Development of a production chain from vegetable biowaste to platform chemicals
Abstract Background A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt to design a biotechnological cascade for the conversion of vegetable waste into acetoin and electrical energy. Results A vegetable waste dark ferment...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-018-0937-4 |
| _version_ | 1811264680484667392 |
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| author | Annemarie Schmidt Gunnar Sturm Christian Jonas Lapp Daniel Siebert Florencia Saravia Harald Horn Padma Priya Ravi Andreas Lemmer Johannes Gescher |
| author_facet | Annemarie Schmidt Gunnar Sturm Christian Jonas Lapp Daniel Siebert Florencia Saravia Harald Horn Padma Priya Ravi Andreas Lemmer Johannes Gescher |
| author_sort | Annemarie Schmidt |
| collection | DOAJ |
| description | Abstract Background A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt to design a biotechnological cascade for the conversion of vegetable waste into acetoin and electrical energy. Results A vegetable waste dark fermentation effluent containing mainly acetate, butyrate and propionate was oxidized in a bioelectrochemical system. The achieved average current at a constant anode potential of 0 mV against standard hydrogen electrode was 177.5 ± 52.5 µA/cm2. During this step, acetate and butyrate were removed from the effluent while propionate was the major remaining component of the total organic carbon content comprising on average 75.6%. The key players with regard to carbon oxidation and electrode reduction were revealed using amplicon sequencing and metatranscriptomic analysis. Using nanofiltration, it was possible to concentrate the propionate in the effluent. The effluent was revealed to be a suitable medium for biotechnological production strains. As a proof of principle, the propionate in the effluent of the bioelectrochemical system was converted into the platform chemical acetoin with a carbon recovery of 86%. Conclusions To the best of our knowledge this is the first report on a full biotechnological production chain leading from vegetable waste to the production of a single valuable platform chemical that integrates carbon elimination steps leading to the production of the valuable side product electrical energy. |
| first_indexed | 2024-04-12T20:08:44Z |
| format | Article |
| id | doaj.art-0eb592aa1f784143acb7362c7c0c0405 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-12T20:08:44Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-0eb592aa1f784143acb7362c7c0c04052022-12-22T03:18:19ZengBMCMicrobial Cell Factories1475-28592018-06-0117111210.1186/s12934-018-0937-4Development of a production chain from vegetable biowaste to platform chemicalsAnnemarie Schmidt0Gunnar Sturm1Christian Jonas Lapp2Daniel Siebert3Florencia Saravia4Harald Horn5Padma Priya Ravi6Andreas Lemmer7Johannes Gescher8Department Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of TechnologyDepartment Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of TechnologyDepartment Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of TechnologyInstitute of Microbiology and Biotechnology, University of UlmChair of Water Chemistry and Water Technology, Karlsruhe Institute of Technology, Engler-Bunte-InstitutChair of Water Chemistry and Water Technology, Karlsruhe Institute of Technology, Engler-Bunte-InstitutState Institute of Agricultural Engineering and Bioenergy, University of HohenheimState Institute of Agricultural Engineering and Bioenergy, University of HohenheimDepartment Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of TechnologyAbstract Background A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt to design a biotechnological cascade for the conversion of vegetable waste into acetoin and electrical energy. Results A vegetable waste dark fermentation effluent containing mainly acetate, butyrate and propionate was oxidized in a bioelectrochemical system. The achieved average current at a constant anode potential of 0 mV against standard hydrogen electrode was 177.5 ± 52.5 µA/cm2. During this step, acetate and butyrate were removed from the effluent while propionate was the major remaining component of the total organic carbon content comprising on average 75.6%. The key players with regard to carbon oxidation and electrode reduction were revealed using amplicon sequencing and metatranscriptomic analysis. Using nanofiltration, it was possible to concentrate the propionate in the effluent. The effluent was revealed to be a suitable medium for biotechnological production strains. As a proof of principle, the propionate in the effluent of the bioelectrochemical system was converted into the platform chemical acetoin with a carbon recovery of 86%. Conclusions To the best of our knowledge this is the first report on a full biotechnological production chain leading from vegetable waste to the production of a single valuable platform chemical that integrates carbon elimination steps leading to the production of the valuable side product electrical energy.http://link.springer.com/article/10.1186/s12934-018-0937-4BiowasteVegetable wasteBioelectrochemical systemAcetoinPropionateOrganic acids |
| spellingShingle | Annemarie Schmidt Gunnar Sturm Christian Jonas Lapp Daniel Siebert Florencia Saravia Harald Horn Padma Priya Ravi Andreas Lemmer Johannes Gescher Development of a production chain from vegetable biowaste to platform chemicals Microbial Cell Factories Biowaste Vegetable waste Bioelectrochemical system Acetoin Propionate Organic acids |
| title | Development of a production chain from vegetable biowaste to platform chemicals |
| title_full | Development of a production chain from vegetable biowaste to platform chemicals |
| title_fullStr | Development of a production chain from vegetable biowaste to platform chemicals |
| title_full_unstemmed | Development of a production chain from vegetable biowaste to platform chemicals |
| title_short | Development of a production chain from vegetable biowaste to platform chemicals |
| title_sort | development of a production chain from vegetable biowaste to platform chemicals |
| topic | Biowaste Vegetable waste Bioelectrochemical system Acetoin Propionate Organic acids |
| url | http://link.springer.com/article/10.1186/s12934-018-0937-4 |
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