GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION
Aim. The aim was to study the dynamics of the growth and carotene synthesis by the strains B. amyloliquefaciens UCM B-5113 and Bacillus 1.1 at submerged cultivation. Methods. Bacteria were cultured on synthetic medium and medium with molasses in periodic conditions. The amount of absolutely dry bact...
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2015-06-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
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| Online Access: | http://mbt.onu.edu.ua/article/view/48073 |
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| author | О. О. Нечипуренко М. А. Хархота К. С. Бордунос Л. В. Авдєєва |
| author_facet | О. О. Нечипуренко М. А. Хархота К. С. Бордунос Л. В. Авдєєва |
| author_sort | О. О. Нечипуренко |
| collection | DOAJ |
| description | Aim. The aim was to study the dynamics of the growth and carotene synthesis by the strains B. amyloliquefaciens UCM B-5113 and Bacillus 1.1 at submerged cultivation. Methods. Bacteria were cultured on synthetic medium and medium with molasses in periodic conditions. The amount of absolutely dry bacterial mass (g/l), the total content of carotenoids (mg/l) were determined spectrophotometrically at a wavelength of 540 and 440 nm, respectively. The pigments extraction was carried out with the use of mixture of chloroform and methanol (2:1). Results. The maximum bacterial cells concentration of the strains B. subtilis 1.1 B. amyloliquefaciens UCM B-5113 after cultivation on medium with molasses were 3.6 ± 0.2 ×1010 and 4.5 ± 0.3 × 1010 CFU/ml, respectively, and 10 times higher than the values obtained in the synthetic medium. Biomass yield of the strain B. subtilis 1.1 on complex medium was 4.5 g/l, and on synthetic –1.7 g/l; the data for B. amyloliquefaciens UCM B-5113 – 5.0 g/l and 2 g/l, respectively. Total carotenoid content of the strain B. subtilis 1.1 on complex medium was 965 mg/l of completely dry biomass (CDB), on the synthetic – 197 mg/l; the data for the strain B. amyloliquefaciens UCM B-5113 – 1356 mg/l and 173 mg/l, respectively. Conclusion. The complex medium with molasses needs to be used to obtain a high amount of bacterial biomass and carotenoid pigments of investigated strains. The quantity of viable cells of the strains B. subtilis 1.1 and B. amyloliquefaciens UCM B-5113 after cultivation on medium with molasses was 10 times higher than on synthetic, the maximum biomass – 3 and 2.5 times higher, the total carotenoid content – 5 and 8 times higher, respectively. |
| first_indexed | 2024-12-19T16:07:54Z |
| format | Article |
| id | doaj.art-0eb8f3534e744b559a9819ced96427c4 |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T16:07:54Z |
| publishDate | 2015-06-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-0eb8f3534e744b559a9819ced96427c42022-12-21T20:14:48ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632015-06-0102(30)414810.18524/2307-4663.2015.2(30).4807348073GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATIONО. О. Нечипуренко0М. А. Хархота1К. С. Бордунос2Л. В. Авдєєва3Інститут мікробіології і вірусології імені Д.К. Заболотного НАН УкраїниІнститут мікробіології і вірусології імені Д.К. Заболотного НАН УкраїниДніпропетровський національний університет імені Олеся ГончараІнститут мікробіології і вірусології імені Д.К. Заболотного НАН УкраїниAim. The aim was to study the dynamics of the growth and carotene synthesis by the strains B. amyloliquefaciens UCM B-5113 and Bacillus 1.1 at submerged cultivation. Methods. Bacteria were cultured on synthetic medium and medium with molasses in periodic conditions. The amount of absolutely dry bacterial mass (g/l), the total content of carotenoids (mg/l) were determined spectrophotometrically at a wavelength of 540 and 440 nm, respectively. The pigments extraction was carried out with the use of mixture of chloroform and methanol (2:1). Results. The maximum bacterial cells concentration of the strains B. subtilis 1.1 B. amyloliquefaciens UCM B-5113 after cultivation on medium with molasses were 3.6 ± 0.2 ×1010 and 4.5 ± 0.3 × 1010 CFU/ml, respectively, and 10 times higher than the values obtained in the synthetic medium. Biomass yield of the strain B. subtilis 1.1 on complex medium was 4.5 g/l, and on synthetic –1.7 g/l; the data for B. amyloliquefaciens UCM B-5113 – 5.0 g/l and 2 g/l, respectively. Total carotenoid content of the strain B. subtilis 1.1 on complex medium was 965 mg/l of completely dry biomass (CDB), on the synthetic – 197 mg/l; the data for the strain B. amyloliquefaciens UCM B-5113 – 1356 mg/l and 173 mg/l, respectively. Conclusion. The complex medium with molasses needs to be used to obtain a high amount of bacterial biomass and carotenoid pigments of investigated strains. The quantity of viable cells of the strains B. subtilis 1.1 and B. amyloliquefaciens UCM B-5113 after cultivation on medium with molasses was 10 times higher than on synthetic, the maximum biomass – 3 and 2.5 times higher, the total carotenoid content – 5 and 8 times higher, respectively.http://mbt.onu.edu.ua/article/view/48073каротинb. amyloliquefaciensb. subtilisпігментоутворення |
| spellingShingle | О. О. Нечипуренко М. А. Хархота К. С. Бордунос Л. В. Авдєєва GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION Mìkrobìologìâ ì Bìotehnologìâ каротин b. amyloliquefaciens b. subtilis пігментоутворення |
| title | GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION |
| title_full | GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION |
| title_fullStr | GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION |
| title_full_unstemmed | GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION |
| title_short | GROWTH AND CAROTENE SYNTHESIS BY THE STRAINS BACILLUS AMYLOLIQUEFACIENS UCM B-5113 AND B. SUBTILIS 1.1 AT SUBMERGED CULTIVATION |
| title_sort | growth and carotene synthesis by the strains bacillus amyloliquefaciens ucm b 5113 and b subtilis 1 1 at submerged cultivation |
| topic | каротин b. amyloliquefaciens b. subtilis пігментоутворення |
| url | http://mbt.onu.edu.ua/article/view/48073 |
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