Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging
Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomogra...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Neural Circuits |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncir.2019.00074/full |
| _version_ | 1828893882858864640 |
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| author | Chihiro Inami Hiroki Tanihira Satomi Kikuta Satomi Kikuta Osamu Ogasawara Kazuya Sobue Kazuhiko Kume Makoto Osanai Makoto Osanai Makoto Osanai Masahiro Ohsawa |
| author_facet | Chihiro Inami Hiroki Tanihira Satomi Kikuta Satomi Kikuta Osamu Ogasawara Kazuya Sobue Kazuhiko Kume Makoto Osanai Makoto Osanai Makoto Osanai Masahiro Ohsawa |
| author_sort | Chihiro Inami |
| collection | DOAJ |
| description | Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomography (PET) require the use of anesthesia to suppress movement. Consequently, conclusions derived from existing imaging studies in rodents may not accurately reflect brain activity under awake conditions. In this study, we used quantitative activation-induced manganese-enhanced magnetic resonance imaging to directly capture the previous brain activity of awake mice. We also observed and quantified the brain activity of the spared nerve injury (SNI) neuropathic pain model during awake conditions. SNI-operated mice exhibited a robust decrease of mechanical nociceptive threshold 14 days after nerve injury. Imaging on SNI-operated mice revealed increased neural activity in the limbic system and secondary somatosensory, sensory-motor, piriform, and insular cortex. We present the first study demonstrating a direct measurement of awake neural activity in a neuropathic pain mouse model. |
| first_indexed | 2024-12-13T14:01:02Z |
| format | Article |
| id | doaj.art-0ecae07adb544a81a1ca31ae6f9532ea |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-13T14:01:02Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-0ecae07adb544a81a1ca31ae6f9532ea2022-12-21T23:42:43ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102019-11-011310.3389/fncir.2019.00074436254Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance ImagingChihiro Inami0Hiroki Tanihira1Satomi Kikuta2Satomi Kikuta3Osamu Ogasawara4Kazuya Sobue5Kazuhiko Kume6Makoto Osanai7Makoto Osanai8Makoto Osanai9Masahiro Ohsawa10Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, JapanGraduate School of Medicine, Tohoku University, Sendai, JapanGraduate School of Medicine, Tohoku University, Sendai, JapanSystems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, JapanDepartment of Anesthesiology, Graduate School of Medicine, Nagoya City University, Nagoya, JapanDepartment of Anesthesiology, Graduate School of Medicine, Nagoya City University, Nagoya, JapanDepartment of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, JapanGraduate School of Medicine, Tohoku University, Sendai, JapanGraduate School of Biomedical Engineering, Tohoku University, Sendai, JapanDivision of Health Sciences, Department of Medical Physics and Engineering, Graduate School of Medicine, Osaka University, Suita, JapanDepartment of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, JapanHuman brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomography (PET) require the use of anesthesia to suppress movement. Consequently, conclusions derived from existing imaging studies in rodents may not accurately reflect brain activity under awake conditions. In this study, we used quantitative activation-induced manganese-enhanced magnetic resonance imaging to directly capture the previous brain activity of awake mice. We also observed and quantified the brain activity of the spared nerve injury (SNI) neuropathic pain model during awake conditions. SNI-operated mice exhibited a robust decrease of mechanical nociceptive threshold 14 days after nerve injury. Imaging on SNI-operated mice revealed increased neural activity in the limbic system and secondary somatosensory, sensory-motor, piriform, and insular cortex. We present the first study demonstrating a direct measurement of awake neural activity in a neuropathic pain mouse model.https://www.frontiersin.org/article/10.3389/fncir.2019.00074/fullneuropathic painMRImanganesesomatosensory abnormalityemotion |
| spellingShingle | Chihiro Inami Hiroki Tanihira Satomi Kikuta Satomi Kikuta Osamu Ogasawara Kazuya Sobue Kazuhiko Kume Makoto Osanai Makoto Osanai Makoto Osanai Masahiro Ohsawa Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging Frontiers in Neural Circuits neuropathic pain MRI manganese somatosensory abnormality emotion |
| title | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
| title_full | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
| title_fullStr | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
| title_full_unstemmed | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
| title_short | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
| title_sort | visualization of brain activity in a neuropathic pain model using quantitative activity dependent manganese magnetic resonance imaging |
| topic | neuropathic pain MRI manganese somatosensory abnormality emotion |
| url | https://www.frontiersin.org/article/10.3389/fncir.2019.00074/full |
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