MicroRNAs dynamically remodel gastrointestinal smooth muscle cells.
Smooth muscle cells (SMCs) express a unique set of microRNAs (miRNAs) which regulate and maintain the differentiation state of SMCs. The goal of this study was to investigate the role of miRNAs during the development of gastrointestinal (GI) SMCs in a transgenic animal model. We generated SMC-specif...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-04-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3077387?pdf=render |
| _version_ | 1818271480872435712 |
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| author | Chanjae Park Wei Yan Sean M Ward Sung Jin Hwang Qiuxia Wu William J Hatton Jong Kun Park Kenton M Sanders Seungil Ro |
| author_facet | Chanjae Park Wei Yan Sean M Ward Sung Jin Hwang Qiuxia Wu William J Hatton Jong Kun Park Kenton M Sanders Seungil Ro |
| author_sort | Chanjae Park |
| collection | DOAJ |
| description | Smooth muscle cells (SMCs) express a unique set of microRNAs (miRNAs) which regulate and maintain the differentiation state of SMCs. The goal of this study was to investigate the role of miRNAs during the development of gastrointestinal (GI) SMCs in a transgenic animal model. We generated SMC-specific Dicer null animals that express the reporter, green fluorescence protein, in a SMC-specific manner. SMC-specific knockout of Dicer prevented SMC miRNA biogenesis, causing dramatic changes in phenotype, function, and global gene expression in SMCs: the mutant mice developed severe dilation of the intestinal tract associated with the thinning and destruction of the smooth muscle (SM) layers; contractile motility in the mutant intestine was dramatically decreased; and SM contractile genes and transcriptional regulators were extensively down-regulated in the mutant SMCs. Profiling and bioinformatic analyses showed that SMC phenotype is regulated by a complex network of positive and negative feedback by SMC miRNAs, serum response factor (SRF), and other transcriptional factors. Taken together, our data suggest that SMC miRNAs are required for the development and survival of SMCs in the GI tract. |
| first_indexed | 2024-12-12T21:26:51Z |
| format | Article |
| id | doaj.art-0edfdd3b553544cca185fd0d78dfb690 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T21:26:51Z |
| publishDate | 2011-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0edfdd3b553544cca185fd0d78dfb6902022-12-22T00:11:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1862810.1371/journal.pone.0018628MicroRNAs dynamically remodel gastrointestinal smooth muscle cells.Chanjae ParkWei YanSean M WardSung Jin HwangQiuxia WuWilliam J HattonJong Kun ParkKenton M SandersSeungil RoSmooth muscle cells (SMCs) express a unique set of microRNAs (miRNAs) which regulate and maintain the differentiation state of SMCs. The goal of this study was to investigate the role of miRNAs during the development of gastrointestinal (GI) SMCs in a transgenic animal model. We generated SMC-specific Dicer null animals that express the reporter, green fluorescence protein, in a SMC-specific manner. SMC-specific knockout of Dicer prevented SMC miRNA biogenesis, causing dramatic changes in phenotype, function, and global gene expression in SMCs: the mutant mice developed severe dilation of the intestinal tract associated with the thinning and destruction of the smooth muscle (SM) layers; contractile motility in the mutant intestine was dramatically decreased; and SM contractile genes and transcriptional regulators were extensively down-regulated in the mutant SMCs. Profiling and bioinformatic analyses showed that SMC phenotype is regulated by a complex network of positive and negative feedback by SMC miRNAs, serum response factor (SRF), and other transcriptional factors. Taken together, our data suggest that SMC miRNAs are required for the development and survival of SMCs in the GI tract.http://europepmc.org/articles/PMC3077387?pdf=render |
| spellingShingle | Chanjae Park Wei Yan Sean M Ward Sung Jin Hwang Qiuxia Wu William J Hatton Jong Kun Park Kenton M Sanders Seungil Ro MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. PLoS ONE |
| title | MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. |
| title_full | MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. |
| title_fullStr | MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. |
| title_full_unstemmed | MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. |
| title_short | MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. |
| title_sort | micrornas dynamically remodel gastrointestinal smooth muscle cells |
| url | http://europepmc.org/articles/PMC3077387?pdf=render |
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