Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein
Abstract Background Mammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex proteins, produce them at high titers, and confer post-translational modifications (PTMs) critical to proper function. Increasing demand for protei...
Main Authors: | Erica A. Green, Nathaniel K. Hamaker, Kelvin H. Lee |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-03-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12896-023-00777-7 |
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