Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil
Leishmaniasis is an important metazoonosis caused by protozoa of the genus Leishmania and has a heteroxenic life cycle involving invertebrate and vertebrate hosts. Transmission occurs during the blood meal of infected phlebotomine sand flies in wild species, domestic animals, and humans. The dog is...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2014-12-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/17009 |
| _version_ | 1818394769098801152 |
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| author | Caroline Constantino Aline Kuhn Sbruzzi Pasquali Eloiza Teles Caldart Fernanda Pinto Ferreira Elizabete Regina Marangoni Marana Roberta Lemos Freire Regina Mitsuka-Breganó Carmen Lúcia Scortecci Hilst Odilon Vidotto Italmar Teodorico Navarro |
| author_facet | Caroline Constantino Aline Kuhn Sbruzzi Pasquali Eloiza Teles Caldart Fernanda Pinto Ferreira Elizabete Regina Marangoni Marana Roberta Lemos Freire Regina Mitsuka-Breganó Carmen Lúcia Scortecci Hilst Odilon Vidotto Italmar Teodorico Navarro |
| author_sort | Caroline Constantino |
| collection | DOAJ |
| description | Leishmaniasis is an important metazoonosis caused by protozoa of the genus Leishmania and has a heteroxenic life cycle involving invertebrate and vertebrate hosts. Transmission occurs during the blood meal of infected phlebotomine sand flies in wild species, domestic animals, and humans. The dog is a reservoir for the parasite causing visceral leishmaniasis (VL), whereas in American tegumentary leishmaniasis (ATL), dogs are erratic hosts that are accidentally infected, as in humans. Dogs are considered an important indicator of the parasite and its vectors in the environment, thus highlighting the importance of diagnosis in these animals. This study aimed to assess the seroepidemiology of Leishmania spp. in dogs in the municipality of Telêmaco Borba that were part of a castration campaign. Blood samples from 191 dogs were collected, and their owners were surveyed on various epidemiological variables. Serological analysis was performed using indirect immunofluorescence (IIF) and rapid immunochromatography (DPP®). Screening by IIF identified 13 (6.81%) positive animals, none of which were positive for the DPP® test, which is specific for VL. Statistical analysis of the questionnaire responses indicated a significant association between seropositivity and the presence of stacked or composting leaves in the backyard (p = 0.0498), forest areas (squares, woods, parks) near the residence (p = 0.0015), and poorly healing ulcerated or nodular epidermal lesions in the dog (p = 0.0138). This study revealed the presence of anti-Leishmania spp. IgG antibodies in dogs residing in Telêmaco Borba, suggesting the presence of the parasite and vector in the environment. In addition, the existence of stacked or composting leaves in the backyard, forest areas near the residence, and epidermal lesions in dogs are factors associated with Leishmania spp. infection in pet dogs. |
| first_indexed | 2024-12-14T06:06:28Z |
| format | Article |
| id | doaj.art-0ecb54b9140d410bb2a2fa960889e2f1 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-14T06:06:28Z |
| publishDate | 2014-12-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-0ecb54b9140d410bb2a2fa960889e2f12022-12-21T23:14:16ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592014-12-013563181319010.5433/1679-0359.2014v35n6p3181Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, BrazilCaroline Constantino 0Aline Kuhn Sbruzzi Pasquali 1Eloiza Teles Caldart 2Fernanda Pinto Ferreira 3Elizabete Regina Marangoni Marana 4Roberta Lemos Freire 5 Regina Mitsuka-Breganó 6 Carmen Lúcia Scortecci Hilst 7Odilon Vidotto8Italmar Teodorico Navarro 9Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaLeishmaniasis is an important metazoonosis caused by protozoa of the genus Leishmania and has a heteroxenic life cycle involving invertebrate and vertebrate hosts. Transmission occurs during the blood meal of infected phlebotomine sand flies in wild species, domestic animals, and humans. The dog is a reservoir for the parasite causing visceral leishmaniasis (VL), whereas in American tegumentary leishmaniasis (ATL), dogs are erratic hosts that are accidentally infected, as in humans. Dogs are considered an important indicator of the parasite and its vectors in the environment, thus highlighting the importance of diagnosis in these animals. This study aimed to assess the seroepidemiology of Leishmania spp. in dogs in the municipality of Telêmaco Borba that were part of a castration campaign. Blood samples from 191 dogs were collected, and their owners were surveyed on various epidemiological variables. Serological analysis was performed using indirect immunofluorescence (IIF) and rapid immunochromatography (DPP®). Screening by IIF identified 13 (6.81%) positive animals, none of which were positive for the DPP® test, which is specific for VL. Statistical analysis of the questionnaire responses indicated a significant association between seropositivity and the presence of stacked or composting leaves in the backyard (p = 0.0498), forest areas (squares, woods, parks) near the residence (p = 0.0015), and poorly healing ulcerated or nodular epidermal lesions in the dog (p = 0.0138). This study revealed the presence of anti-Leishmania spp. IgG antibodies in dogs residing in Telêmaco Borba, suggesting the presence of the parasite and vector in the environment. In addition, the existence of stacked or composting leaves in the backyard, forest areas near the residence, and epidermal lesions in dogs are factors associated with Leishmania spp. infection in pet dogs.http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/17009Leishmaniasis; Pet dogs; Epidemiology; Indirect immunofluorescence; Rapid immunochromatography test; Diagnosis |
| spellingShingle | Caroline Constantino Aline Kuhn Sbruzzi Pasquali Eloiza Teles Caldart Fernanda Pinto Ferreira Elizabete Regina Marangoni Marana Roberta Lemos Freire Regina Mitsuka-Breganó Carmen Lúcia Scortecci Hilst Odilon Vidotto Italmar Teodorico Navarro Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil Semina: Ciências Agrárias Leishmaniasis; Pet dogs; Epidemiology; Indirect immunofluorescence; Rapid immunochromatography test; Diagnosis |
| title | Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil |
| title_full | Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil |
| title_fullStr | Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil |
| title_full_unstemmed | Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil |
| title_short | Seroepidemiology of Leishmania spp. in dogs residing in Telêmaco Borba, Paraná, Brazil |
| title_sort | seroepidemiology of leishmania spp in dogs residing in telemaco borba parana brazil |
| topic | Leishmaniasis; Pet dogs; Epidemiology; Indirect immunofluorescence; Rapid immunochromatography test; Diagnosis |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/17009 |
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