Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota
To date, studies on apoptosis in echinoderms mainly focus on the caspase-dependent extrinsic apoptotic pathway, while knowledges regarding the intrinsic apoptotic pathway is still limited. In this study, 55 genes belong to 7 families of the intrinsic apoptotic pathway, namely, Bcl-2, caspase, AIF, C...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002041 |
| _version_ | 1797752957592141824 |
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| author | Lian Liu Xiaomin Li Xiaofen Wu Juan Feng Xiangxing Zhu Dongsheng Tang Xiao Jiang Wenjie Pan Jiasheng Huang Ting Chen Chunhua Ren Aifen Yan |
| author_facet | Lian Liu Xiaomin Li Xiaofen Wu Juan Feng Xiangxing Zhu Dongsheng Tang Xiao Jiang Wenjie Pan Jiasheng Huang Ting Chen Chunhua Ren Aifen Yan |
| author_sort | Lian Liu |
| collection | DOAJ |
| description | To date, studies on apoptosis in echinoderms mainly focus on the caspase-dependent extrinsic apoptotic pathway, while knowledges regarding the intrinsic apoptotic pathway is still limited. In this study, 55 genes belong to 7 families of the intrinsic apoptotic pathway, namely, Bcl-2, caspase, AIF, Cyt c, Apaf-1, Smac, HTRA2 and Endo G, were identified by screening of the genomic database of the tropical sea cucumber Holothuria leucospilota. The Bcl-2 family showed significant gene expansion in H. leucospilota but not in other echinoderms, while the pro-apoptotic factor Endo G is generally lost in echinoderms. Transcriptomic data showed that the genes of the intrinsic apoptotic pathway were ubiquitously expressed in all tissues, and all embryonic and larval developmental stages of H. leucospilota. The gene numbers and functional domains of the caspase family and AIF family were further clarified and showed conserved with their counterparts in vertebrates. In primary coelomocytes, the transcript expression of different caspases showed different responses to pathogenic challenges of vibrio and pathogen-associated molecular patterns, and environmental challenges of heave metal and reactive oxygen species. Overall, this study indicates that the intrinsic apoptotic pathway is conserved between vertebrates and echinoderms by using the sea cucumber H. leucospilota as a model. This study may contribute in increasing the knowledge of development and innate immunity in ancestral deuterostomia species. |
| first_indexed | 2024-03-12T17:10:56Z |
| format | Article |
| id | doaj.art-0ebb0861e3bc4e7b80f937d5ecc64d17 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-12T17:10:56Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-0ebb0861e3bc4e7b80f937d5ecc64d172023-08-06T04:37:41ZengElsevierAquaculture Reports2352-51342023-08-0131101665Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilotaLian Liu0Xiaomin Li1Xiaofen Wu2Juan Feng3Xiangxing Zhu4Dongsheng Tang5Xiao Jiang6Wenjie Pan7Jiasheng Huang8Ting Chen9Chunhua Ren10Aifen Yan11School of Medicine, Foshan University, Foshan 528000, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaInstitute for Integrative Biology of the Cell, University of Paris-Saclay, 91198 Paris, FranceSchool of Medicine, Foshan University, Foshan 528000, ChinaSchool of Medicine, Foshan University, Foshan 528000, ChinaSchool of Medicine, Foshan University, Foshan 528000, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Corresponding authors.School of Medicine, Foshan University, Foshan 528000, China; Corresponding authors.To date, studies on apoptosis in echinoderms mainly focus on the caspase-dependent extrinsic apoptotic pathway, while knowledges regarding the intrinsic apoptotic pathway is still limited. In this study, 55 genes belong to 7 families of the intrinsic apoptotic pathway, namely, Bcl-2, caspase, AIF, Cyt c, Apaf-1, Smac, HTRA2 and Endo G, were identified by screening of the genomic database of the tropical sea cucumber Holothuria leucospilota. The Bcl-2 family showed significant gene expansion in H. leucospilota but not in other echinoderms, while the pro-apoptotic factor Endo G is generally lost in echinoderms. Transcriptomic data showed that the genes of the intrinsic apoptotic pathway were ubiquitously expressed in all tissues, and all embryonic and larval developmental stages of H. leucospilota. The gene numbers and functional domains of the caspase family and AIF family were further clarified and showed conserved with their counterparts in vertebrates. In primary coelomocytes, the transcript expression of different caspases showed different responses to pathogenic challenges of vibrio and pathogen-associated molecular patterns, and environmental challenges of heave metal and reactive oxygen species. Overall, this study indicates that the intrinsic apoptotic pathway is conserved between vertebrates and echinoderms by using the sea cucumber H. leucospilota as a model. This study may contribute in increasing the knowledge of development and innate immunity in ancestral deuterostomia species.http://www.sciencedirect.com/science/article/pii/S2352513423002041ApoptosisThe Intrinsic PathwayCaspase, Pathogenic and environmental challengeEchinoderm |
| spellingShingle | Lian Liu Xiaomin Li Xiaofen Wu Juan Feng Xiangxing Zhu Dongsheng Tang Xiao Jiang Wenjie Pan Jiasheng Huang Ting Chen Chunhua Ren Aifen Yan Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota Aquaculture Reports Apoptosis The Intrinsic Pathway Caspase, Pathogenic and environmental challenge Echinoderm |
| title | Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota |
| title_full | Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota |
| title_fullStr | Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota |
| title_full_unstemmed | Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota |
| title_short | Genome-wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota |
| title_sort | genome wide analysis of the intrinsic apoptotic pathway in the tropical sea cucumber holothuria leucospilota |
| topic | Apoptosis The Intrinsic Pathway Caspase, Pathogenic and environmental challenge Echinoderm |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002041 |
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