Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Abstract Background Mammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex proteins, produce them at high titers, and confer post-translational modifications (PTMs) critical to proper function. Increasing demand for protei...

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Bibliographic Details
Main Authors: Erica A. Green, Nathaniel K. Hamaker, Kelvin H. Lee
Format: Article
Language:English
Published: BMC 2023-03-01
Series:BMC Biotechnology
Subjects:
HEK293
SARS-CoV-2 receptor binding domain (RBD)
EBNA1
Recombinant protein production
Process development
Viral vector production host
Online Access:https://doi.org/10.1186/s12896-023-00777-7

Internet

https://doi.org/10.1186/s12896-023-00777-7

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