De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea
The kiwifruit (<i>Actinidia arguta</i> var. purpurea) produces oval shaped fruits containing a slightly green or mauve outer exocarp and a purple-flesh endocarp with rows of tiny black seeds. The flesh color of the fruit results from a range of anthocyanin compounds, and is an important...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12120 |
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| author | Bei Niu Qiaohong Li Lijuan Fan Xiaodong Shi Yuan Liu Qiguo Zhuang Xiaobo Qin |
| author_facet | Bei Niu Qiaohong Li Lijuan Fan Xiaodong Shi Yuan Liu Qiguo Zhuang Xiaobo Qin |
| author_sort | Bei Niu |
| collection | DOAJ |
| description | The kiwifruit (<i>Actinidia arguta</i> var. purpurea) produces oval shaped fruits containing a slightly green or mauve outer exocarp and a purple-flesh endocarp with rows of tiny black seeds. The flesh color of the fruit results from a range of anthocyanin compounds, and is an important trait for kiwifruit consumers. To elucidate the molecular mechanisms involved in anthocyanin biosynthesis of the sarcocarp during <i>A. arguta</i> fruit development, de novo assembly and transcriptomic profile analyses were performed. Based on significant Gene Ontology (GO) biological terms, differentially expressed genes were identified in flavonoid biosynthetic and metabolic processes, pigment biosynthesis, carbohydrate metabolic processes, and amino acid metabolic processes. The genes closely related to anthocyanin biosynthesis, such as phenylalanine ammonia-lyase (<i>PAL</i>), chalcone synthase (<i>CHS</i>), and anthocyanidin synthase (<i>ANS</i>), displayed significant up-regulation during fruit development according to the transcriptomic data, which was further confirmed by qRT-PCR. Meanwhile, a series of transcription factor genes were identified among the DEGs. Through a correlation analysis. AaMYB1 was found to be significantly correlated with key genes of anthocyanin biosynthesis, especially with <i>CHS</i>. Through a transient expression assay, AaMYB1 induced anthocyanin accumulation in tobacco leaves. These data provide an important basis for exploring the related mechanisms of sarcocarp anthocyanin biosynthesis in <i>A. arguta</i>. This study will provide a strong foundation for functional studies on <i>A. arguta</i> and will facilitate improved breeding of <i>A. arguta</i> fruit. |
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| language | English |
| last_indexed | 2024-03-09T20:06:26Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0ecad888c10c4f0590aeaa4d72c56a212023-11-24T00:28:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201212010.3390/ijms232012120De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpureaBei Niu0Qiaohong Li1Lijuan Fan2Xiaodong Shi3Yuan Liu4Qiguo Zhuang5Xiaobo Qin6Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, ChinaSichuan Provincial Academy of Natural Resource Sciences, Chengdu 610015, ChinaCollege of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, ChinaSichuan Provincial Academy of Natural Resource Sciences, Chengdu 610015, ChinaSichuan Provincial Academy of Natural Resource Sciences, Chengdu 610015, ChinaKey Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, ChinaThe kiwifruit (<i>Actinidia arguta</i> var. purpurea) produces oval shaped fruits containing a slightly green or mauve outer exocarp and a purple-flesh endocarp with rows of tiny black seeds. The flesh color of the fruit results from a range of anthocyanin compounds, and is an important trait for kiwifruit consumers. To elucidate the molecular mechanisms involved in anthocyanin biosynthesis of the sarcocarp during <i>A. arguta</i> fruit development, de novo assembly and transcriptomic profile analyses were performed. Based on significant Gene Ontology (GO) biological terms, differentially expressed genes were identified in flavonoid biosynthetic and metabolic processes, pigment biosynthesis, carbohydrate metabolic processes, and amino acid metabolic processes. The genes closely related to anthocyanin biosynthesis, such as phenylalanine ammonia-lyase (<i>PAL</i>), chalcone synthase (<i>CHS</i>), and anthocyanidin synthase (<i>ANS</i>), displayed significant up-regulation during fruit development according to the transcriptomic data, which was further confirmed by qRT-PCR. Meanwhile, a series of transcription factor genes were identified among the DEGs. Through a correlation analysis. AaMYB1 was found to be significantly correlated with key genes of anthocyanin biosynthesis, especially with <i>CHS</i>. Through a transient expression assay, AaMYB1 induced anthocyanin accumulation in tobacco leaves. These data provide an important basis for exploring the related mechanisms of sarcocarp anthocyanin biosynthesis in <i>A. arguta</i>. This study will provide a strong foundation for functional studies on <i>A. arguta</i> and will facilitate improved breeding of <i>A. arguta</i> fruit.https://www.mdpi.com/1422-0067/23/20/12120transcriptomeanthocyaninregulationMYB transcription factorkiwifruit<i>Actinidia arguta</i> var. purpurea |
| spellingShingle | Bei Niu Qiaohong Li Lijuan Fan Xiaodong Shi Yuan Liu Qiguo Zhuang Xiaobo Qin De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea International Journal of Molecular Sciences transcriptome anthocyanin regulation MYB transcription factor kiwifruit <i>Actinidia arguta</i> var. purpurea |
| title | De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea |
| title_full | De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea |
| title_fullStr | De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea |
| title_full_unstemmed | De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea |
| title_short | De Novo Assembly of a Sarcocarp Transcriptome Set Identifies AaMYB1 as a Regulator of Anthocyanin Biosynthesis in <i>Actinidia arguta</i> var. purpurea |
| title_sort | de novo assembly of a sarcocarp transcriptome set identifies aamyb1 as a regulator of anthocyanin biosynthesis in i actinidia arguta i var purpurea |
| topic | transcriptome anthocyanin regulation MYB transcription factor kiwifruit <i>Actinidia arguta</i> var. purpurea |
| url | https://www.mdpi.com/1422-0067/23/20/12120 |
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