Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia
Abstract Background Metabolomics is a tool that has been used for the diagnosis and prognosis of specific diseases. The purpose of this study was to examine if metabolomics could be used as a potential diagnostic and prognostic tool for H1N1 pneumonia. Our hypothesis was that metabolomics can potent...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Critical Care |
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| Online Access: | http://link.springer.com/article/10.1186/s13054-017-1672-7 |
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| author | Mohammad M. Banoei Hans J. Vogel Aalim M. Weljie Anand Kumar Sachin Yende Derek C. Angus Brent W. Winston the Canadian Critical Care Translational Biology Group (CCCTBG) |
| author_facet | Mohammad M. Banoei Hans J. Vogel Aalim M. Weljie Anand Kumar Sachin Yende Derek C. Angus Brent W. Winston the Canadian Critical Care Translational Biology Group (CCCTBG) |
| author_sort | Mohammad M. Banoei |
| collection | DOAJ |
| description | Abstract Background Metabolomics is a tool that has been used for the diagnosis and prognosis of specific diseases. The purpose of this study was to examine if metabolomics could be used as a potential diagnostic and prognostic tool for H1N1 pneumonia. Our hypothesis was that metabolomics can potentially be used early for the diagnosis and prognosis of H1N1 influenza pneumonia. Methods 1H nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry were used to profile the metabolome in 42 patients with H1N1 pneumonia, 31 ventilated control subjects in the intensive care unit (ICU), and 30 culture-positive plasma samples from patients with bacterial community-acquired pneumonia drawn within the first 24 h of hospital admission for diagnosis and prognosis of disease. Results We found that plasma-based metabolomics from samples taken within 24 h of hospital admission can be used to discriminate H1N1 pneumonia from bacterial pneumonia and nonsurvivors from survivors of H1N1 pneumonia. Moreover, metabolomics is a highly sensitive and specific tool for the 90-day prognosis of mortality in H1N1 pneumonia. Conclusions This study demonstrates that H1N1 pneumonia can create a quite different plasma metabolic profile from bacterial culture-positive pneumonia and ventilated control subjects in the ICU on the basis of plasma samples taken within 24 h of hospital/ICU admission, early in the course of disease. |
| first_indexed | 2024-12-12T21:19:03Z |
| format | Article |
| id | doaj.art-0ef37f8fb07a4709bc3fb7054bb108db |
| institution | Directory Open Access Journal |
| issn | 1364-8535 |
| language | English |
| last_indexed | 2024-12-12T21:19:03Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Critical Care |
| spelling | doaj.art-0ef37f8fb07a4709bc3fb7054bb108db2022-12-22T00:11:39ZengBMCCritical Care1364-85352017-04-0121111510.1186/s13054-017-1672-7Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumoniaMohammad M. Banoei0Hans J. Vogel1Aalim M. Weljie2Anand Kumar3Sachin Yende4Derek C. Angus5Brent W. Winston6the Canadian Critical Care Translational Biology Group (CCCTBG)Department of Critical Care Medicine, University of CalgaryBio-NMR Centre, Centre for Advanced Technologies, Department of Biochemistry and Molecular Biology, University of CalgaryBio-NMR Centre, Centre for Advanced Technologies, Department of Biochemistry and Molecular Biology, University of CalgarySection of Critical Care Medicine, Winnipeg Health Sciences Centre, St. Boniface Hospital, University of ManitobaClinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Center, Department of Critical Care Medicine, University of PittsburghClinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Center, Department of Critical Care Medicine, University of PittsburghDepartment of Critical Care Medicine, University of CalgaryAbstract Background Metabolomics is a tool that has been used for the diagnosis and prognosis of specific diseases. The purpose of this study was to examine if metabolomics could be used as a potential diagnostic and prognostic tool for H1N1 pneumonia. Our hypothesis was that metabolomics can potentially be used early for the diagnosis and prognosis of H1N1 influenza pneumonia. Methods 1H nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry were used to profile the metabolome in 42 patients with H1N1 pneumonia, 31 ventilated control subjects in the intensive care unit (ICU), and 30 culture-positive plasma samples from patients with bacterial community-acquired pneumonia drawn within the first 24 h of hospital admission for diagnosis and prognosis of disease. Results We found that plasma-based metabolomics from samples taken within 24 h of hospital admission can be used to discriminate H1N1 pneumonia from bacterial pneumonia and nonsurvivors from survivors of H1N1 pneumonia. Moreover, metabolomics is a highly sensitive and specific tool for the 90-day prognosis of mortality in H1N1 pneumonia. Conclusions This study demonstrates that H1N1 pneumonia can create a quite different plasma metabolic profile from bacterial culture-positive pneumonia and ventilated control subjects in the ICU on the basis of plasma samples taken within 24 h of hospital/ICU admission, early in the course of disease.http://link.springer.com/article/10.1186/s13054-017-1672-7H1N1 pneumoniaMetabolomicsNMRGC-MSBiomarkers |
| spellingShingle | Mohammad M. Banoei Hans J. Vogel Aalim M. Weljie Anand Kumar Sachin Yende Derek C. Angus Brent W. Winston the Canadian Critical Care Translational Biology Group (CCCTBG) Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia Critical Care H1N1 pneumonia Metabolomics NMR GC-MS Biomarkers |
| title | Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia |
| title_full | Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia |
| title_fullStr | Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia |
| title_full_unstemmed | Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia |
| title_short | Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia |
| title_sort | plasma metabolomics for the diagnosis and prognosis of h1n1 influenza pneumonia |
| topic | H1N1 pneumonia Metabolomics NMR GC-MS Biomarkers |
| url | http://link.springer.com/article/10.1186/s13054-017-1672-7 |
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