Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes
The effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken IGFBP2 coding region was synthesized by overlap extension PCR and c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2019-07-01
|
| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/56/3/56_0180114/_html/-char/en |
| _version_ | 1797816986485391360 |
|---|---|
| author | Yanshuang Mu Yuhang Sun Li Leng Zhiping Cao Yumao Li Hui Li Yuxiang Wang |
| author_facet | Yanshuang Mu Yuhang Sun Li Leng Zhiping Cao Yumao Li Hui Li Yuxiang Wang |
| author_sort | Yanshuang Mu |
| collection | DOAJ |
| description | The effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken IGFBP2 coding region was synthesized by overlap extension PCR and cloned into the pcDNA3.1 vector. An in situ digestion method was used to prepare the chicken hepatocytes. Primary chicken hepatocytes were maintained in monolayer culture. Real-time PCR was used to detect changes in the expression of IGFBP2, PPARG, IGF1, IGF1R, APOAI, and LFABP, after the overexpression of IGFBP2 in chicken hepatocytes. Triglyceride production and glucose content were also evaluated using triglyceride and glucose analysis methods. The expression level of IGFBP2 increased after transfection of the IGFBP2-containing vector. The expression levels of PPARG, IGF1, and IGF1R also increased in cultured chicken hepatocytes after the overexpression of IGFBP2, whereas the expression of LFABP and APOAI decreased. Triglyceride production in primary cultured chicken hepatocytes increased after the overexpression of IGFBP2. These results suggest that IGFBP2 is involved in lipogenesis, increasing both the expression of fatty acid synthesis regulators, and triglyceride production in primary cultured chicken hepatocytes. |
| first_indexed | 2024-03-13T08:46:42Z |
| format | Article |
| id | doaj.art-0ed7075b33cb42f488c182046e34a2eb |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:42Z |
| publishDate | 2019-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-0ed7075b33cb42f488c182046e34a2eb2023-05-30T01:29:31ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862019-07-0156317718510.2141/jpsa.0180114jpsaEffect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken HepatocytesYanshuang Mu0Yuhang Sun1Li Leng2Zhiping Cao3Yumao Li4Hui Li5Yuxiang Wang6Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural UniversityThe effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken IGFBP2 coding region was synthesized by overlap extension PCR and cloned into the pcDNA3.1 vector. An in situ digestion method was used to prepare the chicken hepatocytes. Primary chicken hepatocytes were maintained in monolayer culture. Real-time PCR was used to detect changes in the expression of IGFBP2, PPARG, IGF1, IGF1R, APOAI, and LFABP, after the overexpression of IGFBP2 in chicken hepatocytes. Triglyceride production and glucose content were also evaluated using triglyceride and glucose analysis methods. The expression level of IGFBP2 increased after transfection of the IGFBP2-containing vector. The expression levels of PPARG, IGF1, and IGF1R also increased in cultured chicken hepatocytes after the overexpression of IGFBP2, whereas the expression of LFABP and APOAI decreased. Triglyceride production in primary cultured chicken hepatocytes increased after the overexpression of IGFBP2. These results suggest that IGFBP2 is involved in lipogenesis, increasing both the expression of fatty acid synthesis regulators, and triglyceride production in primary cultured chicken hepatocytes.https://www.jstage.jst.go.jp/article/jpsa/56/3/56_0180114/_html/-char/enchickenhepatocyteinsulin-like growth factor binding protein 2triglyceride |
| spellingShingle | Yanshuang Mu Yuhang Sun Li Leng Zhiping Cao Yumao Li Hui Li Yuxiang Wang Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes The Journal of Poultry Science chicken hepatocyte insulin-like growth factor binding protein 2 triglyceride |
| title | Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes |
| title_full | Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes |
| title_fullStr | Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes |
| title_full_unstemmed | Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes |
| title_short | Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes |
| title_sort | effect of igfbp2 overexpression on the expression of fatty acid synthesis genes in primary cultured chicken hepatocytes |
| topic | chicken hepatocyte insulin-like growth factor binding protein 2 triglyceride |
| url | https://www.jstage.jst.go.jp/article/jpsa/56/3/56_0180114/_html/-char/en |
| work_keys_str_mv | AT yanshuangmu effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT yuhangsun effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT lileng effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT zhipingcao effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT yumaoli effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT huili effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes AT yuxiangwang effectofigfbp2overexpressionontheexpressionoffattyacidsynthesisgenesinprimaryculturedchickenhepatocytes |