Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome
Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under condit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-06-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Gubskyi_3_14.pdf |
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| author | Yu. I. Gubskyi T. A. Buhtiarova H. G. Goriushko N. V. Litvinova G. I. Paramonova T. N. Kurapova O. N. Velychko L. P. Babenko |
| author_facet | Yu. I. Gubskyi T. A. Buhtiarova H. G. Goriushko N. V. Litvinova G. I. Paramonova T. N. Kurapova O. N. Velychko L. P. Babenko |
| author_sort | Yu. I. Gubskyi |
| collection | DOAJ |
| description | Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration. |
| first_indexed | 2024-03-09T08:49:37Z |
| format | Article |
| id | doaj.art-0eae0cb94cfc467a99381613c4d3db85 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:49:37Z |
| publishDate | 2014-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-0eae0cb94cfc467a99381613c4d3db852023-12-02T14:35:55ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032014-06-018639810610.15407/ubj86.03.098Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndromeYu. I. Gubskyi0T. A. Buhtiarova1H. G. Goriushko2N. V. Litvinova3G. I. Paramonova4T. N. Kurapova5O. N. Velychko6L. P. Babenko7SI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivSI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, KyivMethods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Gubskyi_3_14.pdfacute nociceptive painerythrocytes membranesfluorescent probingmicrocalorimetrynon-opioid analgesicsoxidative stress |
| spellingShingle | Yu. I. Gubskyi T. A. Buhtiarova H. G. Goriushko N. V. Litvinova G. I. Paramonova T. N. Kurapova O. N. Velychko L. P. Babenko Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome The Ukrainian Biochemical Journal acute nociceptive pain erythrocytes membranes fluorescent probing microcalorimetry non-opioid analgesics oxidative stress |
| title | Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome |
| title_full | Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome |
| title_fullStr | Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome |
| title_full_unstemmed | Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome |
| title_short | Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome |
| title_sort | biophysical parameters of erythrocyte membranes and mechanisms of interaction with non opioid analgesics under acute pain syndrome |
| topic | acute nociceptive pain erythrocytes membranes fluorescent probing microcalorimetry non-opioid analgesics oxidative stress |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Gubskyi_3_14.pdf |
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