Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
The use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was st...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.760476/full |
| _version_ | 1818722207117869056 |
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| author | Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han |
| author_facet | Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han |
| author_sort | Hee Seo |
| collection | DOAJ |
| description | The use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was stable under human gastrointestinal conditions. In in vitro experiments, EFEL6901 cells adhered well to colonic epithelial cells and decreased nitric oxide production in lipopolysaccharide-induced macrophages. In in vivo experiments, oral administration of EFEL6901 to DSS-induced colitis mice models significantly alleviated the observed colitis symptoms, prevented body weight loss, lowered the disease activity index score, and prevented colon length shortening. Analysis of these results indicated that EFEL6901 played a probiotic role by preventing the overproduction of pro-inflammatory cytokines, improving gut barrier function, and up-regulating the concentrations of short-chain fatty acids. In addition, EFEL6901 made a fast growth in a simulated kimchi juice and it synthesized similar amounts of metabolites in nabak-kimchi comparable to a commercial kimchi. This study demonstrates that EFEL6901 can be used as a suitable kimchi starter to promote gut health and product quality. |
| first_indexed | 2024-12-17T20:50:57Z |
| format | Article |
| id | doaj.art-0edac61f07804a9490acf528d7ff52cf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T20:50:57Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0edac61f07804a9490acf528d7ff52cf2022-12-21T21:33:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.760476760476Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo EvidenceHee Seo0Hyunbin Seong1Ga Yun Kim2Yu Mi Jo3Seong Won Cheon4Youngju Song5Byung Hee Ryu6Hee Kang7Nam Soo Han8Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaBrain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaBrain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaBrain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaBrain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaDepartment of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, South KoreaFresh Food Research Division, Food BU, Daesang Corporation Research Institute, Icheon, South KoreaHumanitas College, Kyung Hee University, Yongin, South KoreaBrain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, South KoreaThe use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was stable under human gastrointestinal conditions. In in vitro experiments, EFEL6901 cells adhered well to colonic epithelial cells and decreased nitric oxide production in lipopolysaccharide-induced macrophages. In in vivo experiments, oral administration of EFEL6901 to DSS-induced colitis mice models significantly alleviated the observed colitis symptoms, prevented body weight loss, lowered the disease activity index score, and prevented colon length shortening. Analysis of these results indicated that EFEL6901 played a probiotic role by preventing the overproduction of pro-inflammatory cytokines, improving gut barrier function, and up-regulating the concentrations of short-chain fatty acids. In addition, EFEL6901 made a fast growth in a simulated kimchi juice and it synthesized similar amounts of metabolites in nabak-kimchi comparable to a commercial kimchi. This study demonstrates that EFEL6901 can be used as a suitable kimchi starter to promote gut health and product quality.https://www.frontiersin.org/articles/10.3389/fmicb.2021.760476/fullLimosilactobacillus reuteriprobiotickimchi starterinflammatory bowel diseaseDSS-induced colitisanti-inflammation |
| spellingShingle | Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence Frontiers in Microbiology Limosilactobacillus reuteri probiotic kimchi starter inflammatory bowel disease DSS-induced colitis anti-inflammation |
| title | Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence |
| title_full | Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence |
| title_fullStr | Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence |
| title_full_unstemmed | Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence |
| title_short | Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence |
| title_sort | development of anti inflammatory probiotic limosilactobacillus reuteri efel6901 as kimchi starter in vitro and in vivo evidence |
| topic | Limosilactobacillus reuteri probiotic kimchi starter inflammatory bowel disease DSS-induced colitis anti-inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.760476/full |
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