Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i>
The aim of this study was to compare the reference broth microdilution (BMD) method with the Disk Diffusion (DD) test and strip test against a collection of 75 well-characterized <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>Klebsiella pneumoniae</i> (KPC-Kp)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/614 |
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| author | Federica Bovo Tiziana Lazzarotto Simone Ambretti Paolo Gaibani |
| author_facet | Federica Bovo Tiziana Lazzarotto Simone Ambretti Paolo Gaibani |
| author_sort | Federica Bovo |
| collection | DOAJ |
| description | The aim of this study was to compare the reference broth microdilution (BMD) method with the Disk Diffusion (DD) test and strip test against a collection of 75 well-characterized <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>Klebsiella pneumoniae</i> (KPC-Kp) clinical strains to assess cefiderocol (CFD) antimicrobial activity. Whole-genome sequencing was performed on KPC-Kp strains by Illumina iSeq100 platform. The Categorical Agreement (CA) between the BMD method and DD test was 92% (69/75) with a Major Error (ME) of 16.7% (6/36). Additionally, the CA between the BMD method and test strip was 90.7% (68/75) with a Very Major Error (VME) of 17.9% (7/39) and 82.7% (62/75) between the strip test and DD with a ME of 30.2%. KPC-Kp strains showing resistance to CFD were 27 out of 75 (36%) by three methods. Specifically, 51.9% (14/27) of KPC-Kp resistant to CFD harbored <i>bla</i><sub>KPC-3</sub>, while 48.1% (13/27) harbored mutated <i>bla</i><sub>KPC-3</sub>. Moreover, KPC-Kp strains carrying a mutated <i>bla</i><sub>KPC-3</sub> gene exhibited high MIC values (<i>p</i> value < 0.001) compared to wild-type <i>bla</i><sub>KPC-3</sub>. In conclusion, the DD test resulted as a valid alternative to the BMD method to determine the in vitro susceptibility to CFD, while the strip test exhibited major limitations. |
| first_indexed | 2024-03-11T07:00:10Z |
| format | Article |
| id | doaj.art-0ec2bd5ab0ec46fea380da1954cdca7d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T07:00:10Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-0ec2bd5ab0ec46fea380da1954cdca7d2023-11-17T09:15:34ZengMDPI AGAntibiotics2079-63822023-03-0112361410.3390/antibiotics12030614Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i>Federica Bovo0Tiziana Lazzarotto1Simone Ambretti2Paolo Gaibani3Microbiology Operative Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyMicrobiology Operative Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyMicrobiology Operative Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyMicrobiology Operative Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyThe aim of this study was to compare the reference broth microdilution (BMD) method with the Disk Diffusion (DD) test and strip test against a collection of 75 well-characterized <i>Klebsiella pneumoniae</i> carbapenemase (KPC)-producing <i>Klebsiella pneumoniae</i> (KPC-Kp) clinical strains to assess cefiderocol (CFD) antimicrobial activity. Whole-genome sequencing was performed on KPC-Kp strains by Illumina iSeq100 platform. The Categorical Agreement (CA) between the BMD method and DD test was 92% (69/75) with a Major Error (ME) of 16.7% (6/36). Additionally, the CA between the BMD method and test strip was 90.7% (68/75) with a Very Major Error (VME) of 17.9% (7/39) and 82.7% (62/75) between the strip test and DD with a ME of 30.2%. KPC-Kp strains showing resistance to CFD were 27 out of 75 (36%) by three methods. Specifically, 51.9% (14/27) of KPC-Kp resistant to CFD harbored <i>bla</i><sub>KPC-3</sub>, while 48.1% (13/27) harbored mutated <i>bla</i><sub>KPC-3</sub>. Moreover, KPC-Kp strains carrying a mutated <i>bla</i><sub>KPC-3</sub> gene exhibited high MIC values (<i>p</i> value < 0.001) compared to wild-type <i>bla</i><sub>KPC-3</sub>. In conclusion, the DD test resulted as a valid alternative to the BMD method to determine the in vitro susceptibility to CFD, while the strip test exhibited major limitations.https://www.mdpi.com/2079-6382/12/3/614cefiderocolantimicrobial susceptibility testing (AST)antimicrobial resistance (AMR)<i>Klebsiella pneumoniae</i> carbapenemase |
| spellingShingle | Federica Bovo Tiziana Lazzarotto Simone Ambretti Paolo Gaibani Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> Antibiotics cefiderocol antimicrobial susceptibility testing (AST) antimicrobial resistance (AMR) <i>Klebsiella pneumoniae</i> carbapenemase |
| title | Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> |
| title_full | Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> |
| title_fullStr | Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> |
| title_full_unstemmed | Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> |
| title_short | Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing <i>Klebsiella pneumoniae</i> |
| title_sort | comparison of broth microdilution disk diffusion and strip test methods for cefiderocol antimicrobial susceptibility testing on kpc producing i klebsiella pneumoniae i |
| topic | cefiderocol antimicrobial susceptibility testing (AST) antimicrobial resistance (AMR) <i>Klebsiella pneumoniae</i> carbapenemase |
| url | https://www.mdpi.com/2079-6382/12/3/614 |
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