Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.846092/full |
| _version_ | 1817977352420851712 |
|---|---|
| author | Joan Domingo-Reinés Gonzalo Martínez-Navajas Gonzalo Martínez-Navajas Rosa Montes Rosa Montes Mar Lamolda Iris Simón Iris Simón Julio Castaño Rosa Ríos-Pelegrina Rosa Ríos-Pelegrina Javier Luis Lopez-Hidalgo Javier Luis Lopez-Hidalgo Raimundo García del Moral Raimundo García del Moral Juan A. Marchal Juan A. Marchal Juan A. Marchal Juan A. Marchal Pedro J. Real Pedro J. Real Pedro J. Real Verónica Ramos-Mejía |
| author_facet | Joan Domingo-Reinés Gonzalo Martínez-Navajas Gonzalo Martínez-Navajas Rosa Montes Rosa Montes Mar Lamolda Iris Simón Iris Simón Julio Castaño Rosa Ríos-Pelegrina Rosa Ríos-Pelegrina Javier Luis Lopez-Hidalgo Javier Luis Lopez-Hidalgo Raimundo García del Moral Raimundo García del Moral Juan A. Marchal Juan A. Marchal Juan A. Marchal Juan A. Marchal Pedro J. Real Pedro J. Real Pedro J. Real Verónica Ramos-Mejía |
| author_sort | Joan Domingo-Reinés |
| collection | DOAJ |
| description | Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process. |
| first_indexed | 2024-04-13T22:15:15Z |
| format | Article |
| id | doaj.art-0eb7a0b1ec7949e59ed05681b69ce025 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T22:15:15Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-0eb7a0b1ec7949e59ed05681b69ce0252022-12-22T02:27:34ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-06-011010.3389/fcell.2022.846092846092Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor LocusJoan Domingo-Reinés0Gonzalo Martínez-Navajas1Gonzalo Martínez-Navajas2Rosa Montes3Rosa Montes4Mar Lamolda5Iris Simón6Iris Simón7Julio Castaño8Rosa Ríos-Pelegrina9Rosa Ríos-Pelegrina10Javier Luis Lopez-Hidalgo11Javier Luis Lopez-Hidalgo12Raimundo García del Moral13Raimundo García del Moral14Juan A. Marchal15Juan A. Marchal16Juan A. Marchal17Juan A. Marchal18Pedro J. Real19Pedro J. Real20Pedro J. Real21Verónica Ramos-Mejía22GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainFaculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainFaculty of Sciences, Department of Cell Biology, University of Granada, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainFaculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, SpainAdvanced Cell Therapy Service, Banc de Sang I Teixits, Edifici Dr. Frederic Duran I Jordà, Barcelona, SpainUnidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, SpainInstituto de Investigación Biosanitaria Ibs.GRANADA, Granada, SpainUnidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, SpainInstituto de Investigación Biosanitaria Ibs.GRANADA, Granada, SpainUnidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, SpainInstituto de Investigación Biosanitaria Ibs.GRANADA, Granada, SpainInstituto de Investigación Biosanitaria Ibs.GRANADA, Granada, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, SpainFaculty of Medicine, Department of Human Anatomy and Embryology, University of Granada, Granada, SpainExcellence Research Unit “Modeling Nature” (MNat), University of Granada, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainFaculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, SpainInstituto de Investigación Biosanitaria Ibs.GRANADA, Granada, SpainGENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, SpainPediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process.https://www.frontiersin.org/articles/10.3389/fcell.2022.846092/fullacute myeliod leukemiapediatricdisease modelhuman stem cellhematopoieisis |
| spellingShingle | Joan Domingo-Reinés Gonzalo Martínez-Navajas Gonzalo Martínez-Navajas Rosa Montes Rosa Montes Mar Lamolda Iris Simón Iris Simón Julio Castaño Rosa Ríos-Pelegrina Rosa Ríos-Pelegrina Javier Luis Lopez-Hidalgo Javier Luis Lopez-Hidalgo Raimundo García del Moral Raimundo García del Moral Juan A. Marchal Juan A. Marchal Juan A. Marchal Juan A. Marchal Pedro J. Real Pedro J. Real Pedro J. Real Verónica Ramos-Mejía Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus Frontiers in Cell and Developmental Biology acute myeliod leukemia pediatric disease model human stem cell hematopoieisis |
| title | Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus |
| title_full | Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus |
| title_fullStr | Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus |
| title_full_unstemmed | Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus |
| title_short | Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus |
| title_sort | generation of a h9 clonal cell line with inducible expression of nup98 kdm5a fusion gene in the aavs1 safe harbor locus |
| topic | acute myeliod leukemia pediatric disease model human stem cell hematopoieisis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.846092/full |
| work_keys_str_mv | AT joandomingoreines generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT gonzalomartineznavajas generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT gonzalomartineznavajas generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT rosamontes generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT rosamontes generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT marlamolda generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT irissimon generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT irissimon generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT juliocastano generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT rosariospelegrina generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT rosariospelegrina generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT javierluislopezhidalgo generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT javierluislopezhidalgo generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT raimundogarciadelmoral generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT raimundogarciadelmoral generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT juanamarchal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT juanamarchal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT juanamarchal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT juanamarchal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT pedrojreal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT pedrojreal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT pedrojreal generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus AT veronicaramosmejia generationofah9clonalcelllinewithinducibleexpressionofnup98kdm5afusiongeneintheaavs1safeharborlocus |