Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.)
The <i>FAR1-RELATED SEQUENCE1</i> (<i>FAR1</i>) and <i>FAR-RED ELONGATED HYPOCOTYL3</i> (<i>FHY3</i>) gene family plays a crucial role in various physiological and developmental processes, including seed germination, photomorphogenesis, flowering and s...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/1/50 |
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| author | Xuelian Li Yihua Li Yali Qiao Siting Lu Kangding Yao Chunlei Wang Weibiao Liao |
| author_facet | Xuelian Li Yihua Li Yali Qiao Siting Lu Kangding Yao Chunlei Wang Weibiao Liao |
| author_sort | Xuelian Li |
| collection | DOAJ |
| description | The <i>FAR1-RELATED SEQUENCE1</i> (<i>FAR1</i>) and <i>FAR-RED ELONGATED HYPOCOTYL3</i> (<i>FHY3</i>) gene family plays a crucial role in various physiological and developmental processes, including seed germination, photomorphogenesis, flowering and stress responses. However, genome analysis of <i>FAR1/FHY3</i> in cucumber (<i>Cucumis sativus</i> L.) has not been systemically investigated. In this study, 20 <i>FAR1/FHY3</i> genes in cucumber were identified. The 20 <i>FAR1/FHY3</i> members are randomly distributed on six chromosomes. The examination of subcellular localization indicated that the nucleus is the primary site where the 20 <i>FAR1/FHY3</i> members are predominantly found. The analysis of the phylogenetic tree further revealed that the <i>FAR1/FHY3</i> genes in cucumber are grouped into three distinct categories, exhibiting remarkable resemblance to the corresponding genes in other plant species. The analysis of cis-acting elements showed that most <i>FAR1/FHY3</i> genes contain a variety of hormones as well as stress-related and light response elements. Through scrutinizing the expression patterns in various tissues, it was discerned that these genes are prominently expressed in roots, stems and leaves, with roots exhibiting the highest level of expression. Additionally, the 20 cucumber <i>FAR1/FHY3</i> genes are all responsive to jasmonic acid methyl ester (Me-JA) and abscisic acid (ABA). <i>CsFAR6</i> and <i>CsFAR12</i> are significantly induced by Me-JA and ABA, respectively. <i>CsFAR13</i> positively responds to NaCl and PEG6000 stresses. <i>CsFAR11</i>, <i>CsFAR15</i> and <i>CsFAR13</i> are significantly induced by the dark. The findings presented in this study establish compelling support for the potential involvement of <i>FAR1/FHY3</i> genes in the growth, development and stress response of cucumbers. Moreover, these results serve as a solid basis for future investigations into the functional analysis of <i>FAR1/FHY3</i>. |
| first_indexed | 2024-03-08T11:08:06Z |
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| id | doaj.art-0ef386ce95464cae8625118b765ea326 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-08T11:08:06Z |
| publishDate | 2023-12-01 |
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| series | Agronomy |
| spelling | doaj.art-0ef386ce95464cae8625118b765ea3262024-01-26T14:21:59ZengMDPI AGAgronomy2073-43952023-12-011415010.3390/agronomy14010050Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.)Xuelian Li0Yihua Li1Yali Qiao2Siting Lu3Kangding Yao4Chunlei Wang5Weibiao Liao6College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaThe <i>FAR1-RELATED SEQUENCE1</i> (<i>FAR1</i>) and <i>FAR-RED ELONGATED HYPOCOTYL3</i> (<i>FHY3</i>) gene family plays a crucial role in various physiological and developmental processes, including seed germination, photomorphogenesis, flowering and stress responses. However, genome analysis of <i>FAR1/FHY3</i> in cucumber (<i>Cucumis sativus</i> L.) has not been systemically investigated. In this study, 20 <i>FAR1/FHY3</i> genes in cucumber were identified. The 20 <i>FAR1/FHY3</i> members are randomly distributed on six chromosomes. The examination of subcellular localization indicated that the nucleus is the primary site where the 20 <i>FAR1/FHY3</i> members are predominantly found. The analysis of the phylogenetic tree further revealed that the <i>FAR1/FHY3</i> genes in cucumber are grouped into three distinct categories, exhibiting remarkable resemblance to the corresponding genes in other plant species. The analysis of cis-acting elements showed that most <i>FAR1/FHY3</i> genes contain a variety of hormones as well as stress-related and light response elements. Through scrutinizing the expression patterns in various tissues, it was discerned that these genes are prominently expressed in roots, stems and leaves, with roots exhibiting the highest level of expression. Additionally, the 20 cucumber <i>FAR1/FHY3</i> genes are all responsive to jasmonic acid methyl ester (Me-JA) and abscisic acid (ABA). <i>CsFAR6</i> and <i>CsFAR12</i> are significantly induced by Me-JA and ABA, respectively. <i>CsFAR13</i> positively responds to NaCl and PEG6000 stresses. <i>CsFAR11</i>, <i>CsFAR15</i> and <i>CsFAR13</i> are significantly induced by the dark. The findings presented in this study establish compelling support for the potential involvement of <i>FAR1/FHY3</i> genes in the growth, development and stress response of cucumbers. Moreover, these results serve as a solid basis for future investigations into the functional analysis of <i>FAR1/FHY3</i>.https://www.mdpi.com/2073-4395/14/1/50cucumberphotorespirationfunction analysisjasmonic acid methyl esterabscisic acidabiotic stress |
| spellingShingle | Xuelian Li Yihua Li Yali Qiao Siting Lu Kangding Yao Chunlei Wang Weibiao Liao Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) Agronomy cucumber photorespiration function analysis jasmonic acid methyl ester abscisic acid abiotic stress |
| title | Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) |
| title_full | Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) |
| title_fullStr | Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) |
| title_short | Genome-Wide Identification and Expression Analysis of <i>FAR1/FHY3</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.) |
| title_sort | genome wide identification and expression analysis of i far1 fhy3 i gene family in cucumber i cucumis sativus i l |
| topic | cucumber photorespiration function analysis jasmonic acid methyl ester abscisic acid abiotic stress |
| url | https://www.mdpi.com/2073-4395/14/1/50 |
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