In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population...
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MDPI AG
2023-07-01
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| author | Bolat Seisenov Dulat Duimbayev Nurlybay Kazhgaliyev Talgat Abdrakhmanov Alexandra Tegza Rustem Abeldinov Nadezhda Burambayeva Alma Temirzhanova Ivan Tegza Zhomart Kemeshev Assylbek Zhanabayev Nurbolat Akhmetbekov Marat Aisin Kuandyk Zhugunissov Arman Issimov |
| author_facet | Bolat Seisenov Dulat Duimbayev Nurlybay Kazhgaliyev Talgat Abdrakhmanov Alexandra Tegza Rustem Abeldinov Nadezhda Burambayeva Alma Temirzhanova Ivan Tegza Zhomart Kemeshev Assylbek Zhanabayev Nurbolat Akhmetbekov Marat Aisin Kuandyk Zhugunissov Arman Issimov |
| author_sort | Bolat Seisenov |
| collection | DOAJ |
| description | In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (<i>p</i> ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms. |
| first_indexed | 2024-03-10T23:47:09Z |
| format | Article |
| id | doaj.art-0eb76ba3bf3a4d1f8c8773d4f3dbd6b2 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T23:47:09Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-0eb76ba3bf3a4d1f8c8773d4f3dbd6b22023-11-19T01:53:19ZengMDPI AGLife2075-17292023-07-01138163210.3390/life13081632In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative StudyBolat Seisenov0Dulat Duimbayev1Nurlybay Kazhgaliyev2Talgat Abdrakhmanov3Alexandra Tegza4Rustem Abeldinov5Nadezhda Burambayeva6Alma Temirzhanova7Ivan Tegza8Zhomart Kemeshev9Assylbek Zhanabayev10Nurbolat Akhmetbekov11Marat Aisin12Kuandyk Zhugunissov13Arman Issimov14Assyl Tulik, Republican Center for Livestock Breeding, Astana 010000, KazakhstanDepartment of Animal Husbandary and Bioresourses, Zhangir Khan West Kazakhstan Agrarian—Technical University, Oral 090000, KazakhstanFaculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, KazakhstanFaculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, KazakhstanDepartment of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, KazakhstanDepartment of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, KazakhstanDepartment of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, KazakhstanDepartment of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, KazakhstanDepartment of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, KazakhstanFaculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, KazakhstanFaculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, KazakhstanFaculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, KazakhstanDepartment of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanDepartment of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, KazakhstanIn vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (<i>p</i> ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.https://www.mdpi.com/2075-1729/13/8/1632IVFembryoKazakh Whiteheaded cattle |
| spellingShingle | Bolat Seisenov Dulat Duimbayev Nurlybay Kazhgaliyev Talgat Abdrakhmanov Alexandra Tegza Rustem Abeldinov Nadezhda Burambayeva Alma Temirzhanova Ivan Tegza Zhomart Kemeshev Assylbek Zhanabayev Nurbolat Akhmetbekov Marat Aisin Kuandyk Zhugunissov Arman Issimov In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study Life IVF embryo Kazakh Whiteheaded cattle |
| title | In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study |
| title_full | In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study |
| title_fullStr | In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study |
| title_full_unstemmed | In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study |
| title_short | In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study |
| title_sort | in vitro fertilization in kazakh whiteheaded cattle a comparative study |
| topic | IVF embryo Kazakh Whiteheaded cattle |
| url | https://www.mdpi.com/2075-1729/13/8/1632 |
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