Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation
Carolina laurel cherry (Prunus caroliniana) is native to the U.S. southeastern coastal plain from North Carolina westward to eastern Texas. The species has been planted extensively in the southeast as an ornamental tree or hedge. Unfortunately, carolina laurel cherry naturalizes readily and is now f...
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| Format: | Article |
| Language: | English |
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American Society for Horticultural Science (ASHS)
2019-08-01
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| Series: | HortTechnology |
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| Online Access: | https://journals.ashs.org/horttech/view/journals/horttech/29/5/article-p535.xml |
| _version_ | 1818316456120549376 |
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| author | John M. Ruter |
| author_facet | John M. Ruter |
| author_sort | John M. Ruter |
| collection | DOAJ |
| description | Carolina laurel cherry (Prunus caroliniana) is native to the U.S. southeastern coastal plain from North Carolina westward to eastern Texas. The species has been planted extensively in the southeast as an ornamental tree or hedge. Unfortunately, carolina laurel cherry naturalizes readily and is now found in a variety of habitats, both natural and disturbed. Flowering occurs in the late winter/early spring before new leaves emerge and fruit ripens in the fall/winter. Fruit is eaten by migratory birds and seed is dispersed. Seedlings readily germinate in the understory of forests and landscapes in the spring. As there are a limited number of cultivars available, selections with improved form and sterility are needed for the landscape trade. In 2008, seed was collected and treated with Cobalt-60 gamma irradiation at rates ranging from 0 to 150 Gy. The lethal dose killing 50% of the seedlings (LD50) was between 50 and 100 Gy. Three sterile plants were selected in 2012 from the M1 (first generation of mutagen-treated seedlings) population totaling 62 seedlings. M2 (second-generation seedlings from M1 parents) seed was collected Fall 2012, and 1509 seedlings were grown to flowering size in containers. In 2014–15, 120 seedlings that showed no fruit production were planted in the field in Watkinsville, GA, for further evaluation. Ratings on field-grown plants in Dec. 2017 and 2018 showed that 73% and 78% of the plants, respectively, produced no fruit, whereas the remaining plants had minimal to heavy fruit set. Because carolina laurel cherry is andromonoecious, production of male and bisexual flowers was evaluated on 17 selections in 2018. Of 500 flowers evaluated per selection, the number of male flowers per plant ranged from 22 to 415 (4.4% to 83%). The number of racemes with all-male flowers on each selection ranged from 1 to 32. There were no significant correlations between the number of male flowers or number of all-male flowered racemes per plant and production of fruit. Approximately 5% of M2 seedlings remain seedless after 6 years of growth. |
| first_indexed | 2024-12-13T09:21:43Z |
| format | Article |
| id | doaj.art-0ed42c400f204a38a1fc8e1129ce6354 |
| institution | Directory Open Access Journal |
| issn | 1943-7714 |
| language | English |
| last_indexed | 2024-12-13T09:21:43Z |
| publishDate | 2019-08-01 |
| publisher | American Society for Horticultural Science (ASHS) |
| record_format | Article |
| series | HortTechnology |
| spelling | doaj.art-0ed42c400f204a38a1fc8e1129ce63542022-12-21T23:52:43ZengAmerican Society for Horticultural Science (ASHS)HortTechnology1943-77142019-08-01295535538https://doi.org/10.21273/HORTTECH04289-19Inducing Sterility in Carolina Laurel Cherry Using Gamma IrradiationJohn M. Ruter Carolina laurel cherry (Prunus caroliniana) is native to the U.S. southeastern coastal plain from North Carolina westward to eastern Texas. The species has been planted extensively in the southeast as an ornamental tree or hedge. Unfortunately, carolina laurel cherry naturalizes readily and is now found in a variety of habitats, both natural and disturbed. Flowering occurs in the late winter/early spring before new leaves emerge and fruit ripens in the fall/winter. Fruit is eaten by migratory birds and seed is dispersed. Seedlings readily germinate in the understory of forests and landscapes in the spring. As there are a limited number of cultivars available, selections with improved form and sterility are needed for the landscape trade. In 2008, seed was collected and treated with Cobalt-60 gamma irradiation at rates ranging from 0 to 150 Gy. The lethal dose killing 50% of the seedlings (LD50) was between 50 and 100 Gy. Three sterile plants were selected in 2012 from the M1 (first generation of mutagen-treated seedlings) population totaling 62 seedlings. M2 (second-generation seedlings from M1 parents) seed was collected Fall 2012, and 1509 seedlings were grown to flowering size in containers. In 2014–15, 120 seedlings that showed no fruit production were planted in the field in Watkinsville, GA, for further evaluation. Ratings on field-grown plants in Dec. 2017 and 2018 showed that 73% and 78% of the plants, respectively, produced no fruit, whereas the remaining plants had minimal to heavy fruit set. Because carolina laurel cherry is andromonoecious, production of male and bisexual flowers was evaluated on 17 selections in 2018. Of 500 flowers evaluated per selection, the number of male flowers per plant ranged from 22 to 415 (4.4% to 83%). The number of racemes with all-male flowers on each selection ranged from 1 to 32. There were no significant correlations between the number of male flowers or number of all-male flowered racemes per plant and production of fruit. Approximately 5% of M2 seedlings remain seedless after 6 years of growth.https://journals.ashs.org/horttech/view/journals/horttech/29/5/article-p535.xmlandromonoecycobalt-60floweringgerminationprunus carolinianaseed |
| spellingShingle | John M. Ruter Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation HortTechnology andromonoecy cobalt-60 flowering germination prunus caroliniana seed |
| title | Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation |
| title_full | Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation |
| title_fullStr | Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation |
| title_full_unstemmed | Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation |
| title_short | Inducing Sterility in Carolina Laurel Cherry Using Gamma Irradiation |
| title_sort | inducing sterility in carolina laurel cherry using gamma irradiation |
| topic | andromonoecy cobalt-60 flowering germination prunus caroliniana seed |
| url | https://journals.ashs.org/horttech/view/journals/horttech/29/5/article-p535.xml |
| work_keys_str_mv | AT johnmruter inducingsterilityincarolinalaurelcherryusinggammairradiation |