Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site d...
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MDPI AG
2022-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/2/84 |
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| author | Hui Zhou Cong He Zhenfeng Li Jingqian Huo Yu Xue Xiaotong Xu Meng Qi Lai Chen Bruce D. Hammock Jinlin Zhang |
| author_facet | Hui Zhou Cong He Zhenfeng Li Jingqian Huo Yu Xue Xiaotong Xu Meng Qi Lai Chen Bruce D. Hammock Jinlin Zhang |
| author_sort | Hui Zhou |
| collection | DOAJ |
| description | 2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site detection method is particularly important. In order to achieve on-site rapid detection of 2,4-D, we developed a gold nanoparticle immunochromatographic strip method with the visual elimination value was 50 ng/mL, and a quantitative detection limit of 11 ng/mL based on a nanobody. By combing with the color snap, the immunochromatographic strip could quantitatively analyze the amounts of 2,4-D. Meanwhile, a colorimetric card based on the true color of the test strips was developed for the qualitative analysis of 2,4-D on-site. The samples (water, fruits and vegetables) with and without 2,4-D were detected by the immunochromatographic strips, and the results showed the accuracy and reliability. Thus, this assay is a rapid and simple on-site analytical tool to detect and quantify 2,4-D levels in environmental samples, and the analytical results can be obtained in about ten minutes. In addition, the nanobody technology used in this study provides an inexhaustible supply of a relatively stable antibodies that can be archived as a nanobody, plasmid or even its sequence. |
| first_indexed | 2024-03-09T22:28:37Z |
| format | Article |
| id | doaj.art-0ec74176b1cb41c5a189ffe02ee9ee04 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T22:28:37Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0ec74176b1cb41c5a189ffe02ee9ee042023-11-23T19:01:02ZengMDPI AGBiosensors2079-63742022-01-011228410.3390/bios12020084Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic AcidHui Zhou0Cong He1Zhenfeng Li2Jingqian Huo3Yu Xue4Xiaotong Xu5Meng Qi6Lai Chen7Bruce D. Hammock8Jinlin Zhang9College of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Entomology and Nematology, and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USACollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Entomology and Nematology, and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USACollege of Plant Protection, Hebei Agricultural University, Baoding 071001, China2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site detection method is particularly important. In order to achieve on-site rapid detection of 2,4-D, we developed a gold nanoparticle immunochromatographic strip method with the visual elimination value was 50 ng/mL, and a quantitative detection limit of 11 ng/mL based on a nanobody. By combing with the color snap, the immunochromatographic strip could quantitatively analyze the amounts of 2,4-D. Meanwhile, a colorimetric card based on the true color of the test strips was developed for the qualitative analysis of 2,4-D on-site. The samples (water, fruits and vegetables) with and without 2,4-D were detected by the immunochromatographic strips, and the results showed the accuracy and reliability. Thus, this assay is a rapid and simple on-site analytical tool to detect and quantify 2,4-D levels in environmental samples, and the analytical results can be obtained in about ten minutes. In addition, the nanobody technology used in this study provides an inexhaustible supply of a relatively stable antibodies that can be archived as a nanobody, plasmid or even its sequence.https://www.mdpi.com/2079-6374/12/2/84gold nanoparticle immunochromatographic strip2,4-Dichlorophenoxyacetic acidnanobodycolorimetric card |
| spellingShingle | Hui Zhou Cong He Zhenfeng Li Jingqian Huo Yu Xue Xiaotong Xu Meng Qi Lai Chen Bruce D. Hammock Jinlin Zhang Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid Biosensors gold nanoparticle immunochromatographic strip 2,4-Dichlorophenoxyacetic acid nanobody colorimetric card |
| title | Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid |
| title_full | Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid |
| title_fullStr | Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid |
| title_full_unstemmed | Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid |
| title_short | Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid |
| title_sort | development of a rapid gold nanoparticle immunochromatographic strip based on the nanobody for detecting 2 4 dichlorophenoxyacetic acid |
| topic | gold nanoparticle immunochromatographic strip 2,4-Dichlorophenoxyacetic acid nanobody colorimetric card |
| url | https://www.mdpi.com/2079-6374/12/2/84 |
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