Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin
Raman spectroscopy was used to detect low quantities of Vismodegib in the skin after its topical application via transfersomes. Vismodegib is a novel antineoplastic drug approved for oral administration for treatment of basal cell carcinoma. Transfersomes loaded with Vismodegib were prepared by thin...
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MDPI AG
2023-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/13/7687 |
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| author | Gisela Eliane Gómez María Natalia Calienni Silvia del Valle Alonso Fernando Carlos Alvira Jorge Montanari |
| author_facet | Gisela Eliane Gómez María Natalia Calienni Silvia del Valle Alonso Fernando Carlos Alvira Jorge Montanari |
| author_sort | Gisela Eliane Gómez |
| collection | DOAJ |
| description | Raman spectroscopy was used to detect low quantities of Vismodegib in the skin after its topical application via transfersomes. Vismodegib is a novel antineoplastic drug approved for oral administration for treatment of basal cell carcinoma. Transfersomes loaded with Vismodegib were prepared by thin film resuspension and extrusion, and were characterized physicochemically. Transfersomes were applied to human and pig skin specimens using the Saarbrücken penetration model. The skin was then sectioned by tape stripping, followed by penetration assessment by UV-Vis spectroscopy and Raman spectroscopy in a confocal Raman microscope. Raman signals from Vismodegib and transfersomes were recovered from skin sections, showing a similar distribution in the <i>stratum corneum</i> obtained by the other techniques. On the other hand, pig and human skin showed differences in their penetration profiles, proving their lack of equivalence for assessing the performance of these transfersomes. Raman spectroscopy appears as a potential non-invasive, direct tool for monitoring hard-to-detect molecules in a complex environment such as the skin. |
| first_indexed | 2024-03-11T01:46:45Z |
| format | Article |
| id | doaj.art-0ebbffe9fe2640b9a40c0b23698bf2a2 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T01:46:45Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-0ebbffe9fe2640b9a40c0b23698bf2a22023-11-18T16:10:00ZengMDPI AGApplied Sciences2076-34172023-06-011313768710.3390/app13137687Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the SkinGisela Eliane Gómez0María Natalia Calienni1Silvia del Valle Alonso2Fernando Carlos Alvira3Jorge Montanari4Laboratorio de Bio-Nanotecnología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaLaboratorio de Bio-Nanotecnología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaLaboratorio de Bio-Nanotecnología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaLaboratorio de Bio-Nanotecnología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaLaboratorio de Bio-Nanotecnología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, ArgentinaRaman spectroscopy was used to detect low quantities of Vismodegib in the skin after its topical application via transfersomes. Vismodegib is a novel antineoplastic drug approved for oral administration for treatment of basal cell carcinoma. Transfersomes loaded with Vismodegib were prepared by thin film resuspension and extrusion, and were characterized physicochemically. Transfersomes were applied to human and pig skin specimens using the Saarbrücken penetration model. The skin was then sectioned by tape stripping, followed by penetration assessment by UV-Vis spectroscopy and Raman spectroscopy in a confocal Raman microscope. Raman signals from Vismodegib and transfersomes were recovered from skin sections, showing a similar distribution in the <i>stratum corneum</i> obtained by the other techniques. On the other hand, pig and human skin showed differences in their penetration profiles, proving their lack of equivalence for assessing the performance of these transfersomes. Raman spectroscopy appears as a potential non-invasive, direct tool for monitoring hard-to-detect molecules in a complex environment such as the skin.https://www.mdpi.com/2076-3417/13/13/7687Raman spectroscopyskin penetrationVismodegib |
| spellingShingle | Gisela Eliane Gómez María Natalia Calienni Silvia del Valle Alonso Fernando Carlos Alvira Jorge Montanari Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin Applied Sciences Raman spectroscopy skin penetration Vismodegib |
| title | Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin |
| title_full | Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin |
| title_fullStr | Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin |
| title_full_unstemmed | Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin |
| title_short | Raman Spectroscopy to Monitor the Delivery of a Nano-Formulation of Vismodegib in the Skin |
| title_sort | raman spectroscopy to monitor the delivery of a nano formulation of vismodegib in the skin |
| topic | Raman spectroscopy skin penetration Vismodegib |
| url | https://www.mdpi.com/2076-3417/13/13/7687 |
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