Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia
Abstract Background Bluetongue (BT), a vector‐borne disease of wild and domestic ruminants, is responsible for severe economic losses in flocks. To reduce this impact, a surveillance and control plan was implemented in Tunisia. However, the epidemiological situation of BT remains incompletely unders...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.818 |
| _version_ | 1817970571904811008 |
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| author | Kalthoum Sana Sghaier Soufien Ben Hassine Thameur Teodori Liana Spedicato Massimo Guesmi Kaouther Gharbi Raja Hajlaoui Haikel Bel Haj Mohamed Bassem Khalfaoui Wiem Lachtar Monia Ben Salem Ameni Fatnassi Naouel Dhaouadi Anissa Ben Ali Mehdi Thabet Sarah Seghaier Chedia Savini Giovanni Hammami Salah |
| author_facet | Kalthoum Sana Sghaier Soufien Ben Hassine Thameur Teodori Liana Spedicato Massimo Guesmi Kaouther Gharbi Raja Hajlaoui Haikel Bel Haj Mohamed Bassem Khalfaoui Wiem Lachtar Monia Ben Salem Ameni Fatnassi Naouel Dhaouadi Anissa Ben Ali Mehdi Thabet Sarah Seghaier Chedia Savini Giovanni Hammami Salah |
| author_sort | Kalthoum Sana |
| collection | DOAJ |
| description | Abstract Background Bluetongue (BT), a vector‐borne disease of wild and domestic ruminants, is responsible for severe economic losses in flocks. To reduce this impact, a surveillance and control plan was implemented in Tunisia. However, the epidemiological situation of BT remains incompletely understood, especially for the circulating serotypes. Objective The aim of this survey was to determine the seroprevalence, to identify the circulating serotypes and to identify the associated risk factors for bluetongue virus (BTV) circulation in Tunisia using risk‐based sampling (RBS). Methods A total of 3314 blood samples were randomly collected from 67 sectors using risk‐based sampling and screened by competitive enzyme‐linked immunosorbent assays (c‐ELISAs). Out of the 1330 positive samples, 200 samples were analysed by serum neutralization test (SNT) to identify circulating BTV serotypes. Results Of 3314 sera, 1330 were c‐ELISA‐positive (40.1%) for antibodies against the BTV structural protein VP7. The result of SNT showed the presence of BTV‐1, BTV‐2, BTV‐3, BTV‐4 and, for the first time in Tunisia, BTV‐26. The logistic regression model revealed that older animals had nearly two times the odds of being infected with BTV compared to younger animals. Flocks with a history of BT were almost 1.5 times more likely to be at risk for contracting BTV infection. The flock size, housing indoors and intensive production system were significant protective factors. Conclusions High seroprevalence of BTV among sheep was highlighted in Tunisia. The neutralization test showed the presence of the following BTV serotypes: BTV‐1, BTV‐2, BTV‐3, BTV‐4 and, for the first time in Tunisia, BTV‐26. Age, production system and flock size were important variables associated with BTV infection in sheep. This finding is crucial, as it will allow the adjustment of the BT control programme in Tunisia. |
| first_indexed | 2024-04-13T20:35:52Z |
| format | Article |
| id | doaj.art-0ee2c4ed61b14b3b953bf903f83aa209 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-13T20:35:52Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-0ee2c4ed61b14b3b953bf903f83aa2092022-12-22T02:31:02ZengWileyVeterinary Medicine and Science2053-10952022-07-01841671168210.1002/vms3.818Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in TunisiaKalthoum Sana0Sghaier Soufien1Ben Hassine Thameur2Teodori Liana3Spedicato Massimo4Guesmi Kaouther5Gharbi Raja6Hajlaoui Haikel7Bel Haj Mohamed Bassem8Khalfaoui Wiem9Lachtar Monia10Ben Salem Ameni11Fatnassi Naouel12Dhaouadi Anissa13Ben Ali Mehdi14Thabet Sarah15Seghaier Chedia16Savini Giovanni17Hammami Salah18National Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaInstitut de la Recherche Vétérinaire de Tunisie Tunis TunisiaDirection Générale des Services Vétérinaires Commissariat Régional au Développement Agricole de Nabeul Nabeul TunisiaIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale Teramo ItalyIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale Teramo ItalyNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaInstitut de la Recherche Vétérinaire de Tunisie Tunis TunisiaNational Centre of Zoosanitary Vigilance Ministère de L'agriculture et des Ressources Hydrauliques Tunis TunisiaIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale Teramo ItalyService de Microbiologie, Immunologie et Pathologie Générale, École Nationale de Médecine Vétérinaire de Sidi Thabet Tunisia Universitè de la Manouba Manouba TunisiaAbstract Background Bluetongue (BT), a vector‐borne disease of wild and domestic ruminants, is responsible for severe economic losses in flocks. To reduce this impact, a surveillance and control plan was implemented in Tunisia. However, the epidemiological situation of BT remains incompletely understood, especially for the circulating serotypes. Objective The aim of this survey was to determine the seroprevalence, to identify the circulating serotypes and to identify the associated risk factors for bluetongue virus (BTV) circulation in Tunisia using risk‐based sampling (RBS). Methods A total of 3314 blood samples were randomly collected from 67 sectors using risk‐based sampling and screened by competitive enzyme‐linked immunosorbent assays (c‐ELISAs). Out of the 1330 positive samples, 200 samples were analysed by serum neutralization test (SNT) to identify circulating BTV serotypes. Results Of 3314 sera, 1330 were c‐ELISA‐positive (40.1%) for antibodies against the BTV structural protein VP7. The result of SNT showed the presence of BTV‐1, BTV‐2, BTV‐3, BTV‐4 and, for the first time in Tunisia, BTV‐26. The logistic regression model revealed that older animals had nearly two times the odds of being infected with BTV compared to younger animals. Flocks with a history of BT were almost 1.5 times more likely to be at risk for contracting BTV infection. The flock size, housing indoors and intensive production system were significant protective factors. Conclusions High seroprevalence of BTV among sheep was highlighted in Tunisia. The neutralization test showed the presence of the following BTV serotypes: BTV‐1, BTV‐2, BTV‐3, BTV‐4 and, for the first time in Tunisia, BTV‐26. Age, production system and flock size were important variables associated with BTV infection in sheep. This finding is crucial, as it will allow the adjustment of the BT control programme in Tunisia.https://doi.org/10.1002/vms3.818bluetonguerisk factorsrisk‐based samplingserotypingsheepTunisia |
| spellingShingle | Kalthoum Sana Sghaier Soufien Ben Hassine Thameur Teodori Liana Spedicato Massimo Guesmi Kaouther Gharbi Raja Hajlaoui Haikel Bel Haj Mohamed Bassem Khalfaoui Wiem Lachtar Monia Ben Salem Ameni Fatnassi Naouel Dhaouadi Anissa Ben Ali Mehdi Thabet Sarah Seghaier Chedia Savini Giovanni Hammami Salah Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia Veterinary Medicine and Science bluetongue risk factors risk‐based sampling serotyping sheep Tunisia |
| title | Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia |
| title_full | Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia |
| title_fullStr | Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia |
| title_full_unstemmed | Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia |
| title_short | Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia |
| title_sort | risk based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in tunisia |
| topic | bluetongue risk factors risk‐based sampling serotyping sheep Tunisia |
| url | https://doi.org/10.1002/vms3.818 |
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