Extracellular matrix supports healing of transected rabbit Achilles tendon
Extracellular matrix (ECM) has been intensively used in cardio surgery. The main goal of this research was to determine if Achilles tendon healing could be promoted by applying extracellular matrix scaffold (CorMatrix®, USA). Sixteen (n = 16) New Zealand white mature rabbits (Oryctolagus cuniculus)...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018324939 |
| _version_ | 1818849052495708160 |
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| author | Marija Lipar Boris Zdilar Mario Kreszinger Marijana Ćorić Berislav Radišić Marko Samardžija Rado Žic Marko Pećin |
| author_facet | Marija Lipar Boris Zdilar Mario Kreszinger Marijana Ćorić Berislav Radišić Marko Samardžija Rado Žic Marko Pećin |
| author_sort | Marija Lipar |
| collection | DOAJ |
| description | Extracellular matrix (ECM) has been intensively used in cardio surgery. The main goal of this research was to determine if Achilles tendon healing could be promoted by applying extracellular matrix scaffold (CorMatrix®, USA). Sixteen (n = 16) New Zealand white mature rabbits (Oryctolagus cuniculus) were randomly allocated into two groups. Following complete surgical transection, rabbits in group A (ECM applied) (n = 8) had their Achilles tendons reconstructed using both, nylon suture and extracellular matrix scaffold, whereas in group B (without ECM) the tendons were reconstructed using nylon suture only. After four weeks, the rabbits were euthanized and tendon samples harvested and stained with hematoxylin eosin, Mallory, and Gomory and subsequently histologically analyzed according to modified Bonnar scale. Group B had significantly stronger inflammatory response, including abundant cell infiltration and neovascularization. In group A collagen fibers were predominantly found, whereas in group B reticular fibers were more abundant. Extracellular matrix scaffold has been found to have the real potential for promoting tendon healing through accelerating collagen formation, which is crucial for restoring biomechanical properties of a tendon, decreasing peritendineous adhesion formation, and reducing inflammatory edema and subsequently pain. |
| first_indexed | 2024-12-19T06:27:06Z |
| format | Article |
| id | doaj.art-0ef4e5e494b74743a31ef839dd226186 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T06:27:06Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0ef4e5e494b74743a31ef839dd2261862022-12-21T20:32:30ZengElsevierHeliyon2405-84402018-09-0149e00781Extracellular matrix supports healing of transected rabbit Achilles tendonMarija Lipar0Boris Zdilar1Mario Kreszinger2Marijana Ćorić3Berislav Radišić4Marko Samardžija5Rado Žic6Marko Pećin7Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaHospital Sveti Duh, Ulica Sveti Duh 1, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaSchool of Medicine, University of Zagreb, Ulica Šalata 2, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia; Corresponding author.School of Medicine, University of Zagreb, Ulica Šalata 2, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaExtracellular matrix (ECM) has been intensively used in cardio surgery. The main goal of this research was to determine if Achilles tendon healing could be promoted by applying extracellular matrix scaffold (CorMatrix®, USA). Sixteen (n = 16) New Zealand white mature rabbits (Oryctolagus cuniculus) were randomly allocated into two groups. Following complete surgical transection, rabbits in group A (ECM applied) (n = 8) had their Achilles tendons reconstructed using both, nylon suture and extracellular matrix scaffold, whereas in group B (without ECM) the tendons were reconstructed using nylon suture only. After four weeks, the rabbits were euthanized and tendon samples harvested and stained with hematoxylin eosin, Mallory, and Gomory and subsequently histologically analyzed according to modified Bonnar scale. Group B had significantly stronger inflammatory response, including abundant cell infiltration and neovascularization. In group A collagen fibers were predominantly found, whereas in group B reticular fibers were more abundant. Extracellular matrix scaffold has been found to have the real potential for promoting tendon healing through accelerating collagen formation, which is crucial for restoring biomechanical properties of a tendon, decreasing peritendineous adhesion formation, and reducing inflammatory edema and subsequently pain.http://www.sciencedirect.com/science/article/pii/S2405844018324939Surgery |
| spellingShingle | Marija Lipar Boris Zdilar Mario Kreszinger Marijana Ćorić Berislav Radišić Marko Samardžija Rado Žic Marko Pećin Extracellular matrix supports healing of transected rabbit Achilles tendon Heliyon Surgery |
| title | Extracellular matrix supports healing of transected rabbit Achilles tendon |
| title_full | Extracellular matrix supports healing of transected rabbit Achilles tendon |
| title_fullStr | Extracellular matrix supports healing of transected rabbit Achilles tendon |
| title_full_unstemmed | Extracellular matrix supports healing of transected rabbit Achilles tendon |
| title_short | Extracellular matrix supports healing of transected rabbit Achilles tendon |
| title_sort | extracellular matrix supports healing of transected rabbit achilles tendon |
| topic | Surgery |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018324939 |
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