Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3
IntroductionTrace element metabolism disorders are often secondary to disorders of glucose metabolism in diabetes. Although 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] could ameliorate abnormal glucose metabolism in the development of diabetes, the effect on trace element metabolism is unclear. The obje...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1042558/full |
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| author | Dongxia Wang Dongxia Wang Ning Wang Juan Zhou Gang Luo Yanyan Li Wei Yu Hongxing Tan Gang Liu Jun Wang Liping Hao |
| author_facet | Dongxia Wang Dongxia Wang Ning Wang Juan Zhou Gang Luo Yanyan Li Wei Yu Hongxing Tan Gang Liu Jun Wang Liping Hao |
| author_sort | Dongxia Wang |
| collection | DOAJ |
| description | IntroductionTrace element metabolism disorders are often secondary to disorders of glucose metabolism in diabetes. Although 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] could ameliorate abnormal glucose metabolism in the development of diabetes, the effect on trace element metabolism is unclear. The objective of this study was to evaluate the influence of 1,25(OH)2D3 on urinary excretions of trace elements in Zucker diabetic fatty (ZDF) rats.MethodsAt 6 weeks of age, male ZDF (n = 40) rats were subdivided into four groups: diabetic model (ZDF), low-dose (ZDF + VL, 2 μg/kg⋅bw), middle-dose (ZDF + VM, 8 μg/kg⋅bw) and high-dose (ZDF + VH, 16 μg/kg⋅bw) 1,25(OH)2D3 groups. Another 10 Zucker lean (ZL) rats served as a control group. All rats were given vitamin D deficient Purina #5008 chow and the intervention groups were given the corresponding dose of 1,25(OH)2D3 by gavage on alternate days for 7 weeks. Microalbuminuria (MALB) and urinary creatinine concentration were detected by a biochemical autoanalyzer. Urine trace element concentrations were measured using inductively coupled plasma mass spectrometry (ICP-MS) and were corrected by urinary creatinine.ResultsThroughout the intervention phase, MALB, UACR and urinary creatinine levels in the ZDF group were significantly higher than those in the ZL group, and showed a gradual increase with the prolongation of the intervention time. These changes were reversed in a dose-dependent manner after 1,25(OH)2D3 intervention (P < 0.05). Correspondingly, most of the urinary trace element excretions in the ZDF rats were significantly increased compared with the ZL group, and 1,25(OH)2D3 intervention significantly reduced the urinary copper (Cu), zinc (Zn), selenium (Se) and molybdenum (Mo) levels in the ZDF rats (P < 0.05), especially in the medium and high dose groups.Conclusion1,25(OH)2D3 had improvement effects on urinary Cu, Zn, Se, and Mo excretions in ZDF rats, suggesting that it may be related to the reduction of diabetic renal impairment and renal oxidative damage. |
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| spelling | doaj.art-0eb4bd4fcd364f8a89f48625ef496e1a2022-12-22T04:40:01ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10425581042558Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3Dongxia Wang0Dongxia Wang1Ning Wang2Juan Zhou3Gang Luo4Yanyan Li5Wei Yu6Hongxing Tan7Gang Liu8Jun Wang9Liping Hao10Hubei Key Laboratory of Food Nutrition and Safety, Ministry of Education Key Laboratory of Environment, Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHebei Key Laboratory of Environment and Human Health, Department of Nutrition and Food Hygiene, School of Public Health, Hebei Medical University, Shijiazhuang, ChinaHubei Key Laboratory of Food Nutrition and Safety, Ministry of Education Key Laboratory of Environment, Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Food Nutrition and Safety, Ministry of Education Key Laboratory of Environment, Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Food Nutrition and Safety, Ministry of Education Key Laboratory of Environment, Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaShenzhen Center for Chronic Disease Control, Shenzhen, ChinaShenzhen Center for Chronic Disease Control, Shenzhen, ChinaShenzhen Center for Chronic Disease Control, Shenzhen, ChinaHebei Key Laboratory of Cardiac Injury Repair Mechanism Study, Department of Cardiology, Hebei International Joint Research Center for Structural Heart Disease, The First Hospital of Hebei Medical University, Shijiazhuang, ChinaSchool of Food and Drug, Shenzhen Polytechnic, Shenzhen, ChinaHubei Key Laboratory of Food Nutrition and Safety, Ministry of Education Key Laboratory of Environment, Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaIntroductionTrace element metabolism disorders are often secondary to disorders of glucose metabolism in diabetes. Although 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] could ameliorate abnormal glucose metabolism in the development of diabetes, the effect on trace element metabolism is unclear. The objective of this study was to evaluate the influence of 1,25(OH)2D3 on urinary excretions of trace elements in Zucker diabetic fatty (ZDF) rats.MethodsAt 6 weeks of age, male ZDF (n = 40) rats were subdivided into four groups: diabetic model (ZDF), low-dose (ZDF + VL, 2 μg/kg⋅bw), middle-dose (ZDF + VM, 8 μg/kg⋅bw) and high-dose (ZDF + VH, 16 μg/kg⋅bw) 1,25(OH)2D3 groups. Another 10 Zucker lean (ZL) rats served as a control group. All rats were given vitamin D deficient Purina #5008 chow and the intervention groups were given the corresponding dose of 1,25(OH)2D3 by gavage on alternate days for 7 weeks. Microalbuminuria (MALB) and urinary creatinine concentration were detected by a biochemical autoanalyzer. Urine trace element concentrations were measured using inductively coupled plasma mass spectrometry (ICP-MS) and were corrected by urinary creatinine.ResultsThroughout the intervention phase, MALB, UACR and urinary creatinine levels in the ZDF group were significantly higher than those in the ZL group, and showed a gradual increase with the prolongation of the intervention time. These changes were reversed in a dose-dependent manner after 1,25(OH)2D3 intervention (P < 0.05). Correspondingly, most of the urinary trace element excretions in the ZDF rats were significantly increased compared with the ZL group, and 1,25(OH)2D3 intervention significantly reduced the urinary copper (Cu), zinc (Zn), selenium (Se) and molybdenum (Mo) levels in the ZDF rats (P < 0.05), especially in the medium and high dose groups.Conclusion1,25(OH)2D3 had improvement effects on urinary Cu, Zn, Se, and Mo excretions in ZDF rats, suggesting that it may be related to the reduction of diabetic renal impairment and renal oxidative damage.https://www.frontiersin.org/articles/10.3389/fnut.2022.1042558/full1α25-dihydroxyvitamin D3diabetesZDF ratsrenal functionurinary Cu |
| spellingShingle | Dongxia Wang Dongxia Wang Ning Wang Juan Zhou Gang Luo Yanyan Li Wei Yu Hongxing Tan Gang Liu Jun Wang Liping Hao Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 Frontiers in Nutrition 1α 25-dihydroxyvitamin D3 diabetes ZDF rats renal function urinary Cu |
| title | Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 |
| title_full | Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 |
| title_fullStr | Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 |
| title_full_unstemmed | Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 |
| title_short | Urine trace element disorder along with renal function injury in vitamin D deficient diabetic rats and intervention effect of 1α,25-dihydroxyvitamin D3 |
| title_sort | urine trace element disorder along with renal function injury in vitamin d deficient diabetic rats and intervention effect of 1α 25 dihydroxyvitamin d3 |
| topic | 1α 25-dihydroxyvitamin D3 diabetes ZDF rats renal function urinary Cu |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1042558/full |
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