LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway
Xu Cao, Qiu-Ling Fan Department of Nephrology, The First Hospital of China Medical University, Shenyang, People’s Republic of ChinaCorrespondence: Qiu-Ling FanDepartment of Nephrology, The First Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang 110...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-11-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/lncrna-mir503hg-promotes-high-glucose-induced-proximal-tubular-cell-ap-peer-reviewed-article-DMSO |
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| author | Cao X Fan QL |
| author_facet | Cao X Fan QL |
| author_sort | Cao X |
| collection | DOAJ |
| description | Xu Cao, Qiu-Ling Fan Department of Nephrology, The First Hospital of China Medical University, Shenyang, People’s Republic of ChinaCorrespondence: Qiu-Ling FanDepartment of Nephrology, The First Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang 110001, People’s Republic of ChinaTel +86 13904012680Email cmufql@163.comAim: More than half of microRNAs are located in genes. LncRNAs are host genes of intronic microRNAs that regulate intracellular splicing to form pre-miRNAs that are processed to mature miRNAs. MicroRNAs work as partners or antagonists of their host lncRNAs by fine-tuning their target genes. However, whether lncRNA-MIR503HG (miR-503 host gene) is co-transcribed with miR-503 and affects miR-503 splicing, thereby affecting its target gene Bcl-2 expression and cell mitochondrial apoptotic pathway in diabetic nephropathy (DN) is currently unknown.Methods: Human proximal tubular (HK-2) cells cultured in high glucose were transfected with lncRNA MIR503HG overexpression/inhibition plasmid and miR-503 mimics/inhibitor. Real-time quantitative PCR was used to measure the expression levels of lncRNA MIR503HG, pre-miR-503, miR-503 and Bcl-2. Western blot was used to measure the protein expressions of Bcl-2, Bax, Cytc and cleaved-caspase 9/3. Annexin V/PI flow cytometry was used to measure apoptosis.Results: Host lncRNA MIR503HG was co-transcribed with miR-503. MIR503HG regulated the expression of miR-503 by affecting miR-503 splicing synthesis. In the presence of high glucose, the expression levels of lncRNA MIR503HG and miR-503 were up-regulated in HK-2 cells cultured in high glucose. Bcl-2 expression was inhibited and levels of apoptosis-related proteins Cytc and Bax were increased in HK-2 cells cultured in high glucose, all of which promoted the caspase cascade reaction, leading to increased caspase-9 and caspase-3 shear fragments inducing apoptosis of the mitochondrial pathway. Inhibition of MIR503HG led to a reduction in miR-503 expression, up-regulated its target gene Bcl-2, inhibited the expression levels of Bax and other apoptosis-related proteins and attenuated HK-2 cell apoptosis induced by high glucose. Co-transfection of miRNA-503 partially offset the effect of MIR503HG-siRNA.Conclusion: MIR503HG indirectly regulates Bcl-2 by promoting the co-transcription of miRNA-503 to participate high-glucose-induced proximal tubular cell apoptosis, providing a new target for diabetic nephropathy treatment.Keywords: diabetic nephropathy, lncRNA MIR503HG, miR-503, host gene, apoptosis |
| first_indexed | 2024-04-10T18:25:11Z |
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| id | doaj.art-0ebb9fb87cd541f1b709407e8dc600d4 |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:25:11Z |
| publishDate | 2020-11-01 |
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| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-0ebb9fb87cd541f1b709407e8dc600d42023-02-02T05:47:46ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072020-11-01Volume 134507451759559LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 PathwayCao XFan QLXu Cao, Qiu-Ling Fan Department of Nephrology, The First Hospital of China Medical University, Shenyang, People’s Republic of ChinaCorrespondence: Qiu-Ling FanDepartment of Nephrology, The First Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang 110001, People’s Republic of ChinaTel +86 13904012680Email cmufql@163.comAim: More than half of microRNAs are located in genes. LncRNAs are host genes of intronic microRNAs that regulate intracellular splicing to form pre-miRNAs that are processed to mature miRNAs. MicroRNAs work as partners or antagonists of their host lncRNAs by fine-tuning their target genes. However, whether lncRNA-MIR503HG (miR-503 host gene) is co-transcribed with miR-503 and affects miR-503 splicing, thereby affecting its target gene Bcl-2 expression and cell mitochondrial apoptotic pathway in diabetic nephropathy (DN) is currently unknown.Methods: Human proximal tubular (HK-2) cells cultured in high glucose were transfected with lncRNA MIR503HG overexpression/inhibition plasmid and miR-503 mimics/inhibitor. Real-time quantitative PCR was used to measure the expression levels of lncRNA MIR503HG, pre-miR-503, miR-503 and Bcl-2. Western blot was used to measure the protein expressions of Bcl-2, Bax, Cytc and cleaved-caspase 9/3. Annexin V/PI flow cytometry was used to measure apoptosis.Results: Host lncRNA MIR503HG was co-transcribed with miR-503. MIR503HG regulated the expression of miR-503 by affecting miR-503 splicing synthesis. In the presence of high glucose, the expression levels of lncRNA MIR503HG and miR-503 were up-regulated in HK-2 cells cultured in high glucose. Bcl-2 expression was inhibited and levels of apoptosis-related proteins Cytc and Bax were increased in HK-2 cells cultured in high glucose, all of which promoted the caspase cascade reaction, leading to increased caspase-9 and caspase-3 shear fragments inducing apoptosis of the mitochondrial pathway. Inhibition of MIR503HG led to a reduction in miR-503 expression, up-regulated its target gene Bcl-2, inhibited the expression levels of Bax and other apoptosis-related proteins and attenuated HK-2 cell apoptosis induced by high glucose. Co-transfection of miRNA-503 partially offset the effect of MIR503HG-siRNA.Conclusion: MIR503HG indirectly regulates Bcl-2 by promoting the co-transcription of miRNA-503 to participate high-glucose-induced proximal tubular cell apoptosis, providing a new target for diabetic nephropathy treatment.Keywords: diabetic nephropathy, lncRNA MIR503HG, miR-503, host gene, apoptosishttps://www.dovepress.com/lncrna-mir503hg-promotes-high-glucose-induced-proximal-tubular-cell-ap-peer-reviewed-article-DMSOdiabetic nephropathylncrna mir503hgmir-503host geneapoptosis |
| spellingShingle | Cao X Fan QL LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway Diabetes, Metabolic Syndrome and Obesity diabetic nephropathy lncrna mir503hg mir-503 host gene apoptosis |
| title | LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway |
| title_full | LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway |
| title_fullStr | LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway |
| title_full_unstemmed | LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway |
| title_short | LncRNA MIR503HG Promotes High-Glucose-Induced Proximal Tubular Cell Apoptosis by Targeting miR-503-5p/Bcl-2 Pathway |
| title_sort | lncrna mir503hg promotes high glucose induced proximal tubular cell apoptosis by targeting mir 503 5p bcl 2 pathway |
| topic | diabetic nephropathy lncrna mir503hg mir-503 host gene apoptosis |
| url | https://www.dovepress.com/lncrna-mir503hg-promotes-high-glucose-induced-proximal-tubular-cell-ap-peer-reviewed-article-DMSO |
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