Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats
Abstract The objective of the current animal study was to investigate factors contributing to the different phases of the cystometrogram (CMG) in order to address disparities in research data reported in the current literature. Three experiments in 20 female Wistar rats were designed to investigate...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14724 |
| _version_ | 1818573914393018368 |
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| author | Daniel Medina‐Aguinaga Robert F. Hoey Alvaro Munoz Moises Altamira‐Camacho Jose L. Quintanar Charles H. Hubscher |
| author_facet | Daniel Medina‐Aguinaga Robert F. Hoey Alvaro Munoz Moises Altamira‐Camacho Jose L. Quintanar Charles H. Hubscher |
| author_sort | Daniel Medina‐Aguinaga |
| collection | DOAJ |
| description | Abstract The objective of the current animal study was to investigate factors contributing to the different phases of the cystometrogram (CMG) in order to address disparities in research data reported in the current literature. Three experiments in 20 female Wistar rats were designed to investigate (1) the effects of anesthesia on the contractile pattern of the bladder during micturition; (2) the impact of the physical characteristics of the CMG technique upon the accuracy of intra‐vesical pressure recordings; and (3) identification of physiological and methodological factors associated with the emptying and rebound phases during CMG. Variables tested included awake versus urethane‐anesthetized conditions, use of a single catheter for both filling and intra‐vesical pressure (Pves) recording versus a separate two catheter approach, and comparisons between ureter, bladder dome, and urethral catheter placements. Both awake and anesthetized conditions contributed to variations in the shape and magnitude of the CMG pressure curves. In addition, catheter size, acute incision of the bladder dome for catheter placement, use of the same catheter for filling and Pves recordings, as well as the placement and positioning of the tubing, all contributed to alterations of the physiological properties and characteristic of the various CMG phases, including the frequent occurrence of an artificial rebound during the third phase of micturition. The present results demonstrate how different experimental conditions lead not only to variability in Pves curves, but consistency of the measurements as well, which needs to be accounted for when interpreting CMG outcome data. |
| first_indexed | 2024-12-15T00:19:17Z |
| format | Article |
| id | doaj.art-0eaf1dbfce5840189171e9ebe945a8c6 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-15T00:19:17Z |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-0eaf1dbfce5840189171e9ebe945a8c62022-12-21T22:42:22ZengWileyPhysiological Reports2051-817X2021-01-0192n/an/a10.14814/phy2.14724Choice of cystometric technique impacts detrusor contractile dynamics in wistar ratsDaniel Medina‐Aguinaga0Robert F. Hoey1Alvaro Munoz2Moises Altamira‐Camacho3Jose L. Quintanar4Charles H. Hubscher5Department of Anatomical Sciences & Neurobiology University of Louisville Louisville KY USADepartment of Anatomical Sciences & Neurobiology University of Louisville Louisville KY USADepartment of Foundations of Knowledge Centro Universitario del NorteUniversity of Guadalajara Colotlan MexicoDepartment of Physiology & Pharmacology UAA Aguascalientes MexicoDepartment of Physiology & Pharmacology UAA Aguascalientes MexicoDepartment of Anatomical Sciences & Neurobiology University of Louisville Louisville KY USAAbstract The objective of the current animal study was to investigate factors contributing to the different phases of the cystometrogram (CMG) in order to address disparities in research data reported in the current literature. Three experiments in 20 female Wistar rats were designed to investigate (1) the effects of anesthesia on the contractile pattern of the bladder during micturition; (2) the impact of the physical characteristics of the CMG technique upon the accuracy of intra‐vesical pressure recordings; and (3) identification of physiological and methodological factors associated with the emptying and rebound phases during CMG. Variables tested included awake versus urethane‐anesthetized conditions, use of a single catheter for both filling and intra‐vesical pressure (Pves) recording versus a separate two catheter approach, and comparisons between ureter, bladder dome, and urethral catheter placements. Both awake and anesthetized conditions contributed to variations in the shape and magnitude of the CMG pressure curves. In addition, catheter size, acute incision of the bladder dome for catheter placement, use of the same catheter for filling and Pves recordings, as well as the placement and positioning of the tubing, all contributed to alterations of the physiological properties and characteristic of the various CMG phases, including the frequent occurrence of an artificial rebound during the third phase of micturition. The present results demonstrate how different experimental conditions lead not only to variability in Pves curves, but consistency of the measurements as well, which needs to be accounted for when interpreting CMG outcome data.https://doi.org/10.14814/phy2.14724bladdercystometrogramexternal urethral sphinctermicturitionurodynamics |
| spellingShingle | Daniel Medina‐Aguinaga Robert F. Hoey Alvaro Munoz Moises Altamira‐Camacho Jose L. Quintanar Charles H. Hubscher Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats Physiological Reports bladder cystometrogram external urethral sphincter micturition urodynamics |
| title | Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| title_full | Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| title_fullStr | Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| title_full_unstemmed | Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| title_short | Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| title_sort | choice of cystometric technique impacts detrusor contractile dynamics in wistar rats |
| topic | bladder cystometrogram external urethral sphincter micturition urodynamics |
| url | https://doi.org/10.14814/phy2.14724 |
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