Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids
Novel environmentally friendly pretreatments have been developed in recent years to improve biomass fractionation. Solid-state fermentation (SSF) and treatment with ionic liquids show low environmental impact and can be used in biorefinery of biomass. In this work, these processes were assessed with...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/22/3711 |
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| author | David Outeiriño Iván Costa-Trigo Ricardo Pinheiro de Souza Oliveira Nelson Pérez Guerra José Manuel Salgado José Manuel Domínguez |
| author_facet | David Outeiriño Iván Costa-Trigo Ricardo Pinheiro de Souza Oliveira Nelson Pérez Guerra José Manuel Salgado José Manuel Domínguez |
| author_sort | David Outeiriño |
| collection | DOAJ |
| description | Novel environmentally friendly pretreatments have been developed in recent years to improve biomass fractionation. Solid-state fermentation (SSF) and treatment with ionic liquids show low environmental impact and can be used in biorefinery of biomass. In this work, these processes were assessed with brewery spent grain (BSG). First, BSG was used as a substrate to produce cellulases and xylanases by SSF with the fungi <i>Aspergillus brasiliensis</i> CECT 2700 and <i>Trichoderma reesei</i> CECT 2414. Then, BSG was pretreated with the ionic liquid [N<sub>1112</sub>OH][Gly] and hydrolyzed with the crude enzymatic extracts. Results showed that SSF of BSG with <i>A. brasiliensis</i> achieved the highest enzyme production; meanwhile, the pretreatment with ionic liquids allowed glucan and xylan fractions to increase and reduce the lignin content. In addition, a mixture of the extracts from both fungi in a ratio of 2.5:0.5 <i>Aspergillus</i>/<i>Trichoderma</i> (<i>v</i>/<i>v</i>) efficiently hydrolyzed the BSG previously treated with the ionic liquid [N<sub>1112</sub>OH][Gly], reaching saccharification percentages of 80.68%, 54.29%, and 19.58% for glucan, xylan, and arabinan, respectively. In conclusion, the results demonstrated that the BSG biorefinery process developed in this work is an effective way to obtain fermentable sugar-containing solutions, which can be used to produce value-added products. |
| first_indexed | 2024-03-09T18:19:15Z |
| format | Article |
| id | doaj.art-0ec90020154941e18cffacc657bd37ef |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T18:19:15Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-0ec90020154941e18cffacc657bd37ef2023-11-24T08:23:04ZengMDPI AGFoods2304-81582022-11-011122371110.3390/foods11223711Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic LiquidsDavid Outeiriño0Iván Costa-Trigo1Ricardo Pinheiro de Souza Oliveira2Nelson Pérez Guerra3José Manuel Salgado4José Manuel Domínguez5Industrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, Campus Ourense, University of Vigo, 32004 Ourense, SpainIndustrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, Campus Ourense, University of Vigo, 32004 Ourense, SpainBiochemical and Pharmaceutical Technology Department, Faculty of Pharmaceutical Sciences, Sao Paulo University, Av. Prof Lineu Prestes, 580, Bl 16, Sao Paulo 05508-900, BrazilDepartment of Analytical and Food Chemistry, Faculty of Sciences, Campus Ourense, University of Vigo, As Lagoas s/n, 32004 Ourense, SpainIndustrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, Campus Ourense, University of Vigo, 32004 Ourense, SpainIndustrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, Campus Ourense, University of Vigo, 32004 Ourense, SpainNovel environmentally friendly pretreatments have been developed in recent years to improve biomass fractionation. Solid-state fermentation (SSF) and treatment with ionic liquids show low environmental impact and can be used in biorefinery of biomass. In this work, these processes were assessed with brewery spent grain (BSG). First, BSG was used as a substrate to produce cellulases and xylanases by SSF with the fungi <i>Aspergillus brasiliensis</i> CECT 2700 and <i>Trichoderma reesei</i> CECT 2414. Then, BSG was pretreated with the ionic liquid [N<sub>1112</sub>OH][Gly] and hydrolyzed with the crude enzymatic extracts. Results showed that SSF of BSG with <i>A. brasiliensis</i> achieved the highest enzyme production; meanwhile, the pretreatment with ionic liquids allowed glucan and xylan fractions to increase and reduce the lignin content. In addition, a mixture of the extracts from both fungi in a ratio of 2.5:0.5 <i>Aspergillus</i>/<i>Trichoderma</i> (<i>v</i>/<i>v</i>) efficiently hydrolyzed the BSG previously treated with the ionic liquid [N<sub>1112</sub>OH][Gly], reaching saccharification percentages of 80.68%, 54.29%, and 19.58% for glucan, xylan, and arabinan, respectively. In conclusion, the results demonstrated that the BSG biorefinery process developed in this work is an effective way to obtain fermentable sugar-containing solutions, which can be used to produce value-added products.https://www.mdpi.com/2304-8158/11/22/3711biorefinerybrewery spent grainionic liquids<i>Aspergillus brasiliensis</i><i>Trichoderma reesei</i> |
| spellingShingle | David Outeiriño Iván Costa-Trigo Ricardo Pinheiro de Souza Oliveira Nelson Pérez Guerra José Manuel Salgado José Manuel Domínguez Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids Foods biorefinery brewery spent grain ionic liquids <i>Aspergillus brasiliensis</i> <i>Trichoderma reesei</i> |
| title | Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids |
| title_full | Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids |
| title_fullStr | Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids |
| title_full_unstemmed | Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids |
| title_short | Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids |
| title_sort | biorefinery of brewery spent grain by solid state fermentation and ionic liquids |
| topic | biorefinery brewery spent grain ionic liquids <i>Aspergillus brasiliensis</i> <i>Trichoderma reesei</i> |
| url | https://www.mdpi.com/2304-8158/11/22/3711 |
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