Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon
Damping-off caused by <i>Pythium aphanidermatum</i> (<i>Pa</i>) is one of the most destructive diseases for watermelon seedlings. Application of biological control agents against <i>Pa</i> has attracted the attention of many researchers for a long time. In this st...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1360 |
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| author | Mihong Ge Xiang Cai Dehuan Wang Huan Liang Juhong Zhu Guoqing Li Xianfeng Shi |
| author_facet | Mihong Ge Xiang Cai Dehuan Wang Huan Liang Juhong Zhu Guoqing Li Xianfeng Shi |
| author_sort | Mihong Ge |
| collection | DOAJ |
| description | Damping-off caused by <i>Pythium aphanidermatum</i> (<i>Pa</i>) is one of the most destructive diseases for watermelon seedlings. Application of biological control agents against <i>Pa</i> has attracted the attention of many researchers for a long time. In this study, the actinomycetous isolate JKTJ-3 with strong and broad-spectrum antifungal activity was screened from 23 bacterial isolates. Based on the morphological, cultural, physiological, and biochemical characteristics as well as the feature of 16S rDNA sequence, isolate JKTJ-3 was identified as <i>Streptomyces murinus</i>. We investigated the biocontrol efficacy of isolate JKTJ-3 and its metabolites. The results revealed that seed and substrate treatments with JKTJ-3 cultures showed a significant inhibitory effect on watermelon damping-off disease. Seed treatment with the JKTJ-3 cultural filtrates (CF) displayed higher control efficacy compared to the fermentation cultures (FC). Treatment of the seeding substrate with the wheat grain cultures (WGC) of JKTJ-3 exhibited better control efficacy than that of the seeding substrate with the JKTJ-3 CF. Moreover, the JKTJ-3 WGC showed the preventive effect on suppression of the disease, and the efficacy increased with increase in the inoculation interval between the WGC and <i>Pa</i>. Production of the antifungal metabolite actinomycin D by isolate JKTJ-3 and cell-wall-degrading enzymes such as β-1,3-glucanase and chitosanase were probably the mechanisms for effective control of watermelon damping-off. It was shown for the first time that <i>S. murinus</i> can produce anti-oomycete substances including chitinase and actinomycin D. This is the first report about <i>S. murinus</i> used as biocontrol agent against watermelon damping-off caused by <i>Pa</i>. |
| first_indexed | 2024-03-11T02:07:54Z |
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| language | English |
| last_indexed | 2024-03-11T02:07:54Z |
| publishDate | 2023-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-0eae9d5477c444de94043253730a15692023-11-18T11:40:51ZengMDPI AGMicroorganisms2076-26072023-05-01116136010.3390/microorganisms11061360Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of WatermelonMihong Ge0Xiang Cai1Dehuan Wang2Huan Liang3Juhong Zhu4Guoqing Li5Xianfeng Shi6State Key Laboratory of Agricultural Microbiology, Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430070, ChinaDamping-off caused by <i>Pythium aphanidermatum</i> (<i>Pa</i>) is one of the most destructive diseases for watermelon seedlings. Application of biological control agents against <i>Pa</i> has attracted the attention of many researchers for a long time. In this study, the actinomycetous isolate JKTJ-3 with strong and broad-spectrum antifungal activity was screened from 23 bacterial isolates. Based on the morphological, cultural, physiological, and biochemical characteristics as well as the feature of 16S rDNA sequence, isolate JKTJ-3 was identified as <i>Streptomyces murinus</i>. We investigated the biocontrol efficacy of isolate JKTJ-3 and its metabolites. The results revealed that seed and substrate treatments with JKTJ-3 cultures showed a significant inhibitory effect on watermelon damping-off disease. Seed treatment with the JKTJ-3 cultural filtrates (CF) displayed higher control efficacy compared to the fermentation cultures (FC). Treatment of the seeding substrate with the wheat grain cultures (WGC) of JKTJ-3 exhibited better control efficacy than that of the seeding substrate with the JKTJ-3 CF. Moreover, the JKTJ-3 WGC showed the preventive effect on suppression of the disease, and the efficacy increased with increase in the inoculation interval between the WGC and <i>Pa</i>. Production of the antifungal metabolite actinomycin D by isolate JKTJ-3 and cell-wall-degrading enzymes such as β-1,3-glucanase and chitosanase were probably the mechanisms for effective control of watermelon damping-off. It was shown for the first time that <i>S. murinus</i> can produce anti-oomycete substances including chitinase and actinomycin D. This is the first report about <i>S. murinus</i> used as biocontrol agent against watermelon damping-off caused by <i>Pa</i>.https://www.mdpi.com/2076-2607/11/6/1360watermelondamping-off<i>Pythium aphanidermatum</i><i>Streptomyces murinus</i>biocontrol |
| spellingShingle | Mihong Ge Xiang Cai Dehuan Wang Huan Liang Juhong Zhu Guoqing Li Xianfeng Shi Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon Microorganisms watermelon damping-off <i>Pythium aphanidermatum</i> <i>Streptomyces murinus</i> biocontrol |
| title | Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon |
| title_full | Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon |
| title_fullStr | Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon |
| title_full_unstemmed | Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon |
| title_short | Efficacy of <i>Streptomyces murinus</i> JKTJ-3 in Suppression of <i>Pythium</i> Damping-Off of Watermelon |
| title_sort | efficacy of i streptomyces murinus i jktj 3 in suppression of i pythium i damping off of watermelon |
| topic | watermelon damping-off <i>Pythium aphanidermatum</i> <i>Streptomyces murinus</i> biocontrol |
| url | https://www.mdpi.com/2076-2607/11/6/1360 |
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