Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)
The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several p...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2004-09-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003 |
| _version_ | 1811242471736213504 |
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| author | Leonie Asfora Sarubbo Luciana Alves de Oliveira Ana Lúcia Figueiredo Porto Galba Maria de Campos-Takaki Elias Basile Tambourgi |
| author_facet | Leonie Asfora Sarubbo Luciana Alves de Oliveira Ana Lúcia Figueiredo Porto Galba Maria de Campos-Takaki Elias Basile Tambourgi |
| author_sort | Leonie Asfora Sarubbo |
| collection | DOAJ |
| description | The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.<br>A partição de duas proteínas, albumina de soro bovino (BSA) e tripsina foi estudada no sistema bifásico aquoso Polietileno glicol(PEG) - Goma do cajueiro. O diagrama de fases foi estabelecido para a Goma do Cajueiro e para PEG de peso molecular 1500 em duas diferentes temperaturas. A influência de vários parâmetros na partição destas proteínas, incluindo concentração dos polímeros, pH, adição de sal e temperatura foi investigada. Os resultados desta pesquisa demonstraram a importância das características da proteína na partição em sistemas bifásicos aquosos. |
| first_indexed | 2024-04-12T13:51:47Z |
| format | Article |
| id | doaj.art-0eca173411ac476bb9e14e65c3645427 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-04-12T13:51:47Z |
| publishDate | 2004-09-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-0eca173411ac476bb9e14e65c36454272022-12-22T03:30:30ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242004-09-0147568569110.1590/S1516-89132004000500003Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)Leonie Asfora SarubboLuciana Alves de OliveiraAna Lúcia Figueiredo PortoGalba Maria de Campos-TakakiElias Basile TambourgiThe partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.<br>A partição de duas proteínas, albumina de soro bovino (BSA) e tripsina foi estudada no sistema bifásico aquoso Polietileno glicol(PEG) - Goma do cajueiro. O diagrama de fases foi estabelecido para a Goma do Cajueiro e para PEG de peso molecular 1500 em duas diferentes temperaturas. A influência de vários parâmetros na partição destas proteínas, incluindo concentração dos polímeros, pH, adição de sal e temperatura foi investigada. Os resultados desta pesquisa demonstraram a importância das características da proteína na partição em sistemas bifásicos aquosos.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003Aqueous two-phase systemsine serum albuminCashew-nut tree gumprotein partition |
| spellingShingle | Leonie Asfora Sarubbo Luciana Alves de Oliveira Ana Lúcia Figueiredo Porto Galba Maria de Campos-Takaki Elias Basile Tambourgi Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) Brazilian Archives of Biology and Technology Aqueous two-phase systems ine serum albumin Cashew-nut tree gum protein partition |
| title | Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) |
| title_full | Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) |
| title_fullStr | Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) |
| title_full_unstemmed | Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) |
| title_short | Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) |
| title_sort | partition of proteins in aqueous two phase systems based on cashew nut tree gum and poly ethylene glycol |
| topic | Aqueous two-phase systems ine serum albumin Cashew-nut tree gum protein partition |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003 |
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