Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury
Central nervous system (CNS) function recovery following stroke remains a major challenge because neural regeneration is difficult to achieve. In this study, rigid-flexible composite scaffolds consisting of nanofibers from electrospun scaffolds and self-adapting and injectable hydrogel were loaded w...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Materials Today Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590049823000097 |
| _version_ | 1811160790037692416 |
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| author | Yanhong Pei Lifei Huang Tong Wang Qinhan Yao Yanrong Sun Yan Zhang Xiaomei Yang Jiliang Zhai Lihua Qin Jiajia Xue Xing Wang Hongquan Zhang Junhao Yan |
| author_facet | Yanhong Pei Lifei Huang Tong Wang Qinhan Yao Yanrong Sun Yan Zhang Xiaomei Yang Jiliang Zhai Lihua Qin Jiajia Xue Xing Wang Hongquan Zhang Junhao Yan |
| author_sort | Yanhong Pei |
| collection | DOAJ |
| description | Central nervous system (CNS) function recovery following stroke remains a major challenge because neural regeneration is difficult to achieve. In this study, rigid-flexible composite scaffolds consisting of nanofibers from electrospun scaffolds and self-adapting and injectable hydrogel were loaded with bone marrow mesenchymal stem cells (BMSCs), and the effects of these loaded BMSCs on ischemic insult were investigated. In vitro analysis of the viability, migration, neurite growth, angiogenic capacity, and paracrine effects of BMSCs indicated that BMSCs loaded in composite scaffolds had a better therapeutic effect than those BMSCs in saline. Furthermore, in vivo, BMSCs loaded in composite scaffolds significantly reduced the extent of brain edema and the infarct volume, alleviated neurological deficits, markedly attenuated microglial and astrocyte overactivation, and increased neuronal proliferation and vascular growth. Bioinformatics analysis revealed that BMSCs loaded in composite scaffolds could decrease the level of exosomal miR-206–3p and consequently increase the activity of the PI3K/AKT signaling pathway. In conclusion, BMSCs loaded in novel composite scaffolds exert obvious neuroprotective effects, attenuating ischemic injury by enhancing angiogenesis and neural regeneration in the brain after ischemic stroke, and these results provide a promising approach for treating CNS diseases in the clinic via cell transplantation. |
| first_indexed | 2024-04-10T06:04:34Z |
| format | Article |
| id | doaj.art-0eaa5d8573cf40e78d7ab032b55167ca |
| institution | Directory Open Access Journal |
| issn | 2590-0498 |
| language | English |
| last_indexed | 2024-04-10T06:04:34Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Advances |
| spelling | doaj.art-0eaa5d8573cf40e78d7ab032b55167ca2023-03-03T04:25:15ZengElsevierMaterials Today Advances2590-04982023-03-0117100349Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injuryYanhong Pei0Lifei Huang1Tong Wang2Qinhan Yao3Yanrong Sun4Yan Zhang5Xiaomei Yang6Jiliang Zhai7Lihua Qin8Jiajia Xue9Xing Wang10Hongquan Zhang11Junhao Yan12Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China; Program for Cancer and Cell Biology, Department of Human Anatomy, Histology and Embryology, PKU International Cancer Institute, MOE Key Laboratory of Carcinogenesis and Translational Research and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing, 100191, ChinaState Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, ChinaState Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, ChinaDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, ChinaDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, ChinaDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, ChinaDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, ChinaDepartment of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, ChinaState Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China; Corresponding author.State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China; Corresponding authors.Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China; Program for Cancer and Cell Biology, Department of Human Anatomy, Histology and Embryology, PKU International Cancer Institute, MOE Key Laboratory of Carcinogenesis and Translational Research and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing, 100191, China; Corresponding author. Program for Cancer and Cell Biology, Department of Human Anatomy, Histology and Embryology, PKU International Cancer Institute, MOE Key Laboratory of Carcinogenesis and Translational Research and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing, China.Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China; Beijing Key Lab of Magnetic Resonance Imaging Technology, Peking University Third Hospital, Beijing, 100191, China; Corresponding author. Department of Anatomy, Histology and Embryology, School of Basic Medical Science, Peking University Health Science Center, No.38 Xueyuan Road, Haidian District, Beijing 100191, China.Central nervous system (CNS) function recovery following stroke remains a major challenge because neural regeneration is difficult to achieve. In this study, rigid-flexible composite scaffolds consisting of nanofibers from electrospun scaffolds and self-adapting and injectable hydrogel were loaded with bone marrow mesenchymal stem cells (BMSCs), and the effects of these loaded BMSCs on ischemic insult were investigated. In vitro analysis of the viability, migration, neurite growth, angiogenic capacity, and paracrine effects of BMSCs indicated that BMSCs loaded in composite scaffolds had a better therapeutic effect than those BMSCs in saline. Furthermore, in vivo, BMSCs loaded in composite scaffolds significantly reduced the extent of brain edema and the infarct volume, alleviated neurological deficits, markedly attenuated microglial and astrocyte overactivation, and increased neuronal proliferation and vascular growth. Bioinformatics analysis revealed that BMSCs loaded in composite scaffolds could decrease the level of exosomal miR-206–3p and consequently increase the activity of the PI3K/AKT signaling pathway. In conclusion, BMSCs loaded in novel composite scaffolds exert obvious neuroprotective effects, attenuating ischemic injury by enhancing angiogenesis and neural regeneration in the brain after ischemic stroke, and these results provide a promising approach for treating CNS diseases in the clinic via cell transplantation.http://www.sciencedirect.com/science/article/pii/S2590049823000097Bone marrow mesenchymal stem cellsExosomeHydrogelNanofiberIschemic stroke |
| spellingShingle | Yanhong Pei Lifei Huang Tong Wang Qinhan Yao Yanrong Sun Yan Zhang Xiaomei Yang Jiliang Zhai Lihua Qin Jiajia Xue Xing Wang Hongquan Zhang Junhao Yan Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury Materials Today Advances Bone marrow mesenchymal stem cells Exosome Hydrogel Nanofiber Ischemic stroke |
| title | Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury |
| title_full | Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury |
| title_fullStr | Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury |
| title_full_unstemmed | Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury |
| title_short | Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury |
| title_sort | bone marrow mesenchymal stem cells loaded into hydrogel nanofiber composite scaffolds ameliorate ischemic brain injury |
| topic | Bone marrow mesenchymal stem cells Exosome Hydrogel Nanofiber Ischemic stroke |
| url | http://www.sciencedirect.com/science/article/pii/S2590049823000097 |
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