Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases
Hybrid carriers with the mineral CaCO<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> core and the protein–tannin shell are attractive for drug delivery applications due to reliable coupling of anticancer drugs with protein–tannin complex and the possibility of remote con...
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MDPI AG
2022-05-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/7/2/61 |
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| author | Polina A. Demina Mariia S. Saveleva Roman A. Anisimov Ekaterina S. Prikhozhdenko Denis V. Voronin Anatolii A. Abalymov Kirill A. Cherednichenko Olesya I. Timaeva Maria V. Lomova |
| author_facet | Polina A. Demina Mariia S. Saveleva Roman A. Anisimov Ekaterina S. Prikhozhdenko Denis V. Voronin Anatolii A. Abalymov Kirill A. Cherednichenko Olesya I. Timaeva Maria V. Lomova |
| author_sort | Polina A. Demina |
| collection | DOAJ |
| description | Hybrid carriers with the mineral CaCO<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> core and the protein–tannin shell are attractive for drug delivery applications due to reliable coupling of anticancer drugs with protein–tannin complex and the possibility of remote control over drug localization and delivery by the external magnetic field. This study aims to elucidate the mechanisms of drug release via enzymatic degradation of a protein–tannin carrier shell triggered by proteolytic hydrolases trypsin and pepsin under physiological conditions. To do this, the carriers were incubated with the enzyme solutions in special buffers to maintain the enzyme activity. The time-lapse spectrophotometric and electron microscopy measurements were carried out to evaluate the degradation of the carriers. It was established that the protein–tannin complex demonstrates the different degradation behavior depending on the enzyme type and buffer medium. The incubation in trypsin solution mostly resulted in the protein shell degradation. The incubation in pepsin solution did not affect the protein component; however, the citric buffer stimulates the degradation of the mineral core. The presented results allow for predicting the degradation pathways of the carriers including the release profile of the loaded cargo under physiological conditions. The viability of 4T1 breast cancer cells with mineral magnetic carriers with protein–tannin shells was investigated, and their movement in the fields of action of the permanent magnet was shown. |
| first_indexed | 2024-03-10T00:18:35Z |
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| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-10T00:18:35Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-0ed8b2abd5024db5a9665314e99e32cd2023-11-23T15:45:41ZengMDPI AGBiomimetics2313-76732022-05-01726110.3390/biomimetics7020061Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic HydrolasesPolina A. Demina0Mariia S. Saveleva1Roman A. Anisimov2Ekaterina S. Prikhozhdenko3Denis V. Voronin4Anatolii A. Abalymov5Kirill A. Cherednichenko6Olesya I. Timaeva7Maria V. Lomova8Science Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaDepartment of Physical and Colloid Chemistry, National University of Oil and Gas «Gubkin University», Leninsky Prospekt 65, 119991 Moscow, RussiaNational Research Center “Kurchatov Institute”, 123182 Moscow, RussiaScience Medical Centre, Saratov State University, Astrakhanskaya St. 83, 410012 Saratov, RussiaHybrid carriers with the mineral CaCO<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> core and the protein–tannin shell are attractive for drug delivery applications due to reliable coupling of anticancer drugs with protein–tannin complex and the possibility of remote control over drug localization and delivery by the external magnetic field. This study aims to elucidate the mechanisms of drug release via enzymatic degradation of a protein–tannin carrier shell triggered by proteolytic hydrolases trypsin and pepsin under physiological conditions. To do this, the carriers were incubated with the enzyme solutions in special buffers to maintain the enzyme activity. The time-lapse spectrophotometric and electron microscopy measurements were carried out to evaluate the degradation of the carriers. It was established that the protein–tannin complex demonstrates the different degradation behavior depending on the enzyme type and buffer medium. The incubation in trypsin solution mostly resulted in the protein shell degradation. The incubation in pepsin solution did not affect the protein component; however, the citric buffer stimulates the degradation of the mineral core. The presented results allow for predicting the degradation pathways of the carriers including the release profile of the loaded cargo under physiological conditions. The viability of 4T1 breast cancer cells with mineral magnetic carriers with protein–tannin shells was investigated, and their movement in the fields of action of the permanent magnet was shown.https://www.mdpi.com/2313-7673/7/2/61drug delivery systemsproteinpolyphenoltannic acidproteolytic enzymecalcium carbonate |
| spellingShingle | Polina A. Demina Mariia S. Saveleva Roman A. Anisimov Ekaterina S. Prikhozhdenko Denis V. Voronin Anatolii A. Abalymov Kirill A. Cherednichenko Olesya I. Timaeva Maria V. Lomova Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases Biomimetics drug delivery systems protein polyphenol tannic acid proteolytic enzyme calcium carbonate |
| title | Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases |
| title_full | Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases |
| title_fullStr | Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases |
| title_full_unstemmed | Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases |
| title_short | Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases |
| title_sort | degradation of hybrid drug delivery carriers with a mineral core and a protein tannin shell under proteolytic hydrolases |
| topic | drug delivery systems protein polyphenol tannic acid proteolytic enzyme calcium carbonate |
| url | https://www.mdpi.com/2313-7673/7/2/61 |
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