Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics
<i>Listeria monocytogenes</i> is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of <i>L. monocytogenes</i> subtypes and strains. Comparison of the genome sequences among <i>...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-08-01
|
Series: | Toxins |
Subjects: | |
Online Access: | https://www.mdpi.com/2072-6651/11/9/508 |
_version_ | 1798042035198885888 |
---|---|
author | Hossam Abdelhamed Mark L. Lawrence Reshma Ramachandran Attila Karsi |
author_facet | Hossam Abdelhamed Mark L. Lawrence Reshma Ramachandran Attila Karsi |
author_sort | Hossam Abdelhamed |
collection | DOAJ |
description | <i>Listeria monocytogenes</i> is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of <i>L. monocytogenes</i> subtypes and strains. Comparison of the genome sequences among <i>L. monocytogenes</i> pathogenic strains EGD-e and F2365 with nonpathogenic <i>L. innocua</i> CLIP1182 and <i>L. monocytogenes</i> strain HCC23 revealed a set of proteins that were present in pathogenic strains and had no orthologs among the nonpathogenic strains. Among the candidate virulence factors are five proteins: putrescine carbamoyltransferase; InlH/InlC2 family class 1 internalin; phosphotransferase system (PTS) fructose transporter subunit EIIC; putative transketolase; and transcription antiterminator BglG family. To determine if these proteins have a role in adherence and invasion of intestinal epithelial Caco-2 cells and/or contribute to virulence, five mutant strains were constructed. F2365Δ<i>inlC2</i>, F2365Δ<i>eiic</i>, and F2365Δ<i>tkt</i> exhibited a significant (<i>p</i> < 0.05) reduction in adhesion to Caco-2 cells compared to parent F2365 strain. The invasion of F2365Δ<i>aguB</i>, F2365Δ<i>inlC2</i>, and F2365Δ<i>bglG</i> decreased significantly (<i>p</i> < 0.05) compared with the parent strain. Bacterial loads in mouse liver and spleen infected by F2365 was significantly (<i>p</i> < 0.05) higher than it was for F2365Δ<i>aguB</i>, F2365Δ<i>inlC2</i>, F2365Δ<i>eiic</i>, F2365Δ<i>tkt</i>, and F2365Δ<i>bglG</i> strains. This study demonstrates that <i>aguB</i>, <i>inlC2</i>, <i>eiic</i>, <i>tkt</i>, and <i>bglG</i> play a role in <i>L. monocytogenes</i> pathogenicity. |
first_indexed | 2024-04-11T22:30:49Z |
format | Article |
id | doaj.art-0ee04c2318d043a280d2b8e437ac2ba1 |
institution | Directory Open Access Journal |
issn | 2072-6651 |
language | English |
last_indexed | 2024-04-11T22:30:49Z |
publishDate | 2019-08-01 |
publisher | MDPI AG |
record_format | Article |
series | Toxins |
spelling | doaj.art-0ee04c2318d043a280d2b8e437ac2ba12022-12-22T03:59:26ZengMDPI AGToxins2072-66512019-08-0111950810.3390/toxins11090508toxins11090508Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative GenomicsHossam Abdelhamed0Mark L. Lawrence1Reshma Ramachandran2Attila Karsi3Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USADepartment of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USADepartment of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USADepartment of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA<i>Listeria monocytogenes</i> is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of <i>L. monocytogenes</i> subtypes and strains. Comparison of the genome sequences among <i>L. monocytogenes</i> pathogenic strains EGD-e and F2365 with nonpathogenic <i>L. innocua</i> CLIP1182 and <i>L. monocytogenes</i> strain HCC23 revealed a set of proteins that were present in pathogenic strains and had no orthologs among the nonpathogenic strains. Among the candidate virulence factors are five proteins: putrescine carbamoyltransferase; InlH/InlC2 family class 1 internalin; phosphotransferase system (PTS) fructose transporter subunit EIIC; putative transketolase; and transcription antiterminator BglG family. To determine if these proteins have a role in adherence and invasion of intestinal epithelial Caco-2 cells and/or contribute to virulence, five mutant strains were constructed. F2365Δ<i>inlC2</i>, F2365Δ<i>eiic</i>, and F2365Δ<i>tkt</i> exhibited a significant (<i>p</i> < 0.05) reduction in adhesion to Caco-2 cells compared to parent F2365 strain. The invasion of F2365Δ<i>aguB</i>, F2365Δ<i>inlC2</i>, and F2365Δ<i>bglG</i> decreased significantly (<i>p</i> < 0.05) compared with the parent strain. Bacterial loads in mouse liver and spleen infected by F2365 was significantly (<i>p</i> < 0.05) higher than it was for F2365Δ<i>aguB</i>, F2365Δ<i>inlC2</i>, F2365Δ<i>eiic</i>, F2365Δ<i>tkt</i>, and F2365Δ<i>bglG</i> strains. This study demonstrates that <i>aguB</i>, <i>inlC2</i>, <i>eiic</i>, <i>tkt</i>, and <i>bglG</i> play a role in <i>L. monocytogenes</i> pathogenicity.https://www.mdpi.com/2072-6651/11/9/508<i>Listeria monocytogenes</i>adhesioninvasionmice infection |
spellingShingle | Hossam Abdelhamed Mark L. Lawrence Reshma Ramachandran Attila Karsi Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics Toxins <i>Listeria monocytogenes</i> adhesion invasion mice infection |
title | Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics |
title_full | Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics |
title_fullStr | Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics |
title_full_unstemmed | Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics |
title_short | Validation of Predicted Virulence Factors in <em>Listeria monocytogenes</em> Identified Using Comparative Genomics |
title_sort | validation of predicted virulence factors in em listeria monocytogenes em identified using comparative genomics |
topic | <i>Listeria monocytogenes</i> adhesion invasion mice infection |
url | https://www.mdpi.com/2072-6651/11/9/508 |
work_keys_str_mv | AT hossamabdelhamed validationofpredictedvirulencefactorsinemlisteriamonocytogenesemidentifiedusingcomparativegenomics AT markllawrence validationofpredictedvirulencefactorsinemlisteriamonocytogenesemidentifiedusingcomparativegenomics AT reshmaramachandran validationofpredictedvirulencefactorsinemlisteriamonocytogenesemidentifiedusingcomparativegenomics AT attilakarsi validationofpredictedvirulencefactorsinemlisteriamonocytogenesemidentifiedusingcomparativegenomics |