Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples

CFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y. pestis antigen detection in field material from the territory of the Altai mountain natural plague focus...

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Main Authors: O. A. Noskova, T. Yu. Zagoskina, E. N. Subycheva, E. Yu. Markov, Yu. O. Popova, L. G. Gridneva, E. P. Mikhailov
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2014-09-01
Series:Проблемы особо опасных инфекций
Subjects:
Online Access:https://journal.microbe.ru/jour/article/view/167
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author O. A. Noskova
T. Yu. Zagoskina
E. N. Subycheva
E. Yu. Markov
Yu. O. Popova
L. G. Gridneva
E. P. Mikhailov
author_facet O. A. Noskova
T. Yu. Zagoskina
E. N. Subycheva
E. Yu. Markov
Yu. O. Popova
L. G. Gridneva
E. P. Mikhailov
author_sort O. A. Noskova
collection DOAJ
description CFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y. pestis antigen detection in field material from the territory of the Altai mountain natural plague focus by dot-immunoassay with comparison of the received results in passive hemagglutination reaction. Test-systems possessed a number of advantages as compared to routine serological reactions and could be applied with success by practical public health services both in stationary and field conditions.
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series Проблемы особо опасных инфекций
spelling doaj.art-0ed1427fb8bf44649be43f31963dd66a2024-12-24T12:09:51ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-09-0103697110.21055/0370-1069-2014-3-69-71167Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field SamplesO. A. Noskova0T. Yu. Zagoskina1E. N. Subycheva2E. Yu. Markov3Yu. O. Popova4L. G. Gridneva5E. P. Mikhailov6Irkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastAltai Plague Control StationCFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y. pestis antigen detection in field material from the territory of the Altai mountain natural plague focus by dot-immunoassay with comparison of the received results in passive hemagglutination reaction. Test-systems possessed a number of advantages as compared to routine serological reactions and could be applied with success by practical public health services both in stationary and field conditions.https://journal.microbe.ru/jour/article/view/167plague agentspecific antibodiescolloid silverdot-immunoassay
spellingShingle O. A. Noskova
T. Yu. Zagoskina
E. N. Subycheva
E. Yu. Markov
Yu. O. Popova
L. G. Gridneva
E. P. Mikhailov
Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
Проблемы особо опасных инфекций
plague agent
specific antibodies
colloid silver
dot-immunoassay
title Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
title_full Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
title_fullStr Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
title_full_unstemmed Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
title_short Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples
title_sort application of dot immunoassay for detection of plague agent antigens in the field samples
topic plague agent
specific antibodies
colloid silver
dot-immunoassay
url https://journal.microbe.ru/jour/article/view/167
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