Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.)
Premise of the study: <i>Firmiana</i> consists of 12–16 species, many of which are narrow endemics. Expressed sequence tag (EST)–simple sequence repeat (SSR) markers were developed and characterized for size polymorphism in four <i>Firmiana</i> species. Methods and Results:...
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| Format: | Article |
| Language: | English |
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Wiley
2013-11-01
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| Series: | Applications in Plant Sciences |
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| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300047 |
| _version_ | 1811276678455885824 |
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| author | Qiang Fan Sufang Chen Mingwan Li Shiyang He Renchao Zhou Wenbo Liao |
| author_facet | Qiang Fan Sufang Chen Mingwan Li Shiyang He Renchao Zhou Wenbo Liao |
| author_sort | Qiang Fan |
| collection | DOAJ |
| description | Premise of the study: <i>Firmiana</i> consists of 12–16 species, many of which are narrow endemics. Expressed sequence tag (EST)–simple sequence repeat (SSR) markers were developed and characterized for size polymorphism in four <i>Firmiana</i> species.
Methods and Results: A total of 102 EST-SSR primer pairs were designed based on the transcriptome sequences of <i>F. danxiaensis</i>; these were then characterized in four <i>Firmiana</i> species—<i>F. danxiaensis</i>, <i>F. kwangsiensis</i>, <i>F. hainanensis</i>, and <i>F. simplex</i>. In these four species, 17 primer pairs were successfully amplified, and 14 were polymorphic in at least one species. The number of alleles ranged from one to 13, and the observed and expected heterozygosities ranged from 0 to 1 and 0 to 0.925, respectively. The lowest level of polymorphism was observed in <i>F. danxiaensis</i>.
Conclusions: These polymorphic EST-SSR markers are valuable for conservation genetics studies in the endangered <i>Firmiana</i> species. |
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| id | doaj.art-0ebd6999d58e4ed98cfe6c869f6848b5 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-13T00:02:05Z |
| publishDate | 2013-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-0ebd6999d58e4ed98cfe6c869f6848b52022-12-22T03:11:19ZengWileyApplications in Plant Sciences2168-04502013-11-01112130004710.3732/apps.1300047Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.)Qiang Fan0Sufang Chen1Mingwan Li2Shiyang He3Renchao Zhou4Wenbo Liao5State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaState Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaState Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaState Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaState Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaState Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of ChinaPremise of the study: <i>Firmiana</i> consists of 12–16 species, many of which are narrow endemics. Expressed sequence tag (EST)–simple sequence repeat (SSR) markers were developed and characterized for size polymorphism in four <i>Firmiana</i> species. Methods and Results: A total of 102 EST-SSR primer pairs were designed based on the transcriptome sequences of <i>F. danxiaensis</i>; these were then characterized in four <i>Firmiana</i> species—<i>F. danxiaensis</i>, <i>F. kwangsiensis</i>, <i>F. hainanensis</i>, and <i>F. simplex</i>. In these four species, 17 primer pairs were successfully amplified, and 14 were polymorphic in at least one species. The number of alleles ranged from one to 13, and the observed and expected heterozygosities ranged from 0 to 1 and 0 to 0.925, respectively. The lowest level of polymorphism was observed in <i>F. danxiaensis</i>. Conclusions: These polymorphic EST-SSR markers are valuable for conservation genetics studies in the endangered <i>Firmiana</i> species.http://www.bioone.org/doi/full/10.3732/apps.1300047Firmiana danxiaensisMalvaceaemicrosatellitestranscriptometransferability |
| spellingShingle | Qiang Fan Sufang Chen Mingwan Li Shiyang He Renchao Zhou Wenbo Liao Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) Applications in Plant Sciences Firmiana danxiaensis Malvaceae microsatellites transcriptome transferability |
| title | Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) |
| title_full | Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) |
| title_fullStr | Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) |
| title_full_unstemmed | Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) |
| title_short | Development and Characterization of Microsatellite Markers from the Transcriptome of <i>Firmiana danxiaensis</i> (Malvaceae s.l.) |
| title_sort | development and characterization of microsatellite markers from the transcriptome of i firmiana danxiaensis i malvaceae s l |
| topic | Firmiana danxiaensis Malvaceae microsatellites transcriptome transferability |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300047 |
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