenated to high-resolution mass spectrometry (LC-HRMS). Results show that small molecules of both likely endogenous and exogenous sources are preserved for a range of polar and less polar/apolar metabolites in archaeological dentin; however, untargeted metabolomic profiles show no clear differentiation between healthy and infected individuals in the small sample analysed (<i>n</i> = 20). This study discusses the potential of dentin as a source of small molecules for metabolomic assays and highlights: (1) the need for follow up research to optimise sampling protocols, (2) the requirements of studies with larger sample numbers and (3) the necessity of more databases to amplify the positive results achievable with this Omic technique in the archaeological sciences.https://www.mdpi.com/2218-1989/13/5/588untargeted metabolomicsLC-HRMShuman dentinbiomolecular archaeologydiseaseplague |
| spellingShingle | Diego Armando Badillo-Sanchez Donald J. L. Jones Sarah A. Inskip Christiana L. Scheib Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> Metabolites untargeted metabolomics LC-HRMS human dentin biomolecular archaeology disease plague |
| title | Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> |
| title_full | Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> |
| title_fullStr | Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> |
| title_full_unstemmed | Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> |
| title_short | Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of <i>Yersinia pestis</i> |
| title_sort | human archaeological dentin as source of polar and less polar metabolites for untargeted metabolomic research the case of i yersinia pestis i |
| topic | untargeted metabolomics LC-HRMS human dentin biomolecular archaeology disease plague |
| url | https://www.mdpi.com/2218-1989/13/5/588 |
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