Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival
<p>Abstract</p> <p>Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza (HPAI) H5N1 (local strain) virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 10<sup...
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| Format: | Article |
| Language: | English |
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BMC
2009-03-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/38 |
| _version_ | 1818562754540208128 |
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| author | Hameed Sajid Abubakar Muhammad Shahid Muhammad Hassan Shamsul |
| author_facet | Hameed Sajid Abubakar Muhammad Shahid Muhammad Hassan Shamsul |
| author_sort | Hameed Sajid |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza (HPAI) H5N1 (local strain) virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 10<sup>8.3 </sup>ELD<sub>50</sub>/ml was mixed with sterilized peptone water to get final dilution of 4HA units and then exposed to physical (temperature, pH and ultraviolet light) and chemical (formalin, phenol crystals, iodine crystals, CID 20, virkon<sup>®</sup>-S, zeptin 10%, KEPCIDE 300, KEPCIDE 400, lifebuoy, surf excel and caustic soda) agents. Harvested amnio-allantoic fluid (AAF) from embryonated chicken eggs inoculated with H5N1 treated virus (0.2 ml/egg) was subjected to haemagglutination (HA) and haemagglutination inhibition (HI) tests. H5N1 virus lost infectivity after 30 min at 56°C, after 1 day at 28°C but remained viable for more than 100 days at 4°C. Acidic pH (1, 3) and basic pH (11, 13) were virucidal after 6 h contact time; however virus retained infectivity at pH 5 (18 h), 7 and 9 (more than 24 h). UV light was proved ineffectual in inactivating virus completely even after 60 min. Soap (lifebuoy<sup>®</sup>), detergent (surf excel<sup>®</sup>) and alkali (caustic soda) destroyed infectivity after 5 min at 0.1, 0.2 and 0.3% dilution. All commercially available disinfectants inactivated virus at recommended concentrations. Results of present study would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake of avian influenza virus (AIV) outbreak.</p> |
| first_indexed | 2024-12-14T01:07:54Z |
| format | Article |
| id | doaj.art-0ed7e8d5a78249978c8f078a4d7e539a |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-14T01:07:54Z |
| publishDate | 2009-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-0ed7e8d5a78249978c8f078a4d7e539a2022-12-21T23:22:54ZengBMCVirology Journal1743-422X2009-03-01613810.1186/1743-422X-6-38Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survivalHameed SajidAbubakar MuhammadShahid MuhammadHassan Shamsul<p>Abstract</p> <p>Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza (HPAI) H5N1 (local strain) virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 10<sup>8.3 </sup>ELD<sub>50</sub>/ml was mixed with sterilized peptone water to get final dilution of 4HA units and then exposed to physical (temperature, pH and ultraviolet light) and chemical (formalin, phenol crystals, iodine crystals, CID 20, virkon<sup>®</sup>-S, zeptin 10%, KEPCIDE 300, KEPCIDE 400, lifebuoy, surf excel and caustic soda) agents. Harvested amnio-allantoic fluid (AAF) from embryonated chicken eggs inoculated with H5N1 treated virus (0.2 ml/egg) was subjected to haemagglutination (HA) and haemagglutination inhibition (HI) tests. H5N1 virus lost infectivity after 30 min at 56°C, after 1 day at 28°C but remained viable for more than 100 days at 4°C. Acidic pH (1, 3) and basic pH (11, 13) were virucidal after 6 h contact time; however virus retained infectivity at pH 5 (18 h), 7 and 9 (more than 24 h). UV light was proved ineffectual in inactivating virus completely even after 60 min. Soap (lifebuoy<sup>®</sup>), detergent (surf excel<sup>®</sup>) and alkali (caustic soda) destroyed infectivity after 5 min at 0.1, 0.2 and 0.3% dilution. All commercially available disinfectants inactivated virus at recommended concentrations. Results of present study would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake of avian influenza virus (AIV) outbreak.</p>http://www.virologyj.com/content/6/1/38 |
| spellingShingle | Hameed Sajid Abubakar Muhammad Shahid Muhammad Hassan Shamsul Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival Virology Journal |
| title | Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival |
| title_full | Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival |
| title_fullStr | Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival |
| title_full_unstemmed | Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival |
| title_short | Avian influenza virus (H<sub>5</sub>N<sub>1</sub>); effects of physico-chemical factors on its survival |
| title_sort | avian influenza virus h sub 5 sub n sub 1 sub effects of physico chemical factors on its survival |
| url | http://www.virologyj.com/content/6/1/38 |
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