Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer
Abstract Exosomes in the body fluid are effective cell-derived membranous structures transferring various molecules to mediate intercellular communication. The expression of protein in the urinary exosomes from the colorectal cancer (CRC) patients could reflect the characteristics of tumorigenesis....
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-02-01
|
| Series: | Proteome Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12953-023-00203-y |
| _version_ | 1811166040515674112 |
|---|---|
| author | Ling Ma Haijiao Yu Yubing Zhu Kaiyu Xu Aimin Zhao Lei Ding Hong Gao Man Zhang |
| author_facet | Ling Ma Haijiao Yu Yubing Zhu Kaiyu Xu Aimin Zhao Lei Ding Hong Gao Man Zhang |
| author_sort | Ling Ma |
| collection | DOAJ |
| description | Abstract Exosomes in the body fluid are effective cell-derived membranous structures transferring various molecules to mediate intercellular communication. The expression of protein in the urinary exosomes from the colorectal cancer (CRC) patients could reflect the characteristics of tumorigenesis. The urinary exosomes with globular membrane structure, the size of 30 ~ 100 nm and positive expression of CD9, CD63 and CD81 were successfully isolated from 9 CRC patients and 3 heathy adults using the density gradient ultracentrifugation. Proteome profiles revealed by label-free liquid chromatography-tandem mass spectrometry (LC–MS/MS) indicated that several proteins were differentially expressed among different stages of CRC. Compared with normal controls, 67 proteins in CRC urinary exosomes were upregulated and 74 proteins were downregulated. The bioinformatics analysis revealed the decreased proteins were related to ESCRT III complex disassembly. The CHMP family was further determined to be the hub of interaction network of proteins enriched in ESCRT signaling. The significant decrease of CHMP4A, CHMP4B, CHMP2A, CHMP2B and CHMP1B were respectively found in the total CRC group and distant metastasis group compared with NC group. Moreover, the CEACAM family also showed significant aberrant changes in the urinary exosomes of CRC patients. The CEACAM7 and CEACAM1 were increased in the CRC patients compared with healthy individuals (P < 0.05). Significant changes of proteomic profile could be found in the urinary exosomes in the CRC patients. The differential expressed urinary exosomes derived proteins showed potential usage in diagnosis and prognosis of CRC. |
| first_indexed | 2024-04-10T15:45:59Z |
| format | Article |
| id | doaj.art-0ebb1fc193ca4a7bb70d8644cc480484 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-04-10T15:45:59Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-0ebb1fc193ca4a7bb70d8644cc4804842023-02-12T12:05:00ZengBMCProteome Science1477-59562023-02-012111910.1186/s12953-023-00203-yIsolation and proteomic profiling of urinary exosomes from patients with colorectal cancerLing Ma0Haijiao Yu1Yubing Zhu2Kaiyu Xu3Aimin Zhao4Lei Ding5Hong Gao6Man Zhang7Department of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Gastrointestinal Tumor Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityDepartment of Clinical Laboratory, Beijing Shijitan Hospital Affiliated to Capital Medical UniversityAbstract Exosomes in the body fluid are effective cell-derived membranous structures transferring various molecules to mediate intercellular communication. The expression of protein in the urinary exosomes from the colorectal cancer (CRC) patients could reflect the characteristics of tumorigenesis. The urinary exosomes with globular membrane structure, the size of 30 ~ 100 nm and positive expression of CD9, CD63 and CD81 were successfully isolated from 9 CRC patients and 3 heathy adults using the density gradient ultracentrifugation. Proteome profiles revealed by label-free liquid chromatography-tandem mass spectrometry (LC–MS/MS) indicated that several proteins were differentially expressed among different stages of CRC. Compared with normal controls, 67 proteins in CRC urinary exosomes were upregulated and 74 proteins were downregulated. The bioinformatics analysis revealed the decreased proteins were related to ESCRT III complex disassembly. The CHMP family was further determined to be the hub of interaction network of proteins enriched in ESCRT signaling. The significant decrease of CHMP4A, CHMP4B, CHMP2A, CHMP2B and CHMP1B were respectively found in the total CRC group and distant metastasis group compared with NC group. Moreover, the CEACAM family also showed significant aberrant changes in the urinary exosomes of CRC patients. The CEACAM7 and CEACAM1 were increased in the CRC patients compared with healthy individuals (P < 0.05). Significant changes of proteomic profile could be found in the urinary exosomes in the CRC patients. The differential expressed urinary exosomes derived proteins showed potential usage in diagnosis and prognosis of CRC.https://doi.org/10.1186/s12953-023-00203-yColorectal cancerUrineExosomeMass spectrometryBioinformatics analysis |
| spellingShingle | Ling Ma Haijiao Yu Yubing Zhu Kaiyu Xu Aimin Zhao Lei Ding Hong Gao Man Zhang Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer Proteome Science Colorectal cancer Urine Exosome Mass spectrometry Bioinformatics analysis |
| title | Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| title_full | Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| title_fullStr | Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| title_full_unstemmed | Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| title_short | Isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| title_sort | isolation and proteomic profiling of urinary exosomes from patients with colorectal cancer |
| topic | Colorectal cancer Urine Exosome Mass spectrometry Bioinformatics analysis |
| url | https://doi.org/10.1186/s12953-023-00203-y |
| work_keys_str_mv | AT lingma isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT haijiaoyu isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT yubingzhu isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT kaiyuxu isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT aiminzhao isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT leiding isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT honggao isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer AT manzhang isolationandproteomicprofilingofurinaryexosomesfrompatientswithcolorectalcancer |