Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia.
<h4>Background</h4>Pteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. T...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-09-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0009768 |
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| author | Hayato Harima Michihito Sasaki Yasuko Orba Kosuke Okuya Yongjin Qiu Christida E Wastika Katendi Changula Masahiro Kajihara Edgar Simulundu Tomoyuki Yamaguchi Yoshiki Eto Akina Mori-Kajihara Akihiko Sato Satoshi Taniguchi Ayato Takada Masayuki Saijo Bernard M Hang'ombe Hirofumi Sawa |
| author_facet | Hayato Harima Michihito Sasaki Yasuko Orba Kosuke Okuya Yongjin Qiu Christida E Wastika Katendi Changula Masahiro Kajihara Edgar Simulundu Tomoyuki Yamaguchi Yoshiki Eto Akina Mori-Kajihara Akihiko Sato Satoshi Taniguchi Ayato Takada Masayuki Saijo Bernard M Hang'ombe Hirofumi Sawa |
| author_sort | Hayato Harima |
| collection | DOAJ |
| description | <h4>Background</h4>Pteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. Therefore, this study performed an PRV surveillance in fruit bats in Zambia.<h4>Methods</h4>Egyptian fruit bats (Rousettus aegyptiacus, n = 47) and straw-colored fruit bats (Eidolon helvum, n = 33) captured in Zambia in 2017-2018 were screened for PRV infection using RT-PCR and serum neutralization tests. The complete genome sequence of an isolated PRV strain was determined by next generation sequencing and subjected to BLAST and phylogenetic analyses. Replication capacity and pathogenicity of the strain were investigated using Vero E6 cell cultures and BALB/c mice, respectively.<h4>Results</h4>An PRV strain, tentatively named Nachunsulwe-57, was isolated from one Egyptian fruit bat. Serological assays demonstrated that 98% of sera (69/70) collected from Egyptian fruit bats (n = 37) and straw-colored fruit bats (n = 33) had neutralizing antibodies against PRV. Genetic analyses revealed that all 10 genome segments of Nachunsulwe-57 were closely related to a bat-derived Kasama strain found in Uganda. Nachunsulwe-57 showed less efficiency in viral growth and lower pathogenicity in mice than another PRV strain, Miyazaki-Bali/2007, isolated from a patient.<h4>Conclusions</h4>A high proportion of Egyptian fruit bats and straw-colored fruit bats were found to be seropositive to PRV in Zambia. Importantly, a new PRV strain (Nachunsulwe-57) was isolated from an Egyptian fruit bat in Zambia, which had relatively weak pathogenicity in mice. Taken together, our findings provide new epidemiological insights about PRV infection in bats and indicate the first isolation of an PRV strain that may have low pathogenicity to humans. |
| first_indexed | 2024-12-13T21:39:19Z |
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| id | doaj.art-0eac1e1367d64eb8ac15fd685e9c3f21 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-13T21:39:19Z |
| publishDate | 2021-09-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-0eac1e1367d64eb8ac15fd685e9c3f212022-12-21T23:30:35ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-09-01159e000976810.1371/journal.pntd.0009768Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia.Hayato HarimaMichihito SasakiYasuko OrbaKosuke OkuyaYongjin QiuChristida E WastikaKatendi ChangulaMasahiro KajiharaEdgar SimulunduTomoyuki YamaguchiYoshiki EtoAkina Mori-KajiharaAkihiko SatoSatoshi TaniguchiAyato TakadaMasayuki SaijoBernard M Hang'ombeHirofumi Sawa<h4>Background</h4>Pteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. Therefore, this study performed an PRV surveillance in fruit bats in Zambia.<h4>Methods</h4>Egyptian fruit bats (Rousettus aegyptiacus, n = 47) and straw-colored fruit bats (Eidolon helvum, n = 33) captured in Zambia in 2017-2018 were screened for PRV infection using RT-PCR and serum neutralization tests. The complete genome sequence of an isolated PRV strain was determined by next generation sequencing and subjected to BLAST and phylogenetic analyses. Replication capacity and pathogenicity of the strain were investigated using Vero E6 cell cultures and BALB/c mice, respectively.<h4>Results</h4>An PRV strain, tentatively named Nachunsulwe-57, was isolated from one Egyptian fruit bat. Serological assays demonstrated that 98% of sera (69/70) collected from Egyptian fruit bats (n = 37) and straw-colored fruit bats (n = 33) had neutralizing antibodies against PRV. Genetic analyses revealed that all 10 genome segments of Nachunsulwe-57 were closely related to a bat-derived Kasama strain found in Uganda. Nachunsulwe-57 showed less efficiency in viral growth and lower pathogenicity in mice than another PRV strain, Miyazaki-Bali/2007, isolated from a patient.<h4>Conclusions</h4>A high proportion of Egyptian fruit bats and straw-colored fruit bats were found to be seropositive to PRV in Zambia. Importantly, a new PRV strain (Nachunsulwe-57) was isolated from an Egyptian fruit bat in Zambia, which had relatively weak pathogenicity in mice. Taken together, our findings provide new epidemiological insights about PRV infection in bats and indicate the first isolation of an PRV strain that may have low pathogenicity to humans.https://doi.org/10.1371/journal.pntd.0009768 |
| spellingShingle | Hayato Harima Michihito Sasaki Yasuko Orba Kosuke Okuya Yongjin Qiu Christida E Wastika Katendi Changula Masahiro Kajihara Edgar Simulundu Tomoyuki Yamaguchi Yoshiki Eto Akina Mori-Kajihara Akihiko Sato Satoshi Taniguchi Ayato Takada Masayuki Saijo Bernard M Hang'ombe Hirofumi Sawa Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. PLoS Neglected Tropical Diseases |
| title | Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. |
| title_full | Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. |
| title_fullStr | Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. |
| title_full_unstemmed | Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. |
| title_short | Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia. |
| title_sort | attenuated infection by a pteropine orthoreovirus isolated from an egyptian fruit bat in zambia |
| url | https://doi.org/10.1371/journal.pntd.0009768 |
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