Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces
Abstract In the absence of liquid suspension, dry biofilms can form upon hard surfaces within a hospital environment, representing a healthcare‐associated infection risk. Probiotic cleansers using generally recognized as safe organisms, such as those of the Bacillus genus, represent a potential stra...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.1391 |
| _version_ | 1797382756886380544 |
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| author | Richard Wormald Paul N. Humphreys Christopher J. Charles Simon P. Rout |
| author_facet | Richard Wormald Paul N. Humphreys Christopher J. Charles Simon P. Rout |
| author_sort | Richard Wormald |
| collection | DOAJ |
| description | Abstract In the absence of liquid suspension, dry biofilms can form upon hard surfaces within a hospital environment, representing a healthcare‐associated infection risk. Probiotic cleansers using generally recognized as safe organisms, such as those of the Bacillus genus, represent a potential strategy for the reduction of dry biofilm bioburden. The mechanisms of action and efficacy of these cleaners are, however, poorly understood. To address this, a preventative dry biofilm assay was developed using steel, melamine, and ceramic surfaces to assess the ability of a commercially available Bacillus spp. based probiotic cleanser to reduce the surface bioburden of Escherichia coli and Staphylococcus aureus. Via this assay, phosphate‐buffered saline controls were able to generate dry biofilms within 7 days of incubation, with the application of the probiotic cleanser able to prevent >97.7% of dry biofilm formation across both pathogen analogs and surface types. Further to this, surfaces treated with the probiotic mixture alone also showed a reduction in dry biofilm across both pathogen and surface types. Confocal laser scanning microscopy imaging indicated that the probiotic bacteria were able to germinate and colonize surfaces, likely forming a protective layer upon these hard surfaces. |
| first_indexed | 2024-03-08T21:10:05Z |
| format | Article |
| id | doaj.art-0eaca843817c4747a317d4a6e7f89004 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-03-08T21:10:05Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-0eaca843817c4747a317d4a6e7f890042023-12-22T05:36:29ZengWileyMicrobiologyOpen2045-88272023-12-01126n/an/a10.1002/mbo3.1391Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfacesRichard Wormald0Paul N. Humphreys1Christopher J. Charles2Simon P. Rout3Department of Biological and Geographical Sciences University of Huddersfield Huddersfield UKDepartment of Biological and Geographical Sciences University of Huddersfield Huddersfield UKGenesis Biosciences Cardiff UKDepartment of Biological and Geographical Sciences University of Huddersfield Huddersfield UKAbstract In the absence of liquid suspension, dry biofilms can form upon hard surfaces within a hospital environment, representing a healthcare‐associated infection risk. Probiotic cleansers using generally recognized as safe organisms, such as those of the Bacillus genus, represent a potential strategy for the reduction of dry biofilm bioburden. The mechanisms of action and efficacy of these cleaners are, however, poorly understood. To address this, a preventative dry biofilm assay was developed using steel, melamine, and ceramic surfaces to assess the ability of a commercially available Bacillus spp. based probiotic cleanser to reduce the surface bioburden of Escherichia coli and Staphylococcus aureus. Via this assay, phosphate‐buffered saline controls were able to generate dry biofilms within 7 days of incubation, with the application of the probiotic cleanser able to prevent >97.7% of dry biofilm formation across both pathogen analogs and surface types. Further to this, surfaces treated with the probiotic mixture alone also showed a reduction in dry biofilm across both pathogen and surface types. Confocal laser scanning microscopy imaging indicated that the probiotic bacteria were able to germinate and colonize surfaces, likely forming a protective layer upon these hard surfaces.https://doi.org/10.1002/mbo3.1391BacillusbiofilmCLSM‐FISHprobioticsurface |
| spellingShingle | Richard Wormald Paul N. Humphreys Christopher J. Charles Simon P. Rout Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces MicrobiologyOpen Bacillus biofilm CLSM‐FISH probiotic surface |
| title | Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| title_full | Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| title_fullStr | Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| title_full_unstemmed | Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| title_short | Bacillus‐based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| title_sort | bacillus based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces |
| topic | Bacillus biofilm CLSM‐FISH probiotic surface |
| url | https://doi.org/10.1002/mbo3.1391 |
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