Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
Plant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1167839/full |
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| author | Esaú De la Vega-Camarillo Josimar Sotelo-Aguilar Bibiana Rios-Galicia Yuridia Mercado-Flores Ramón Arteaga-Garibay Lourdes Villa-Tanaca César Hernández-Rodríguez |
| author_facet | Esaú De la Vega-Camarillo Josimar Sotelo-Aguilar Bibiana Rios-Galicia Yuridia Mercado-Flores Ramón Arteaga-Garibay Lourdes Villa-Tanaca César Hernández-Rodríguez |
| author_sort | Esaú De la Vega-Camarillo |
| collection | DOAJ |
| description | Plant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communities (SynCom) were assembled with a set of PGPB isolated from the Jala maize landrace, some of them with additional abilities for the biocontrol of phytopathogenic fungi and the stimulation of plant-induced systemic resistance (ISR). Three SynCom were designed considering the phenotypic traits of bacterial strains, including Achromobacter xylosoxidans Z2K8, Burkholderia sp. Z1AL11, Klebsiella variicola R3J3HD7, Kosakonia pseudosacchari Z2WD1, Pantoea ananatis E2HD8, Pantoea sp. E2AD2, Phytobacter diazotrophicus Z2WL1, Pseudomonas protegens E1BL2, and P. protegens E2HL9. Plant growth promotion in gnotobiotic and greenhouse seedlings assays was performed with Conejo landrace; meanwhile, open field tests were carried out on hybrid CPL9105W maize. In all experimental models, a significant promotion of plant growth was observed. In gnotobiotic assays, the roots and shoot length of the maize seedlings increased 4.2 and 3.0 times, respectively, compared to the untreated control. Similarly, the sizes and weights of the roots and shoots of the plants increased significantly in the greenhouse assays. In the open field assay performed with hybrid CPL9105W maize, the yield increased from 11 tons/ha for the control to 16 tons/ha inoculated with SynCom 3. In addition, the incidence of rust fungal infections decreased significantly from 12.5% in the control to 8% in the treatment with SynCom 3. All SynCom designs promoted the growth of maize in all assays. However, SynCom 3 formulated with A. xylosoxidans Z2K8, Burkholderia sp. Z1AL11, K. variicola R3J3HD7, P. ananatis E2HD8, P. diazotrophicus Z2WL1, and P. protegens E1BL2 displayed the best results for promoting plant growth, their yield, and the inhibition of fungal rust. This study demonstrated the biotechnological eco-friendly plant growth-promoting potential of SynCom assemblies with culturable bacteria from native maize landraces for more sustainable and economic agriculture. |
| first_indexed | 2024-03-13T10:26:27Z |
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| id | doaj.art-0eae6d5ad7244fe1a8a9945cdbc800a4 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-13T10:26:27Z |
| publishDate | 2023-05-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-0eae6d5ad7244fe1a8a9945cdbc800a42023-05-19T05:43:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11678391167839Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maizeEsaú De la Vega-Camarillo0Josimar Sotelo-Aguilar1Bibiana Rios-Galicia2Yuridia Mercado-Flores3Ramón Arteaga-Garibay4Lourdes Villa-Tanaca5César Hernández-Rodríguez6Laboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoLaboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoLaboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoLaboratorio de Aprovechamiento Integral de Recursos Bióticos, Universidad Politécnica de Pachuca, Hidalgo, MexicoLaboratorio de Recursos Genéticos Microbianos, Centro Nacional de Recursos Genéticos, INIFAP, Jalisco, MexicoLaboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoLaboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoPlant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communities (SynCom) were assembled with a set of PGPB isolated from the Jala maize landrace, some of them with additional abilities for the biocontrol of phytopathogenic fungi and the stimulation of plant-induced systemic resistance (ISR). Three SynCom were designed considering the phenotypic traits of bacterial strains, including Achromobacter xylosoxidans Z2K8, Burkholderia sp. Z1AL11, Klebsiella variicola R3J3HD7, Kosakonia pseudosacchari Z2WD1, Pantoea ananatis E2HD8, Pantoea sp. E2AD2, Phytobacter diazotrophicus Z2WL1, Pseudomonas protegens E1BL2, and P. protegens E2HL9. Plant growth promotion in gnotobiotic and greenhouse seedlings assays was performed with Conejo landrace; meanwhile, open field tests were carried out on hybrid CPL9105W maize. In all experimental models, a significant promotion of plant growth was observed. In gnotobiotic assays, the roots and shoot length of the maize seedlings increased 4.2 and 3.0 times, respectively, compared to the untreated control. Similarly, the sizes and weights of the roots and shoots of the plants increased significantly in the greenhouse assays. In the open field assay performed with hybrid CPL9105W maize, the yield increased from 11 tons/ha for the control to 16 tons/ha inoculated with SynCom 3. In addition, the incidence of rust fungal infections decreased significantly from 12.5% in the control to 8% in the treatment with SynCom 3. All SynCom designs promoted the growth of maize in all assays. However, SynCom 3 formulated with A. xylosoxidans Z2K8, Burkholderia sp. Z1AL11, K. variicola R3J3HD7, P. ananatis E2HD8, P. diazotrophicus Z2WL1, and P. protegens E1BL2 displayed the best results for promoting plant growth, their yield, and the inhibition of fungal rust. This study demonstrated the biotechnological eco-friendly plant growth-promoting potential of SynCom assemblies with culturable bacteria from native maize landraces for more sustainable and economic agriculture.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1167839/fullendophytic bacteriaplant growth-promoting bacteria (PGPB)Jala maizesynthetic microbial communities (SynCom)plant-microbe interactioninduced systemic resistance (ISR) |
| spellingShingle | Esaú De la Vega-Camarillo Josimar Sotelo-Aguilar Bibiana Rios-Galicia Yuridia Mercado-Flores Ramón Arteaga-Garibay Lourdes Villa-Tanaca César Hernández-Rodríguez Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize Frontiers in Microbiology endophytic bacteria plant growth-promoting bacteria (PGPB) Jala maize synthetic microbial communities (SynCom) plant-microbe interaction induced systemic resistance (ISR) |
| title | Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize |
| title_full | Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize |
| title_fullStr | Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize |
| title_full_unstemmed | Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize |
| title_short | Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize |
| title_sort | promotion of the growth and yield of zea mays by synthetic microbial communities from jala maize |
| topic | endophytic bacteria plant growth-promoting bacteria (PGPB) Jala maize synthetic microbial communities (SynCom) plant-microbe interaction induced systemic resistance (ISR) |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1167839/full |
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