The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111.
Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3502180?pdf=render |
| _version_ | 1817998574311440384 |
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| author | Nadine Schmid Gabriella Pessi Yinyue Deng Claudio Aguilar Aurelien L Carlier Alexander Grunau Ulrich Omasits Lian-Hui Zhang Christian H Ahrens Leo Eberl |
| author_facet | Nadine Schmid Gabriella Pessi Yinyue Deng Claudio Aguilar Aurelien L Carlier Alexander Grunau Ulrich Omasits Lian-Hui Zhang Christian H Ahrens Leo Eberl |
| author_sort | Nadine Schmid |
| collection | DOAJ |
| description | Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production. |
| first_indexed | 2024-04-14T02:55:21Z |
| format | Article |
| id | doaj.art-0ee69b76c29647f2a5156f95bace7d40 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T02:55:21Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0ee69b76c29647f2a5156f95bace7d402022-12-22T02:16:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4996610.1371/journal.pone.0049966The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111.Nadine SchmidGabriella PessiYinyue DengClaudio AguilarAurelien L CarlierAlexander GrunauUlrich OmasitsLian-Hui ZhangChristian H AhrensLeo EberlQuorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production.http://europepmc.org/articles/PMC3502180?pdf=render |
| spellingShingle | Nadine Schmid Gabriella Pessi Yinyue Deng Claudio Aguilar Aurelien L Carlier Alexander Grunau Ulrich Omasits Lian-Hui Zhang Christian H Ahrens Leo Eberl The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. PLoS ONE |
| title | The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. |
| title_full | The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. |
| title_fullStr | The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. |
| title_full_unstemmed | The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. |
| title_short | The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. |
| title_sort | ahl and bdsf dependent quorum sensing systems control specific and overlapping sets of genes in burkholderia cenocepacia h111 |
| url | http://europepmc.org/articles/PMC3502180?pdf=render |
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